The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis
Abstract This study focused on the relationships among gut microbiota, plasma protein ratios, and tuberculosis. Given the unclear causal relationship between gut microbiota and tuberculosis and the scarcity of research on relevant plasma protein ratios in tuberculosis, Mendelian randomization analys...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87160-y |
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| author | Hanxin Wu Weijie Ma Liangyu Zhu Li Peng Xun Huang Lei Zhong Rui Yang Bingxue Li Weijiang Ma Li Gao Xinya Wu Jieqin Song Suyi Luo Fukai Bao Aihua Liu |
| author_facet | Hanxin Wu Weijie Ma Liangyu Zhu Li Peng Xun Huang Lei Zhong Rui Yang Bingxue Li Weijiang Ma Li Gao Xinya Wu Jieqin Song Suyi Luo Fukai Bao Aihua Liu |
| author_sort | Hanxin Wu |
| collection | DOAJ |
| description | Abstract This study focused on the relationships among gut microbiota, plasma protein ratios, and tuberculosis. Given the unclear causal relationship between gut microbiota and tuberculosis and the scarcity of research on relevant plasma protein ratios in tuberculosis, Mendelian randomization analysis (MR) was employed for in-depth exploration. By analyzing the GWAS data of individuals with European ancestry (the FinnGen dataset included 409,568 controls and 2613 cases), using the two-sample MR method, we focused on evaluating the impact of immunocyte-mediated gut microbiota on tuberculosis and the associations between 2821 plasma protein-to-protein ratios and tuberculosis. Particularly, the mediation effect was emphasized in the exploration. The results showed that 19 gut microbiotas were associated with tuberculosis. An7 indirectly affected tuberculosis through immunocyte (CD4 on CM CD4+), with a masking effect ratio of 0.008, demonstrating immune cells’ mediating role in the association between gut microbiota and tuberculosis. Meanwhile, the MR analysis revealed that 127 plasma protein-to-protein ratios were associated with tuberculosis. In conclusion, this study not only confirmed the impact of immunocyte-mediated gut microbiota on tuberculosis and clarified the mediating mechanism therein but also identified plasma protein-to-protein ratios related to tuberculosis, providing novel and valuable ideas for diagnosing and treating tuberculosis. |
| format | Article |
| id | doaj-art-57491e171cb14ce4b4fda9d3f4f52268 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-57491e171cb14ce4b4fda9d3f4f522682025-01-26T12:31:47ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-87160-yThe association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysisHanxin Wu0Weijie Ma1Liangyu Zhu2Li Peng3Xun Huang4Lei Zhong5Rui Yang6Bingxue Li7Weijiang Ma8Li Gao9Xinya Wu10Jieqin Song11Suyi Luo12Fukai Bao13Aihua Liu14Yunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityYunnan Provincial Key Laboratory of Children’s Major Diseases Research, School of Basic Medicine, Kunming Medical UniversityAbstract This study focused on the relationships among gut microbiota, plasma protein ratios, and tuberculosis. Given the unclear causal relationship between gut microbiota and tuberculosis and the scarcity of research on relevant plasma protein ratios in tuberculosis, Mendelian randomization analysis (MR) was employed for in-depth exploration. By analyzing the GWAS data of individuals with European ancestry (the FinnGen dataset included 409,568 controls and 2613 cases), using the two-sample MR method, we focused on evaluating the impact of immunocyte-mediated gut microbiota on tuberculosis and the associations between 2821 plasma protein-to-protein ratios and tuberculosis. Particularly, the mediation effect was emphasized in the exploration. The results showed that 19 gut microbiotas were associated with tuberculosis. An7 indirectly affected tuberculosis through immunocyte (CD4 on CM CD4+), with a masking effect ratio of 0.008, demonstrating immune cells’ mediating role in the association between gut microbiota and tuberculosis. Meanwhile, the MR analysis revealed that 127 plasma protein-to-protein ratios were associated with tuberculosis. In conclusion, this study not only confirmed the impact of immunocyte-mediated gut microbiota on tuberculosis and clarified the mediating mechanism therein but also identified plasma protein-to-protein ratios related to tuberculosis, providing novel and valuable ideas for diagnosing and treating tuberculosis.https://doi.org/10.1038/s41598-025-87160-yMendelian randomization (MR)TuberculosisGut microbiotaImmunocyteProtein-to-protein ratios |
| spellingShingle | Hanxin Wu Weijie Ma Liangyu Zhu Li Peng Xun Huang Lei Zhong Rui Yang Bingxue Li Weijiang Ma Li Gao Xinya Wu Jieqin Song Suyi Luo Fukai Bao Aihua Liu The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis Scientific Reports Mendelian randomization (MR) Tuberculosis Gut microbiota Immunocyte Protein-to-protein ratios |
| title | The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis |
| title_full | The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis |
| title_fullStr | The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis |
| title_full_unstemmed | The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis |
| title_short | The association of gut microbiota, immunocyte dynamics, and protein–protein ratios with tuberculosis susceptibility: a Mendelian randomization analysis |
| title_sort | association of gut microbiota immunocyte dynamics and protein protein ratios with tuberculosis susceptibility a mendelian randomization analysis |
| topic | Mendelian randomization (MR) Tuberculosis Gut microbiota Immunocyte Protein-to-protein ratios |
| url | https://doi.org/10.1038/s41598-025-87160-y |
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