Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species
South American Akodontini rodents are characterized by a large number of chromosome rearrangements. Among them, the genus Akodon has been extensively analyzed with classical and molecular cytogenetics, which allowed the identification of a large number of intra- and interspecific chromosomal variati...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/5935380 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559416190173184 |
|---|---|
| author | Naiara Pereira Araújo Gustavo Campos Silva Kuhn Flávia Nunes Vieira Thaís Queiroz Morcatty Adriano Pereira Paglia Marta Svartman |
| author_facet | Naiara Pereira Araújo Gustavo Campos Silva Kuhn Flávia Nunes Vieira Thaís Queiroz Morcatty Adriano Pereira Paglia Marta Svartman |
| author_sort | Naiara Pereira Araújo |
| collection | DOAJ |
| description | South American Akodontini rodents are characterized by a large number of chromosome rearrangements. Among them, the genus Akodon has been extensively analyzed with classical and molecular cytogenetics, which allowed the identification of a large number of intra- and interspecific chromosomal variation due to Robertsonian rearrangements, pericentric inversions, and heterochromatin additions/deletions. In order to shed some light on the cause of these rearrangements, we comparatively analyzed the karyotypes of three Akodontini species, Akodon cursor (2n = 14, FN = 19), A. montensis (2n = 24, FN = 42), and Necromys lasiurus (2n = 34, FN = 34), after GTG- and CBG-banding. The karyotypes differed by Robertsonian rearrangements, pericentric inversions, centromere repositioning, and heterochromatin variation. Genome comparisons were performed through interspecific fluorescent in situ hybridization (FISH) with total genomic DNAs of each species as probes (GISH). Our results revealed considerable conservation of the euchromatic portions among the three karyotypes suggesting that they mostly differ in their heterochromatic regions. FISH was also performed to assess the distribution of telomeric sequences, long and short interspersed repetitive elements (LINE-1 and B1 SINE) and of the endogenous retrovirus mysTR in the genomes of the three species. The results led us to infer that transposable elements have played an important role in the enormous chromosome variation found in Akodontini. |
| format | Article |
| id | doaj-art-56d7afea2161419a9cfe0b25bfcbbf00 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-56d7afea2161419a9cfe0b25bfcbbf002025-02-03T01:30:11ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/59353805935380Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini SpeciesNaiara Pereira Araújo0Gustavo Campos Silva Kuhn1Flávia Nunes Vieira2Thaís Queiroz Morcatty3Adriano Pereira Paglia4Marta Svartman5Laboratório de Citogenômica Evolutiva, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilLaboratório de Citogenômica Evolutiva, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilLaboratório de Ecologia e Conservação, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilLaboratório de Ecologia e Conservação, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilLaboratório de Ecologia e Conservação, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilLaboratório de Citogenômica Evolutiva, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627-Pampulha, 31270-901 Belo Horizonte, MG, BrazilSouth American Akodontini rodents are characterized by a large number of chromosome rearrangements. Among them, the genus Akodon has been extensively analyzed with classical and molecular cytogenetics, which allowed the identification of a large number of intra- and interspecific chromosomal variation due to Robertsonian rearrangements, pericentric inversions, and heterochromatin additions/deletions. In order to shed some light on the cause of these rearrangements, we comparatively analyzed the karyotypes of three Akodontini species, Akodon cursor (2n = 14, FN = 19), A. montensis (2n = 24, FN = 42), and Necromys lasiurus (2n = 34, FN = 34), after GTG- and CBG-banding. The karyotypes differed by Robertsonian rearrangements, pericentric inversions, centromere repositioning, and heterochromatin variation. Genome comparisons were performed through interspecific fluorescent in situ hybridization (FISH) with total genomic DNAs of each species as probes (GISH). Our results revealed considerable conservation of the euchromatic portions among the three karyotypes suggesting that they mostly differ in their heterochromatic regions. FISH was also performed to assess the distribution of telomeric sequences, long and short interspersed repetitive elements (LINE-1 and B1 SINE) and of the endogenous retrovirus mysTR in the genomes of the three species. The results led us to infer that transposable elements have played an important role in the enormous chromosome variation found in Akodontini.http://dx.doi.org/10.1155/2017/5935380 |
| spellingShingle | Naiara Pereira Araújo Gustavo Campos Silva Kuhn Flávia Nunes Vieira Thaís Queiroz Morcatty Adriano Pereira Paglia Marta Svartman Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species International Journal of Genomics |
| title | Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species |
| title_full | Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species |
| title_fullStr | Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species |
| title_full_unstemmed | Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species |
| title_short | Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species |
| title_sort | comparative genomic in situ hybridization and the possible role of retroelements in the karyotypic evolution of three akodontini species |
| url | http://dx.doi.org/10.1155/2017/5935380 |
| work_keys_str_mv | AT naiarapereiraaraujo comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies AT gustavocampossilvakuhn comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies AT flavianunesvieira comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies AT thaisqueirozmorcatty comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies AT adrianopereirapaglia comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies AT martasvartman comparativegenomicinsituhybridizationandthepossibleroleofretroelementsinthekaryotypicevolutionofthreeakodontinispecies |