Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions
Abstract Background Magnesium (Mg) is essential for plant growth and development and plays critical roles in physiological and biochemical processes. Mg deficiency adversely affects growth of plants by limiting shoot and root development, disturbing the structure and membranes of the grana, reducing...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-05985-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585911301308416 |
|---|---|
| author | Ammar Anwar Junaid Akhtar Saba Aleem Maida Aleem Muhammad Khuram Razzaq Saud Alamri Qasim Raza Iram Sharif Arooj Iftikhar Shehreen Naseer Zaheer Ahmed Iqrar Ahmed Rana Waheed Arshad Muhammad Imran Khan Javaid Akhter Bhat Muqadas Aleem Abdel-Rhman Z. Gaafar Mohamed S. Hodhod |
| author_facet | Ammar Anwar Junaid Akhtar Saba Aleem Maida Aleem Muhammad Khuram Razzaq Saud Alamri Qasim Raza Iram Sharif Arooj Iftikhar Shehreen Naseer Zaheer Ahmed Iqrar Ahmed Rana Waheed Arshad Muhammad Imran Khan Javaid Akhter Bhat Muqadas Aleem Abdel-Rhman Z. Gaafar Mohamed S. Hodhod |
| author_sort | Ammar Anwar |
| collection | DOAJ |
| description | Abstract Background Magnesium (Mg) is essential for plant growth and development and plays critical roles in physiological and biochemical processes. Mg deficiency adversely affects growth of plants by limiting shoot and root development, disturbing the structure and membranes of the grana, reducing photosynthesis efficiency, and lowering net CO2 assimilation. The MGT (Magnesium transporter) family is responsible for the absorption and transportation of magnesium in plants. Although the MGT family has been identified in different plant species, research regarding the soybean MGT genes is limited. Results In the current study, a total of 39 MGT genes distributed on 17 different chromosomes were identified in soybean. Phylogenetic analysis classified GmMGTs into three subgroups, NIPA, MRS2/MGT, and CorA, which showed little homology with MGTs of Arabidopsis thaliana and Oryza sativa members and clustered tightly with GmMGTs. Gene structure and conserved motif analysis also confirmed similar grouping in GmMGTs. The expansion of the GmMGT members in NIPA and MRS2/MGT was predicted, while CorA was identified as the most conserved group in G. max. Segmental duplication under purifying selection pressure was identified as the major driving force in the expansion of MGTs in soybean. GmMGTs showed diverse tissue-specific and stress-response expression patterns due to the presence of stress-related cis-regulatory elements in their promoter regions. Under Mg-deficiency and surplus stress conditions, a decrease in root length, shoot length, and root and shoot fresh as well dry weight in susceptible genotypes showed the variegated expression of MGTs in soybean genotypes. Furthermore, the upregulation of GmMGT2 and GmMGT29 in tolerant genotypes in response to Mg-deficiency as well as surplus stress conditions in leaves suggested the essential role of GmMGT genes in the absorption and transportation of Mg in soybean leaves. Conclusion This study presents a comprehensive analysis of the MGT gene family in soybean, providing insights into their evolutionary relationships, gene classification, protein structures, and expression patterns under both Mg deficiency and Mg surplus conditions. |
| format | Article |
| id | doaj-art-55712cf9b93c422b8193cfe708ff34c3 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-55712cf9b93c422b8193cfe708ff34c32025-01-26T12:23:11ZengBMCBMC Plant Biology1471-22292025-01-0125112010.1186/s12870-024-05985-7Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditionsAmmar Anwar0Junaid Akhtar1Saba Aleem2Maida Aleem3Muhammad Khuram Razzaq4Saud Alamri5Qasim Raza6Iram Sharif7Arooj Iftikhar8Shehreen Naseer9Zaheer Ahmed10Iqrar Ahmed Rana11Waheed Arshad12Muhammad Imran Khan13Javaid Akhter Bhat14Muqadas Aleem15Abdel-Rhman Z. Gaafar16Mohamed S. Hodhod17Department of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureBarani Agricultural Research Station, FatehjangAyub Agricultural Research InstituteDepartment of Botany, University of AgricultureFaculty of Agriculture and Veterinary Sciences, Superior UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Plant Breeding and Genetics, University of AgricultureCotton Research Station, Faisalabad, Ayub Agricultural Research InstituteDepartment of Environmental Sciences, University of Veterinary and Animal SciencesDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureCentre of Agricultural Biochemistry and Biotechnology (CABB), University of University of AgricultureBarani Agricultural Research Station, FatehjangAyub Agricultural Research InstituteBarani Agricultural Research Station, FatehjangAyub Agricultural Research InstituteZhejiang LabDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Botany and Microbiology, College