H2-driven xylitol production in Cupriavidus necator H16
Abstract Background Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it c...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02615-7 |
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| author | Tytti Jämsä Nico J. Claassens Laura Salusjärvi Antti Nyyssölä |
| author_facet | Tytti Jämsä Nico J. Claassens Laura Salusjärvi Antti Nyyssölä |
| author_sort | Tytti Jämsä |
| collection | DOAJ |
| description | Abstract Background Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it can be efficiently produced from water using renewable electricity. In this article, resting cells of Cupriavidus necator H16 harboring a NAD-dependent hydrogenase were employed for cofactor recycling to reduce d-xylose to xylitol, a commonly used sweetener. To enable this bioconversion, d-xylose reductase from Scheffersomyces stipitis was heterologously expressed in C. necator. Results d-xylose reductase was successfully expressed in C. necator, enabling almost complete bioconversion of 30 g/L of d-xylose into xylitol. It was found that over 90% of the energy and protons derived from hydrogen were spent for the bioconversion, demonstrating the efficiency of the system. The highest xylitol productivity reached was 0.7 g/L/h. Additionally, the same chassis efficiently produced l-arabitol and d-ribitol from l-arabinose and d-ribose, respectively. Conclusions This study highlights the efficient utilization of renewable hydrogen as a reducing agent to power cofactor recycling. Hydrogen-oxidizing bacteria, such as C. necator, can be promising hosts for performing hydrogen-driven biocatalysis. |
| format | Article |
| id | doaj-art-572ffae2088941c5b2af7d14694a64f4 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-572ffae2088941c5b2af7d14694a64f42025-01-19T12:44:11ZengBMCMicrobial Cell Factories1475-28592024-12-0123111010.1186/s12934-024-02615-7H2-driven xylitol production in Cupriavidus necator H16Tytti Jämsä0Nico J. Claassens1Laura Salusjärvi2Antti Nyyssölä3VTT Technical Research Centre of Finland Ltd.Laboratory of Microbiology, Wageningen UniversityVTT Technical Research Centre of Finland Ltd.VTT Technical Research Centre of Finland Ltd.Abstract Background Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it can be efficiently produced from water using renewable electricity. In this article, resting cells of Cupriavidus necator H16 harboring a NAD-dependent hydrogenase were employed for cofactor recycling to reduce d-xylose to xylitol, a commonly used sweetener. To enable this bioconversion, d-xylose reductase from Scheffersomyces stipitis was heterologously expressed in C. necator. Results d-xylose reductase was successfully expressed in C. necator, enabling almost complete bioconversion of 30 g/L of d-xylose into xylitol. It was found that over 90% of the energy and protons derived from hydrogen were spent for the bioconversion, demonstrating the efficiency of the system. The highest xylitol productivity reached was 0.7 g/L/h. Additionally, the same chassis efficiently produced l-arabitol and d-ribitol from l-arabinose and d-ribose, respectively. Conclusions This study highlights the efficient utilization of renewable hydrogen as a reducing agent to power cofactor recycling. Hydrogen-oxidizing bacteria, such as C. necator, can be promising hosts for performing hydrogen-driven biocatalysis.https://doi.org/10.1186/s12934-024-02615-7BiotransformationCofactor recyclingCofactor regenerationRalstonia eutrophaCupriavidus necatorHydrogen-oxidizing bacteria |
| spellingShingle | Tytti Jämsä Nico J. Claassens Laura Salusjärvi Antti Nyyssölä H2-driven xylitol production in Cupriavidus necator H16 Microbial Cell Factories Biotransformation Cofactor recycling Cofactor regeneration Ralstonia eutropha Cupriavidus necator Hydrogen-oxidizing bacteria |
| title | H2-driven xylitol production in Cupriavidus necator H16 |
| title_full | H2-driven xylitol production in Cupriavidus necator H16 |
| title_fullStr | H2-driven xylitol production in Cupriavidus necator H16 |
| title_full_unstemmed | H2-driven xylitol production in Cupriavidus necator H16 |
| title_short | H2-driven xylitol production in Cupriavidus necator H16 |
| title_sort | h2 driven xylitol production in cupriavidus necator h16 |
| topic | Biotransformation Cofactor recycling Cofactor regeneration Ralstonia eutropha Cupriavidus necator Hydrogen-oxidizing bacteria |
| url | https://doi.org/10.1186/s12934-024-02615-7 |
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