Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine
Introduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broadspectrum cephalosporin, monobactam, and penicillin. This study investigated ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the Gaza strip and explored their susceptibility to various antimicrobials to provi...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2021-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-362-en.html |
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| Summary: | Introduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broadspectrum
cephalosporin, monobactam, and penicillin. This study investigated
ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the
Gaza strip and explored their susceptibility to various antimicrobials to provide a
reference for physicians in managing the hospital infection. Methods: Ninetysix
isolates, comprising 69 E. coli and 27 K. pneumoniae were obtained from
urine, wound, blood, and ear discharge samples from April-June 2013 in Gaza
hospitals. The ESBL-producing isolates were screened using the double-disc
diffusion test. Antibiotics susceptibility test was determined by the disc diffusion
method on Mueller-Hinton agar, and PCR identified β-lactamases genes.
Results: Our results revealed high rates of ESBL-producing K. pneumoniae
(59.3%) and E. coli (39.1%) among isolates. About 65.1% of ESBL-producing
isolates were susceptible to imipenem while exhibited 100% resistance to
cefotaxime and ampicillin and 74.4% to sulfamethoxazole/trimethoprim. Except
for imipenem, higher antibiotic resistance rates were observed among ESBL
producers than non-ESBL producers. This study showed that the antimicrobial
resistance and ESBLs were higher in K. pneumoniae isolates than E. coli
isolates, and most K. pneumoniae isolates harbored simultaneously two or three
β-lactamases-encoding genes. Conclusion: High ESBL-producing rates among
K. pneumoniae and E. coli isolates and higher resistance rates to antibiotics
among ESBL compared to non-ESBL producing isolates necessitate
antimicrobial resistance surveillance and molecular characterization of ESBLsproducing
bacteria to achieve a specific treatment. |
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| ISSN: | 2345-5349 2345-5330 |