of Science, King Saud UniversityFaculty of Biotechnology, October University for Modern Sciences & ArtsAbstract Background Magnesium (Mg) is essential for plant growth and development and plays critical roles in physiological and biochemical processes. Mg deficiency adversely affects growth of plants by limiting shoot and root development, disturbing the structure and membranes of the grana, reducing photosynthesis efficiency, and lowering net CO2 assimilation. The MGT (Magnesium transporter) family is responsible for the absorption and transportation of magnesium in plants. Although the MGT family has been identified in different plant species, research regarding the soybean MGT genes is limited. Results In the current study, a total of 39 MGT genes distributed on 17 different chromosomes were identified in soybean. Phylogenetic analysis classified GmMGTs into three subgroups, NIPA, MRS2/MGT, and CorA, which showed little homology with MGTs of Arabidopsis thaliana and Oryza sativa members and clustered tightly with GmMGTs. Gene structure and conserved motif analysis also confirmed similar grouping in GmMGTs. The expansion of the GmMGT members in NIPA and MRS2/MGT was predicted, while CorA was identified as the most conserved group in G. max. Segmental duplication under purifying selection pressure was identified as the major driving force in the expansion of MGTs in soybean. GmMGTs showed diverse tissue-specific and stress-response expression patterns due to the presence of stress-related cis-regulatory elements in their promoter regions. Under Mg-deficiency and surplus stress conditions, a decrease in root length, shoot length, and root and shoot fresh as well dry weight in susceptible genotypes showed the variegated expression of MGTs in soybean genotypes. Furthermore, the upregulation of GmMGT2 and GmMGT29 in tolerant genotypes in response to Mg-deficiency as well as surplus stress conditions in leaves suggested the essential role of GmMGT genes in the absorption and transportation of Mg in soybean leaves. Conclusion This study presents a comprehensive analysis of the MGT gene family in soybean, providing insights into their evolutionary relationships, gene classification, protein structures, and expression patterns under both Mg deficiency and Mg surplus conditions.https://doi.org/10.1186/s12870-024-05985-7Magnesium transporterGlycine maxMRS/MGTNIPACorAMg deficient |
| spellingShingle | Ammar Anwar Junaid Akhtar Saba Aleem Maida Aleem Muhammad Khuram Razzaq Saud Alamri Qasim Raza Iram Sharif Arooj Iftikhar Shehreen Naseer Zaheer Ahmed Iqrar Ahmed Rana Waheed Arshad Muhammad Imran Khan Javaid Akhter Bhat Muqadas Aleem Abdel-Rhman Z. Gaafar Mohamed S. Hodhod Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions BMC Plant Biology Magnesium transporter Glycine max MRS/MGT NIPA CorA Mg deficient |
| title | Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions |
| title_full | Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions |
| title_fullStr | Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions |
| title_full_unstemmed | Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions |
| title_short | Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions |
| title_sort | genome wide identification of mgt gene family in soybean glycine max and their expression analyses under magnesium stress conditions |
| topic | Magnesium transporter Glycine max MRS/MGT NIPA CorA Mg deficient |
| url | https://doi.org/10.1186/s12870-024-05985-7 |
| work_keys_str_mv | AT ammaranwar genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT junaidakhtar genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT sabaaleem genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT maidaaleem genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT muhammadkhuramrazzaq genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT saudalamri genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT qasimraza genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT iramsharif genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT aroojiftikhar genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT shehreennaseer genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT zaheerahmed genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT iqrarahmedrana genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT waheedarshad genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT muhammadimrankhan genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT javaidakhterbhat genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT muqadasaleem genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT abdelrhmanzgaafar genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions AT mohamedshodhod genomewideidentificationofmgtgenefamilyinsoybeanglycinemaxandtheirexpressionanalysesundermagnesiumstressconditions |