Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p
Background. Respiratory syncytial virus (RSV) infection can regulate the expression of a wide range of noncoding microRNAs (miRNAs), in which mir-19a-3p can participate in airway inflammatory response by regulating 5-lipoxygenase (5-LO) pathway. RSV nonstructural protein (NS) 1 is involved in the ai...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/4086710 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553680434364416 |
|---|---|
| author | Mei Zheng Panpan Fan Pu Yang Junwen Zheng Dongchi Zhao |
| author_facet | Mei Zheng Panpan Fan Pu Yang Junwen Zheng Dongchi Zhao |
| author_sort | Mei Zheng |
| collection | DOAJ |
| description | Background. Respiratory syncytial virus (RSV) infection can regulate the expression of a wide range of noncoding microRNAs (miRNAs), in which mir-19a-3p can participate in airway inflammatory response by regulating 5-lipoxygenase (5-LO) pathway. RSV nonstructural protein (NS) 1 is involved in the airway hyperresponsiveness during RSV infection. Methods. The expression levels of miR-19a-3p and inflammatory signaling-related indicators were detected using quantitative real-time PCR and western blot analyses on the A549 cells transfected with NS1 expression plasmids (pNS1). The 5-LO-mediated inflammatory signaling pathway was assessed when the miR-19a-3p or 5-LO was inhibited. Results. The immunofluorescence analysis showed that the plasmid-mediated NS1 protein was observed in both the cytoplasm and nucleus. The expression level of miR-19a-3p was significantly upregulated in the pNS1 or RSV-treated cells, which was reversed by the NS1 small interfering RNA. In addition, pNS1 also upregulated the expression of 5-LO, interleukin-5 (IL-5), and leukotriene B4 (LTB4), which was also significantly inhibited by the miR-19a-3p antagonists. The 5-LO inhibitor MK886 prevented the increase in the expression level of IL-5 induced by pNS1. Conclusions. These results suggested that the RSV NS1 might play an important role in the pathogenesis of RSV by activating the 5-LO and subsequent inflammatory cytokines through miR-19a-3p. |
| format | Article |
| id | doaj-art-563d8cb33f454131b4c9d3ac70cbeea1 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-563d8cb33f454131b4c9d3ac70cbeea12025-02-03T05:53:30ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/4086710Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3pMei Zheng0Panpan Fan1Pu Yang2Junwen Zheng3Dongchi Zhao4Department of PediatricsDepartment of PediatricsDepartment of PediatricsDepartment of PediatricsDepartment of PediatricsBackground. Respiratory syncytial virus (RSV) infection can regulate the expression of a wide range of noncoding microRNAs (miRNAs), in which mir-19a-3p can participate in airway inflammatory response by regulating 5-lipoxygenase (5-LO) pathway. RSV nonstructural protein (NS) 1 is involved in the airway hyperresponsiveness during RSV infection. Methods. The expression levels of miR-19a-3p and inflammatory signaling-related indicators were detected using quantitative real-time PCR and western blot analyses on the A549 cells transfected with NS1 expression plasmids (pNS1). The 5-LO-mediated inflammatory signaling pathway was assessed when the miR-19a-3p or 5-LO was inhibited. Results. The immunofluorescence analysis showed that the plasmid-mediated NS1 protein was observed in both the cytoplasm and nucleus. The expression level of miR-19a-3p was significantly upregulated in the pNS1 or RSV-treated cells, which was reversed by the NS1 small interfering RNA. In addition, pNS1 also upregulated the expression of 5-LO, interleukin-5 (IL-5), and leukotriene B4 (LTB4), which was also significantly inhibited by the miR-19a-3p antagonists. The 5-LO inhibitor MK886 prevented the increase in the expression level of IL-5 induced by pNS1. Conclusions. These results suggested that the RSV NS1 might play an important role in the pathogenesis of RSV by activating the 5-LO and subsequent inflammatory cytokines through miR-19a-3p.http://dx.doi.org/10.1155/2022/4086710 |
| spellingShingle | Mei Zheng Panpan Fan Pu Yang Junwen Zheng Dongchi Zhao Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p Journal of Immunology Research |
| title | Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p |
| title_full | Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p |
| title_fullStr | Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p |
| title_full_unstemmed | Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p |
| title_short | Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p |
| title_sort | respiratory syncytial virus nonstructural protein 1 promotes 5 lipoxygenase via mir 19a 3p |
| url | http://dx.doi.org/10.1155/2022/4086710 |
| work_keys_str_mv | AT meizheng respiratorysyncytialvirusnonstructuralprotein1promotes5lipoxygenaseviamir19a3p AT panpanfan respiratorysyncytialvirusnonstructuralprotein1promotes5lipoxygenaseviamir19a3p AT puyang respiratorysyncytialvirusnonstructuralprotein1promotes5lipoxygenaseviamir19a3p AT junwenzheng respiratorysyncytialvirusnonstructuralprotein1promotes5lipoxygenaseviamir19a3p AT dongchizhao respiratorysyncytialvirusnonstructuralprotein1promotes5lipoxygenaseviamir19a3p |