Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism
Autism spectrum disorders (ASDs) are a complex group of neurodevelopment disorders steadily rising in frequency and treatment refractory, where the search for biological markers is of paramount importance. Although red blood cells (RBCs) membrane lipidomics and rheological variables have been report...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/432616 |
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| author | Lucia Ciccoli Claudio De Felice Eugenio Paccagnini Silvia Leoncini Alessandra Pecorelli Cinzia Signorini Giuseppe Belmonte Roberto Guerranti Alessio Cortelazzo Mariangela Gentile Gloria Zollo Thierry Durand Giuseppe Valacchi Marcello Rossi Joussef Hayek |
| author_facet | Lucia Ciccoli Claudio De Felice Eugenio Paccagnini Silvia Leoncini Alessandra Pecorelli Cinzia Signorini Giuseppe Belmonte Roberto Guerranti Alessio Cortelazzo Mariangela Gentile Gloria Zollo Thierry Durand Giuseppe Valacchi Marcello Rossi Joussef Hayek |
| author_sort | Lucia Ciccoli |
| collection | DOAJ |
| description | Autism spectrum disorders (ASDs) are a complex group of neurodevelopment disorders steadily rising in frequency and treatment refractory, where the search for biological markers is of paramount importance. Although red blood cells (RBCs) membrane lipidomics and rheological variables have been reported to be altered, with some suggestions indicating an increased lipid peroxidation in the erythrocyte membrane, to date no information exists on how the oxidative membrane damage may affect cytoskeletal membrane proteins and, ultimately, RBCs shape in autism. Here, we investigated RBC morphology by scanning electron microscopy in patients with classical autism, that is, the predominant ASDs phenotype (age range: 6–26 years), nonautistic neurodevelopmental disorders (i.e., “positive controls”), and healthy controls (i.e., “negative controls”). A high percentage of altered RBCs shapes, predominantly elliptocytes, was observed in autistic patients, but not in both control groups. The RBCs altered morphology in autistic subjects was related to increased erythrocyte membrane F2-isoprostanes and 4-hydroxynonenal protein adducts. In addition, an oxidative damage of the erythrocyte membrane β-actin protein was evidenced. Therefore, the combination of erythrocyte shape abnormalities, erythrocyte membrane oxidative damage, and β-actin alterations constitutes a previously unrecognized triad in classical autism and provides new biological markers in the diagnostic workup of ASDs. |
| format | Article |
| id | doaj-art-55af2528a0d8448c8be64939b8e060dc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-55af2528a0d8448c8be64939b8e060dc2025-02-03T01:21:11ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/432616432616Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical AutismLucia Ciccoli0Claudio De Felice1Eugenio Paccagnini2Silvia Leoncini3Alessandra Pecorelli4Cinzia Signorini5Giuseppe Belmonte6Roberto Guerranti7Alessio Cortelazzo8Mariangela Gentile9Gloria Zollo10Thierry Durand11Giuseppe Valacchi12Marcello Rossi13Joussef Hayek14Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, ItalyNeonatal Intensive Care Unit, University Hospital, Azienda Ospedaliera Universitaria Senese (AOUS), Viale M. Bracci 16, 53100 Siena, ItalyDepartment of Life Sciences, University of Siena, Via A. Moro 2, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, ItalyMedicine, Surgery and Neurosciences Department, University of Siena, Viale M. Bracci 16, 53100 Siena, ItalyClinical Pathology Laboratory Unit, University Hospital, AOUS, Viale M. Bracci 16, 53100 Siena, ItalyChild Neuropsychiatry Unit, University Hospital, AOUS, Viale M. Bracci 16, 53100 Siena, ItalyDepartment of Life Sciences, University of Siena, Via A. Moro 2, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, ItalyInstitut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS/UM1/UM2, BP 14491 34093, Montpellier, Cedex 5, FranceLife Science and Biotechnologies, University of Ferrara, Via Borsari 46, 44100 Ferrara, ItalyRespiratory Pathophysiology and Rehabilitation Unit, University Hospital, AOUS, Viale M. Bracci 16, 53100 Siena, ItalyChild Neuropsychiatry Unit, University Hospital, AOUS, Viale M. Bracci 16, 53100 Siena, ItalyAutism spectrum disorders (ASDs) are a complex group of neurodevelopment disorders steadily rising in frequency and treatment refractory, where the search for biological markers is of paramount importance. Although red blood cells (RBCs) membrane lipidomics and rheological variables have been reported to be altered, with some suggestions indicating an increased lipid peroxidation in the erythrocyte membrane, to date no information exists on how the oxidative membrane damage may affect cytoskeletal membrane proteins and, ultimately, RBCs shape in autism. Here, we investigated RBC morphology by scanning electron microscopy in patients with classical autism, that is, the predominant ASDs phenotype (age range: 6–26 years), nonautistic neurodevelopmental disorders (i.e., “positive controls”), and healthy controls (i.e., “negative controls”). A high percentage of altered RBCs shapes, predominantly elliptocytes, was observed in autistic patients, but not in both control groups. The RBCs altered morphology in autistic subjects was related to increased erythrocyte membrane F2-isoprostanes and 4-hydroxynonenal protein adducts. In addition, an oxidative damage of the erythrocyte membrane β-actin protein was evidenced. Therefore, the combination of erythrocyte shape abnormalities, erythrocyte membrane oxidative damage, and β-actin alterations constitutes a previously unrecognized triad in classical autism and provides new biological markers in the diagnostic workup of ASDs.http://dx.doi.org/10.1155/2013/432616 |
| spellingShingle | Lucia Ciccoli Claudio De Felice Eugenio Paccagnini Silvia Leoncini Alessandra Pecorelli Cinzia Signorini Giuseppe Belmonte Roberto Guerranti Alessio Cortelazzo Mariangela Gentile Gloria Zollo Thierry Durand Giuseppe Valacchi Marcello Rossi Joussef Hayek Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism Mediators of Inflammation |
| title | Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism |
| title_full | Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism |
| title_fullStr | Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism |
| title_full_unstemmed | Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism |
| title_short | Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism |
| title_sort | erythrocyte shape abnormalities membrane oxidative damage and β actin alterations an unrecognized triad in classical autism |
| url | http://dx.doi.org/10.1155/2013/432616 |
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