gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism
Background. gp91phox, the catalytic core of NADPH oxidase (NOX) and biomarker of NOX activation, has been recently recognized as a parameter of systemic oxidative stress in several studies. Subclinical hypothyroidism (SH) is characteristic of elevated level of serum thyroid stimulating hormone (TSH)...
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| Format: | Article |
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Wiley
2020-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2020/3161730 |
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| author | Xiaochun Ma Furong Wang Xiaowen Zhen Lifang Zhao Li Fang Zhenfang Dong Wenbin Chen Xiaoming Zhou |
| author_facet | Xiaochun Ma Furong Wang Xiaowen Zhen Lifang Zhao Li Fang Zhenfang Dong Wenbin Chen Xiaoming Zhou |
| author_sort | Xiaochun Ma |
| collection | DOAJ |
| description | Background. gp91phox, the catalytic core of NADPH oxidase (NOX) and biomarker of NOX activation, has been recently recognized as a parameter of systemic oxidative stress in several studies. Subclinical hypothyroidism (SH) is characteristic of elevated level of serum thyroid stimulating hormone (TSH) and is frequently accompanied with cholesterolemia. In this study, the levels of serum soluble gp91phox were measured to assess the oxidative stress in patients with SH. And the relationship among gp91phox, low-density lipoprotein-C (LDL-C), and TSH was also investigated. Methods. A total of 51 subjects were enrolled and categorized into four groups: the healthy controls subjects (n = 13), controls with high level of LDL-C alone (n = 12), SH with normal level of LDL-C (n = 11), and SH with high level of LDL-C (n = 15). The related clinical and laboratory data were collected for statistical analysis. All the patients were newly diagnosed and did not take any medication. The information of lipid profile and thyroid function was extracted, and the concentrations of gp91phox were obtained with ELISA. Results. The levels of serum soluble gp91phox evidently increased in the patients with SH with a high level of LDL-C (81.52 ± 37.00 ug/mL) as compared to the healthy controls (54.98 ± 1.83ug/mL, p<0.001), controls with high level of LDL-C (61.21 ± 4.48 ug/mL, p=0.038) and SH with a normal level of LDL-C (62.82 ± 11.67ug/mL, p=0.027). Additionally, the levels of gp91phox showed a significant positive correlation with both the levels of LDL-C (r = 0.595, p<0.001) and TSH (r = 0.346, p=0.013) by the Spearman correlation analyses. The correlation remained significant even when the effect of another factor was controlled (TSH: when the effect of LDL-C was controlled, r = 0.453, p=0.001; LDL-C: when the effect of TSH was controlled, r = 0.291, p=0.040). The main effect analysis showed an independent main effect of either LDL-C (p = 0.041) or TSH (p=0.022) on gp91phox without interaction (p=0.299). Conclusions. Our work demonstrated that the levels of gp91phox, a novel biomarker for measuring the oxidative stress, were significantly elevated in the patients with SH. And LDL-C and TSH were both independent predictors of gp91phox. Abbreviations. BMI : Body mass index; TC : Total cholesterol; LDL-C : Low-density lipoprotein cholesterol; HDL-C : High-density lipoprotein cholesterol; TG : Triglyceride; FBG : Fasting blood glucose; FT3 : Free triiodothyronine; FT4 : Free thyroxine; TSH: Thyroid stimulating hormone; SBP : Systolic blood pressure; DBP : Diastolic blood pressure; SD : Standard deviation; LSD: Least significant difference. |
| format | Article |
| id | doaj-art-55fa856d8b684f7198d97e9a33e0fe55 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2020-01-01 |
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| series | International Journal of Endocrinology |
| spelling | doaj-art-55fa856d8b684f7198d97e9a33e0fe552025-02-03T06:46:36ZengWileyInternational Journal of Endocrinology1687-83371687-83452020-01-01202010.1155/2020/31617303161730gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical HypothyroidismXiaochun Ma0Furong Wang1Xiaowen Zhen2Lifang Zhao3Li Fang4Zhenfang Dong5Wenbin Chen6Xiaoming Zhou7Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaDepartment of Pharmacology, College of TCM, Shandong University of Traditional Chinese Medicine, Jinan, 250355 Shandong, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaDepartment of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaScientific Center, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021 Shandong, ChinaBackground. gp91phox, the catalytic core of NADPH oxidase (NOX) and biomarker of NOX activation, has been recently recognized as a parameter of systemic oxidative stress in several studies. Subclinical hypothyroidism (SH) is characteristic of elevated level of serum thyroid stimulating hormone (TSH) and is frequently accompanied with cholesterolemia. In this study, the levels of serum soluble gp91phox were measured to assess the oxidative stress in patients with SH. And the relationship among gp91phox, low-density lipoprotein-C (LDL-C), and TSH was also investigated. Methods. A total of 51 subjects were enrolled and categorized into four groups: the healthy controls subjects (n = 13), controls with high level of LDL-C alone (n = 12), SH with normal level of LDL-C (n = 11), and SH with high level of LDL-C (n = 15). The related clinical and laboratory data were collected for statistical analysis. All the patients were newly diagnosed and did not take any medication. The information of lipid profile and thyroid function was extracted, and the concentrations of gp91phox were obtained with ELISA. Results. The levels of serum soluble gp91phox evidently increased in the patients with SH with a high level of LDL-C (81.52 ± 37.00 ug/mL) as compared to the healthy controls (54.98 ± 1.83ug/mL, p<0.001), controls with high level of LDL-C (61.21 ± 4.48 ug/mL, p=0.038) and SH with a normal level of LDL-C (62.82 ± 11.67ug/mL, p=0.027). Additionally, the levels of gp91phox showed a significant positive correlation with both the levels of LDL-C (r = 0.595, p<0.001) and TSH (r = 0.346, p=0.013) by the Spearman correlation analyses. The correlation remained significant even when the effect of another factor was controlled (TSH: when the effect of LDL-C was controlled, r = 0.453, p=0.001; LDL-C: when the effect of TSH was controlled, r = 0.291, p=0.040). The main effect analysis showed an independent main effect of either LDL-C (p = 0.041) or TSH (p=0.022) on gp91phox without interaction (p=0.299). Conclusions. Our work demonstrated that the levels of gp91phox, a novel biomarker for measuring the oxidative stress, were significantly elevated in the patients with SH. And LDL-C and TSH were both independent predictors of gp91phox. Abbreviations. BMI : Body mass index; TC : Total cholesterol; LDL-C : Low-density lipoprotein cholesterol; HDL-C : High-density lipoprotein cholesterol; TG : Triglyceride; FBG : Fasting blood glucose; FT3 : Free triiodothyronine; FT4 : Free thyroxine; TSH: Thyroid stimulating hormone; SBP : Systolic blood pressure; DBP : Diastolic blood pressure; SD : Standard deviation; LSD: Least significant difference.http://dx.doi.org/10.1155/2020/3161730 |
| spellingShingle | Xiaochun Ma Furong Wang Xiaowen Zhen Lifang Zhao Li Fang Zhenfang Dong Wenbin Chen Xiaoming Zhou gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism International Journal of Endocrinology |
| title | gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism |
| title_full | gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism |
| title_fullStr | gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism |
| title_full_unstemmed | gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism |
| title_short | gp91phox, a Novel Biomarker Evaluating Oxidative Stress, Is Elevated in Subclinical Hypothyroidism |
| title_sort | gp91phox a novel biomarker evaluating oxidative stress is elevated in subclinical hypothyroidism |
| url | http://dx.doi.org/10.1155/2020/3161730 |
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