Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii
ABSTRACT Sustainable chemical production from C1 gaseous substrates, such as syngas or CO2/H2, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbon source and electron acceptor, while reduced inorg...
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Wiley-VCH
2025-01-01
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| Series: | Engineering in Life Sciences |
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| Online Access: | https://doi.org/10.1002/elsc.202400076 |
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| author | Santiago T. Boto Kareem Gerges Bettina Bardl Miriam A. Rosenbaum |
| author_facet | Santiago T. Boto Kareem Gerges Bettina Bardl Miriam A. Rosenbaum |
| author_sort | Santiago T. Boto |
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| description | ABSTRACT Sustainable chemical production from C1 gaseous substrates, such as syngas or CO2/H2, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbon source and electron acceptor, while reduced inorganic species are used as the electron donor. Clostridium ljungdahlii, a model acetogen, is only capable of reducing CO2 to acetate and ethanol, with H2 as electron donor. In order to expand the product profile of this bacterium, five alcohol acetyltransferases (AATs) from yeast were heterologously expressed in C. ljungdahlii to evaluate its potential to produce ethyl acetate. When growing on CO2 and H2, up to 7.38 ± 0.43 mg/L of ethyl acetate were produced. Using fructose as the main carbon and energy source, up to 23.15 ± 1.28 mg/L of ethyl acetate were produced. Ethanol and fumarate supplementation were able to boost ethyl acetate titers (up to 37.51 ± 9.44 mg/L). Hence, ethyl acetate production was enabled in C. ljungdahlii at low titers, which could be explained by the high energetic cost of operation of AATs, and their shown promiscuity. However, we also show that this opens the door to more complex esterification reactions of higher added value biomolecules. |
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| institution | Kabale University |
| issn | 1618-0240 1618-2863 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
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| spelling | doaj-art-572c0b0dfe3140ac8972407d2ee758e82025-01-30T06:40:30ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632025-01-01251n/an/a10.1002/elsc.202400076Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahliiSantiago T. Boto0Kareem Gerges1Bettina Bardl2Miriam A. Rosenbaum3Bio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology – Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology – Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology – Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology – Hans‐Knöll‐Institute Jena GermanyABSTRACT Sustainable chemical production from C1 gaseous substrates, such as syngas or CO2/H2, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbon source and electron acceptor, while reduced inorganic species are used as the electron donor. Clostridium ljungdahlii, a model acetogen, is only capable of reducing CO2 to acetate and ethanol, with H2 as electron donor. In order to expand the product profile of this bacterium, five alcohol acetyltransferases (AATs) from yeast were heterologously expressed in C. ljungdahlii to evaluate its potential to produce ethyl acetate. When growing on CO2 and H2, up to 7.38 ± 0.43 mg/L of ethyl acetate were produced. Using fructose as the main carbon and energy source, up to 23.15 ± 1.28 mg/L of ethyl acetate were produced. Ethanol and fumarate supplementation were able to boost ethyl acetate titers (up to 37.51 ± 9.44 mg/L). Hence, ethyl acetate production was enabled in C. ljungdahlii at low titers, which could be explained by the high energetic cost of operation of AATs, and their shown promiscuity. However, we also show that this opens the door to more complex esterification reactions of higher added value biomolecules.https://doi.org/10.1002/elsc.202400076alcohol acetyltransferaseClostridium ljungdahliiethyl acetatefumarategas fermentation |
| spellingShingle | Santiago T. Boto Kareem Gerges Bettina Bardl Miriam A. Rosenbaum Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii Engineering in Life Sciences alcohol acetyltransferase Clostridium ljungdahlii ethyl acetate fumarate gas fermentation |
| title | Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii |
| title_full | Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii |
| title_fullStr | Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii |
| title_full_unstemmed | Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii |
| title_short | Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii |
| title_sort | evaluation of yeast alcohol acetyltransferases for ethyl acetate production in clostridium ljungdahlii |
| topic | alcohol acetyltransferase Clostridium ljungdahlii ethyl acetate fumarate gas fermentation |
| url | https://doi.org/10.1002/elsc.202400076 |
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