Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways
Puerarin, a flavonoid compound present in the roots of radix <i>puerariae</i>, contributes to the development of tissues such as bone and nerve, but its role in skeletal muscle regeneration remains unclear. In this study, we employed C2C12 myoblasts and barium chloride (BaCl<sub>2&...
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2025-01-01
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| Online Access: | https://www.mdpi.com/2079-7737/14/1/102 |
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| author | Xiaofeng Fang Hangjia Xu Zhaoxin Fan Hongge Yang Yan Huang Lin Xu Yiwei Rong Wei Ma Liubao Pei Hongsheng Liang |
| author_facet | Xiaofeng Fang Hangjia Xu Zhaoxin Fan Hongge Yang Yan Huang Lin Xu Yiwei Rong Wei Ma Liubao Pei Hongsheng Liang |
| author_sort | Xiaofeng Fang |
| collection | DOAJ |
| description | Puerarin, a flavonoid compound present in the roots of radix <i>puerariae</i>, contributes to the development of tissues such as bone and nerve, but its role in skeletal muscle regeneration remains unclear. In this study, we employed C2C12 myoblasts and barium chloride (BaCl<sub>2</sub>)-based muscle injury models to investigate the effects of puerarin on myogenesis. Our study showed that puerarin stimulated the migration and differentiation of myoblasts in vitro. For the mechanism study, we found that puerarin’s influence on cell migration was associated with the activation of FAK signaling; additionally, puerarin induced myoblast differentiation by upregulating the PI3K/AKT pathway. We also found that puerarin treatment could improve muscle regeneration following muscle injury. Taken together, our data indicate that puerarin facilitated myogenesis by promoting migration and differentiation, which suggests puerarin as a new candidate drug for the treatment of muscle loss diseases. |
| format | Article |
| id | doaj-art-5739f513ed104dea8099244acb8f8f22 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-5739f513ed104dea8099244acb8f8f222025-01-24T13:23:39ZengMDPI AGBiology2079-77372025-01-0114110210.3390/biology14010102Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling PathwaysXiaofeng Fang0Hangjia Xu1Zhaoxin Fan2Hongge Yang3Yan Huang4Lin Xu5Yiwei Rong6Wei Ma7Liubao Pei8Hongsheng Liang9NHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaNHC Key Laboratory of Cell Transplantation, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaPuerarin, a flavonoid compound present in the roots of radix <i>puerariae</i>, contributes to the development of tissues such as bone and nerve, but its role in skeletal muscle regeneration remains unclear. In this study, we employed C2C12 myoblasts and barium chloride (BaCl<sub>2</sub>)-based muscle injury models to investigate the effects of puerarin on myogenesis. Our study showed that puerarin stimulated the migration and differentiation of myoblasts in vitro. For the mechanism study, we found that puerarin’s influence on cell migration was associated with the activation of FAK signaling; additionally, puerarin induced myoblast differentiation by upregulating the PI3K/AKT pathway. We also found that puerarin treatment could improve muscle regeneration following muscle injury. Taken together, our data indicate that puerarin facilitated myogenesis by promoting migration and differentiation, which suggests puerarin as a new candidate drug for the treatment of muscle loss diseases.https://www.mdpi.com/2079-7737/14/1/102puerarinmyoblastsmigrationdifferentiationmuscle regeneration |
| spellingShingle | Xiaofeng Fang Hangjia Xu Zhaoxin Fan Hongge Yang Yan Huang Lin Xu Yiwei Rong Wei Ma Liubao Pei Hongsheng Liang Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways Biology puerarin myoblasts migration differentiation muscle regeneration |
| title | Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways |
| title_full | Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways |
| title_fullStr | Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways |
| title_full_unstemmed | Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways |
| title_short | Puerarin Promotes the Migration and Differentiation of Myoblasts by Activating the FAK and PI3K/AKT Signaling Pathways |
| title_sort | puerarin promotes the migration and differentiation of myoblasts by activating the fak and pi3k akt signaling pathways |
| topic | puerarin myoblasts migration differentiation muscle regeneration |
| url | https://www.mdpi.com/2079-7737/14/1/102 |
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