Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate
Sepsis is a clinical condition causing tissue damage as a result of infection and an exaggerated immune response. Sepsis causes 11 million deaths annually, a third of which are associated with acute lung injury (ALI). Rapid and effective treatment is crucial to improve survival rates. <i>Punic...
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2025-01-01
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| author | Kazim Sahin Sena Sahin Aktura Ilkay Bahceci Tolga Mercantepe Levent Tumkaya Atilla Topcu Filiz Mercantepe Omer Faruk Duran Huseyin Avni Uydu Zihni Acar Yazici |
| author_facet | Kazim Sahin Sena Sahin Aktura Ilkay Bahceci Tolga Mercantepe Levent Tumkaya Atilla Topcu Filiz Mercantepe Omer Faruk Duran Huseyin Avni Uydu Zihni Acar Yazici |
| author_sort | Kazim Sahin |
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| description | Sepsis is a clinical condition causing tissue damage as a result of infection and an exaggerated immune response. Sepsis causes 11 million deaths annually, a third of which are associated with acute lung injury (ALI). Rapid and effective treatment is crucial to improve survival rates. <i>Punica granatum</i> (pomegranate) is rich in polyphenols and demonstrates strong antioxidant activity, while amifostine acts as a free radical scavenger. This study aimed to investigate the antioxidant and anti-inflammatory effects of <i>P. granatum</i> peel extract (PGPE) and amifostine in sepsis-related ALI. Experimental groups included Control, CLP (cecal ligation and puncture-induced sepsis), Amf (200 mg/kg amifostine, intraperitoneally), and PGPE250, and PGPE500 (250 and 500 mg/kg PGPE via oral gavage, respectively). Thiobarbituric acid reactive substances (TBARS), total thiol (TT), tumor necrosis factor-alpha (TNF-α) levels, and metalloproteinases 2 and 9 (MMP-2 and MMP-9) were assessed in the lung tissue. Biochemical analysis demonstrated that TBARS and TNF-α levels significantly decreased in both the PGPE and amifostine treatment groups compared to the CLP group, while TT levels showed notable improvement. Histopathological evaluation revealed reduced MMP-2 and MMP-9 immunopositivity in the PGPE250 and PGPE500 groups. These findings highlight the lung-protective properties of PGPE, underscoring its potential as a therapeutic agent for sepsis-induced acute lung injury. |
| format | Article |
| id | doaj-art-573fad26ed1e4c629e4f40f06362fd38 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-573fad26ed1e4c629e4f40f06362fd382025-01-24T13:38:42ZengMDPI AGLife2075-17292025-01-011517810.3390/life15010078Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus PomegranateKazim Sahin0Sena Sahin Aktura1Ilkay Bahceci2Tolga Mercantepe3Levent Tumkaya4Atilla Topcu5Filiz Mercantepe6Omer Faruk Duran7Huseyin Avni Uydu8Zihni Acar Yazici9Department of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Ondokuz Mayıs University, 55139 Samsun, TurkeyDepartment of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Endocrinology and Metabolism Diseases, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeyDepartment of Biochemistry, Faculty of Medicine, Samsun University, 55080 Samsun, TurkeyDepartment of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53020 Rize, TurkeySepsis is a clinical condition causing tissue damage as a result of infection and an exaggerated immune response. Sepsis causes 11 million deaths annually, a third of which are associated with acute lung injury (ALI). Rapid and effective treatment is crucial to improve survival rates. <i>Punica granatum</i> (pomegranate) is rich in polyphenols and demonstrates strong antioxidant activity, while amifostine acts as a free radical scavenger. This study aimed to investigate the antioxidant and anti-inflammatory effects of <i>P. granatum</i> peel extract (PGPE) and amifostine in sepsis-related ALI. Experimental groups included Control, CLP (cecal ligation and puncture-induced sepsis), Amf (200 mg/kg amifostine, intraperitoneally), and PGPE250, and PGPE500 (250 and 500 mg/kg PGPE via oral gavage, respectively). Thiobarbituric acid reactive substances (TBARS), total thiol (TT), tumor necrosis factor-alpha (TNF-α) levels, and metalloproteinases 2 and 9 (MMP-2 and MMP-9) were assessed in the lung tissue. Biochemical analysis demonstrated that TBARS and TNF-α levels significantly decreased in both the PGPE and amifostine treatment groups compared to the CLP group, while TT levels showed notable improvement. Histopathological evaluation revealed reduced MMP-2 and MMP-9 immunopositivity in the PGPE250 and PGPE500 groups. These findings highlight the lung-protective properties of PGPE, underscoring its potential as a therapeutic agent for sepsis-induced acute lung injury.https://www.mdpi.com/2075-1729/15/1/78amifostinececal-ligation puncturelung<i>Punica granatum</i> peelsepsis |
| spellingShingle | Kazim Sahin Sena Sahin Aktura Ilkay Bahceci Tolga Mercantepe Levent Tumkaya Atilla Topcu Filiz Mercantepe Omer Faruk Duran Huseyin Avni Uydu Zihni Acar Yazici Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate Life amifostine cecal-ligation puncture lung <i>Punica granatum</i> peel sepsis |
| title | Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate |
| title_full | Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate |
| title_fullStr | Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate |
| title_full_unstemmed | Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate |
| title_short | Is <i>Punica granatum</i> Efficient Against Sepsis? A Comparative Study of Amifostine Versus Pomegranate |
| title_sort | is i punica granatum i efficient against sepsis a comparative study of amifostine versus pomegranate |
| topic | amifostine cecal-ligation puncture lung <i>Punica granatum</i> peel sepsis |
| url | https://www.mdpi.com/2075-1729/15/1/78 |
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