Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts
Aspergillus fumigatus, is considered the most common human pathogen causing Aspergillosis, and is a tough opportunistic pathogen. This pathogen is very capable of forming biofilms via developing hyphal walls radially. The current therapies are known for their limitations where they couldn’t target...
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middle technical university
2021-12-01
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| Series: | Journal of Techniques |
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| Online Access: | https://journal.mtu.edu.iq/index.php/MTU/article/view/385 |
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| author | Omar Sadik Shalal |
| author_facet | Omar Sadik Shalal |
| author_sort | Omar Sadik Shalal |
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Aspergillus fumigatus, is considered the most common human pathogen causing Aspergillosis, and is a tough opportunistic pathogen. This pathogen is very capable of forming biofilms via developing hyphal walls radially. The current therapies are known for their limitations where they couldn’t target the proteins involved in undoing host response. Here we aimed to screen the possible antifungal role of Curcumin and its effect on the Transglutaminase 2 and Reactive Oxygen species response. We also screened the levels of cytokines with and without the treatment to confirm the activity. We could neutralize the elevated levels of TG2 by using the treatment (methanol extract). Even the cytokine levels could be normalized with the extract treatment which is usually highly elevated with the fungal infection. We found that TG2 and IL 1β and IL 18 got elevated significantly in control in addition, as well as our extract could confirm the possible role of ROS and its effect on TG2 which is said to play a major role in apoptosis.
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| format | Article |
| id | doaj-art-558e5ac8a8574a64bba2647f44e56267 |
| institution | Kabale University |
| issn | 1818-653X 2708-8383 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | middle technical university |
| record_format | Article |
| series | Journal of Techniques |
| spelling | doaj-art-558e5ac8a8574a64bba2647f44e562672025-01-19T11:07:21Zengmiddle technical universityJournal of Techniques1818-653X2708-83832021-12-013410.51173/jt.v3i4.385Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma ExtractsOmar Sadik Shalal0College of health and Medical Technology, Middle Technical University, Baghdad, Iraq Aspergillus fumigatus, is considered the most common human pathogen causing Aspergillosis, and is a tough opportunistic pathogen. This pathogen is very capable of forming biofilms via developing hyphal walls radially. The current therapies are known for their limitations where they couldn’t target the proteins involved in undoing host response. Here we aimed to screen the possible antifungal role of Curcumin and its effect on the Transglutaminase 2 and Reactive Oxygen species response. We also screened the levels of cytokines with and without the treatment to confirm the activity. We could neutralize the elevated levels of TG2 by using the treatment (methanol extract). Even the cytokine levels could be normalized with the extract treatment which is usually highly elevated with the fungal infection. We found that TG2 and IL 1β and IL 18 got elevated significantly in control in addition, as well as our extract could confirm the possible role of ROS and its effect on TG2 which is said to play a major role in apoptosis. https://journal.mtu.edu.iq/index.php/MTU/article/view/385Aspergillus fumigatusTransglutaminase 2ROSInterleukins |
| spellingShingle | Omar Sadik Shalal Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts Journal of Techniques Aspergillus fumigatus Transglutaminase 2 ROS Interleukins |
| title | Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts |
| title_full | Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts |
| title_fullStr | Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts |
| title_full_unstemmed | Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts |
| title_short | Expression Profiling of Transglutaminase 2 and Inflammatory Host Response in Aspergillus Fumigatus After Treatment with Curcuma Extracts |
| title_sort | expression profiling of transglutaminase 2 and inflammatory host response in aspergillus fumigatus after treatment with curcuma extracts |
| topic | Aspergillus fumigatus Transglutaminase 2 ROS Interleukins |
| url | https://journal.mtu.edu.iq/index.php/MTU/article/view/385 |
| work_keys_str_mv | AT omarsadikshalal expressionprofilingoftransglutaminase2andinflammatoryhostresponseinaspergillusfumigatusaftertreatmentwithcurcumaextracts |