Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC
Matrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in P. acacia. The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/9598606 |
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| author | Mashail N. AlZain Rashed N. Herqash Abdulaziz N. Almoqbil Omer Mohammed Almarfadi Mansour N. Ibrahim Riaz Ullah Omar M. Noman |
| author_facet | Mashail N. AlZain Rashed N. Herqash Abdulaziz N. Almoqbil Omer Mohammed Almarfadi Mansour N. Ibrahim Riaz Ullah Omar M. Noman |
| author_sort | Mashail N. AlZain |
| collection | DOAJ |
| description | Matrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in P. acacia. The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to the dispersant, nature of dispersant, and the nature of elution solvent and its volume) were examined and optimized. These MSPD optimized parameters regulated are as follows: 8 mL of methanol was utilized as elution solvent, silica gel/sample mass ration was selected to be 2 : 1, and dispersing sorbent was silica gel. The technique retrievals were regulated to be “from 96.87 to 100.54%” and the RSDs from 1.24 to 4.45%. The product of extract obtained by MSPD method was larger than that of other methods, i.e., sonication extraction or traditional reflux with lessened necessities on time, sample, and solvent. |
| format | Article |
| id | doaj-art-5590145c08834b63ad1d94ef317120d2 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-5590145c08834b63ad1d94ef317120d22025-02-03T01:04:08ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/95986069598606Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLCMashail N. AlZain0Rashed N. Herqash1Abdulaziz N. Almoqbil2Omer Mohammed Almarfadi3Mansour N. Ibrahim4Riaz Ullah5Omar M. Noman6Department of Biology, College of Sciences, Princess Nourah Bint Abdulrahman University, Riyadh 11451, Saudi ArabiaMedicinal, Aromatic, and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaMedicinal, Aromatic, and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Agricultural Engineering, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaMedicinal, Aromatic, and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaMedicinal, Aromatic, and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaMatrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in P. acacia. The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to the dispersant, nature of dispersant, and the nature of elution solvent and its volume) were examined and optimized. These MSPD optimized parameters regulated are as follows: 8 mL of methanol was utilized as elution solvent, silica gel/sample mass ration was selected to be 2 : 1, and dispersing sorbent was silica gel. The technique retrievals were regulated to be “from 96.87 to 100.54%” and the RSDs from 1.24 to 4.45%. The product of extract obtained by MSPD method was larger than that of other methods, i.e., sonication extraction or traditional reflux with lessened necessities on time, sample, and solvent.http://dx.doi.org/10.1155/2020/9598606 |
| spellingShingle | Mashail N. AlZain Rashed N. Herqash Abdulaziz N. Almoqbil Omer Mohammed Almarfadi Mansour N. Ibrahim Riaz Ullah Omar M. Noman Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC Journal of Analytical Methods in Chemistry |
| title | Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC |
| title_full | Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC |
| title_fullStr | Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC |
| title_full_unstemmed | Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC |
| title_short | Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC |
| title_sort | rapid and sensitive method for extraction of plicosepalus acacia with determination of its main polyphenolic compounds using validated hplc |
| url | http://dx.doi.org/10.1155/2020/9598606 |
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