Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew
IntroductionCommon wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses a significant threat to grain yield and flour quality. The identification and utilization of broad-spectrum resistance genes against P...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1489013/full |
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| author | Bisheng Fu Bisheng Fu Bisheng Fu Zhixin Lin Lijuan Yan Qiaofeng Zhang Caiyun Liu Caiyun Liu Jin Cai Jin Cai Wei Guo Wei Guo Ying Liu Wenling Zhai Shuangjun Gong Feng Xu Jizhong Wu Jizhong Wu Jizhong Wu |
| author_facet | Bisheng Fu Bisheng Fu Bisheng Fu Zhixin Lin Lijuan Yan Qiaofeng Zhang Caiyun Liu Caiyun Liu Jin Cai Jin Cai Wei Guo Wei Guo Ying Liu Wenling Zhai Shuangjun Gong Feng Xu Jizhong Wu Jizhong Wu Jizhong Wu |
| author_sort | Bisheng Fu |
| collection | DOAJ |
| description | IntroductionCommon wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses a significant threat to grain yield and flour quality. The identification and utilization of broad-spectrum resistance genes against PM are essential for effective disease control.MethodsThe resistance spectrum test during the seedling stage and the identification of resistance during the adult stage were conducted to evaluate the wheat landrace Honghuamai (HHM). Five segregating populations were investigated to assess the inheritance of PM resistance in HHM. To map its PM resitance gene, bulked segregant analysis, molecular mapping and comparative genomic analysis were also used in the present study.ResultsHHM shows remarkable adult resistance in the field and is nearly immune to all 25 Bgt isolates used in seedling tests, making it an excellent source of PM resistance. PM resistance in HHM was determined by a single dominant gene, temporarily named PmHHM. It was then fine-mapped to an interval with a genetic distance of 0.0031 cM and a physical distance of 187.4 kb on chromosome 4AL of the Chinese Spring reference sequence v.2.1. Four genes were identified in the target region, three of which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative genomic analysis revealed presence/absence variations (PAVs) of the PmHHM locus among common wheat varieties.DiscussionThese closely linked molecular markers will not only benefit the cloning of the gene underlying PmHHM but also facilitate the efficient utilization of the gene in breeding programs. |
| format | Article |
| id | doaj-art-56e0e2ceac9f439496b48b2cd050abd0 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-56e0e2ceac9f439496b48b2cd050abd02025-02-06T07:10:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011510.3389/fpls.2024.14890131489013Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildewBisheng Fu0Bisheng Fu1Bisheng Fu2Zhixin Lin3Lijuan Yan4Qiaofeng Zhang5Caiyun Liu6Caiyun Liu7Jin Cai8Jin Cai9Wei Guo10Wei Guo11Ying Liu12Wenling Zhai13Shuangjun Gong14Feng Xu15Jizhong Wu16Jizhong Wu17Jizhong Wu18Institute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaZhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, ChinaJiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, ChinaCollege of Agriculture, Anhui Science and Technology University, Fengyang, Anhui, ChinaSchool of Life Sciences, Henan University, Kaifeng, Henan, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaZhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaZhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaZhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaInstitute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, ChinaCollege of Agriculture, Anhui Science and Technology University, Fengyang, Anhui, ChinaInstitute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaZhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, ChinaJiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, ChinaIntroductionCommon wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses a significant threat to grain yield and flour quality. The identification and utilization of broad-spectrum resistance genes against PM are essential for effective disease control.MethodsThe resistance spectrum test during the seedling stage and the identification of resistance during the adult stage were conducted to evaluate the wheat landrace Honghuamai (HHM). Five segregating populations were investigated to assess the inheritance of PM resistance in HHM. To map its PM resitance gene, bulked segregant analysis, molecular mapping and comparative genomic analysis were also used in the present study.ResultsHHM shows remarkable adult resistance in the field and is nearly immune to all 25 Bgt isolates used in seedling tests, making it an excellent source of PM resistance. PM resistance in HHM was determined by a single dominant gene, temporarily named PmHHM. It was then fine-mapped to an interval with a genetic distance of 0.0031 cM and a physical distance of 187.4 kb on chromosome 4AL of the Chinese Spring reference sequence v.2.1. Four genes were identified in the target region, three of which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative genomic analysis revealed presence/absence variations (PAVs) of the PmHHM locus among common wheat varieties.DiscussionThese closely linked molecular markers will not only benefit the cloning of the gene underlying PmHHM but also facilitate the efficient utilization of the gene in breeding programs.https://www.frontiersin.org/articles/10.3389/fpls.2024.1489013/fullBlumeria graminisgenetic markersresistance breedingTriticum aestivum L.PmHHM |
| spellingShingle | Bisheng Fu Bisheng Fu Bisheng Fu Zhixin Lin Lijuan Yan Qiaofeng Zhang Caiyun Liu Caiyun Liu Jin Cai Jin Cai Wei Guo Wei Guo Ying Liu Wenling Zhai Shuangjun Gong Feng Xu Jizhong Wu Jizhong Wu Jizhong Wu Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew Frontiers in Plant Science Blumeria graminis genetic markers resistance breeding Triticum aestivum L. PmHHM |
| title | Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| title_full | Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| title_fullStr | Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| title_full_unstemmed | Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| title_short | Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| title_sort | fine mapping of pmhhm a broad spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew |
| topic | Blumeria graminis genetic markers resistance breeding Triticum aestivum L. PmHHM |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1489013/full |
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