Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice
<i>Lutjanus erythropterus</i> protein (Lep) exhibits anti-inflammatory effects, but its antidepressant activity is unknown. This study used a 44-day chronic unpredictable mild stress (CUMS) model to determine whether Lep has a beneficial effect through the gut–brain axis in 3-week-old ma...
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2025-01-01
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| author | Jinjin Luo Chen Wang Weichang Ye Ruiyang He Ling Huang Zhijia Fang Qi Deng Mei Qiu Lijun Sun Ravi Gooneratne |
| author_facet | Jinjin Luo Chen Wang Weichang Ye Ruiyang He Ling Huang Zhijia Fang Qi Deng Mei Qiu Lijun Sun Ravi Gooneratne |
| author_sort | Jinjin Luo |
| collection | DOAJ |
| description | <i>Lutjanus erythropterus</i> protein (Lep) exhibits anti-inflammatory effects, but its antidepressant activity is unknown. This study used a 44-day chronic unpredictable mild stress (CUMS) model to determine whether Lep has a beneficial effect through the gut–brain axis in 3-week-old male C57BL/6 mice. Gavaging with Lep solution alleviated the depression-like behavior and anxiety symptoms in CUMS growing mice. Administration of Lep decreased serum IL-1β, IL-2, IL-6, and TNF-α levels and restored colonic mucosal damage. In addition, Lep improved the disturbance of 5-hydroxytryptamine (5-HT) secretion in the gut–brain axis. Pearson analysis revealed that gut short-chain fatty acid (SCFAs) concentration significantly (<i>p</i> < 0.05) correlated with mucosal damage scores and the depression-like behavior index. Lep was able to prevent the gut SCFA enrichment. Lep upregulated gut <i>Muribaculaceae</i> and downregulated SCFA-producing bacteria by replenishing deficient amino acid (AA) (tryptophan, alanine, aspartate, glutamate) and decreased (<i>p</i> < 0.01) the gene abundance of the AA metabolism pathway of SCFA-producing bacteria, thereby preventing gut SCFA enrichment and alleviating associated depression-like behavior. These findings indicate that Lep could attenuate depression in CUMS juvenile mice via the gut microbiota-SCFA–brain axis. |
| format | Article |
| id | doaj-art-561843e9dd3f4013a8355eb3202a560c |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Foods |
| spelling | doaj-art-561843e9dd3f4013a8355eb3202a560c2025-01-24T13:33:18ZengMDPI AGFoods2304-81582025-01-0114233010.3390/foods14020330Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile MiceJinjin Luo0Chen Wang1Weichang Ye2Ruiyang He3Ling Huang4Zhijia Fang5Qi Deng6Mei Qiu7Lijun Sun8Ravi Gooneratne9College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, P.O. Box 85084, Lincoln 7647, New Zealand<i>Lutjanus erythropterus</i> protein (Lep) exhibits anti-inflammatory effects, but its antidepressant activity is unknown. This study used a 44-day chronic unpredictable mild stress (CUMS) model to determine whether Lep has a beneficial effect through the gut–brain axis in 3-week-old male C57BL/6 mice. Gavaging with Lep solution alleviated the depression-like behavior and anxiety symptoms in CUMS growing mice. Administration of Lep decreased serum IL-1β, IL-2, IL-6, and TNF-α levels and restored colonic mucosal damage. In addition, Lep improved the disturbance of 5-hydroxytryptamine (5-HT) secretion in the gut–brain axis. Pearson analysis revealed that gut short-chain fatty acid (SCFAs) concentration significantly (<i>p</i> < 0.05) correlated with mucosal damage scores and the depression-like behavior index. Lep was able to prevent the gut SCFA enrichment. Lep upregulated gut <i>Muribaculaceae</i> and downregulated SCFA-producing bacteria by replenishing deficient amino acid (AA) (tryptophan, alanine, aspartate, glutamate) and decreased (<i>p</i> < 0.01) the gene abundance of the AA metabolism pathway of SCFA-producing bacteria, thereby preventing gut SCFA enrichment and alleviating associated depression-like behavior. These findings indicate that Lep could attenuate depression in CUMS juvenile mice via the gut microbiota-SCFA–brain axis.https://www.mdpi.com/2304-8158/14/2/330adolescentsamino aciddepressiongut microbiota<i>Lutjanus erythropterus</i> proteinshort-chain fatty acids |
| spellingShingle | Jinjin Luo Chen Wang Weichang Ye Ruiyang He Ling Huang Zhijia Fang Qi Deng Mei Qiu Lijun Sun Ravi Gooneratne Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice Foods adolescents amino acid depression gut microbiota <i>Lutjanus erythropterus</i> protein short-chain fatty acids |
| title | Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice |
| title_full | Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice |
| title_fullStr | Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice |
| title_full_unstemmed | Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice |
| title_short | Effects of <i>Lutjanus erythropterus</i> Protein on Depression-like Behavior and Gut Microbiota in Stressed Juvenile Mice |
| title_sort | effects of i lutjanus erythropterus i protein on depression like behavior and gut microbiota in stressed juvenile mice |
| topic | adolescents amino acid depression gut microbiota <i>Lutjanus erythropterus</i> protein short-chain fatty acids |
| url | https://www.mdpi.com/2304-8158/14/2/330 |
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