Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions
By using a high internal phase emulsion process, elastomeric poly(ε-caprolactone urethane) (PCLU) scaffolds were designed with pores size ranging from below 150 μm to 1800 μm and a porosity of 86% making them suitable for bone tissue engineering applications. Moreover, the pores appeared to be excel...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/283796 |
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| author | Sylvie Changotade Gabriela Radu Bostan Anne Consalus Florence Poirier Juliette Peltzer Jean-Jacques Lataillade Didier Lutomski Géraldine Rohman |
| author_facet | Sylvie Changotade Gabriela Radu Bostan Anne Consalus Florence Poirier Juliette Peltzer Jean-Jacques Lataillade Didier Lutomski Géraldine Rohman |
| author_sort | Sylvie Changotade |
| collection | DOAJ |
| description | By using a high internal phase emulsion process, elastomeric poly(ε-caprolactone urethane) (PCLU) scaffolds were designed with pores size ranging from below 150 μm to 1800 μm and a porosity of 86% making them suitable for bone tissue engineering applications. Moreover, the pores appeared to be excellently interconnected, promoting cellularization and future bone ingrowth. This study evaluated the in vitro cytotoxicity of the PCLU scaffolds towards human mesenchymal stem cells (hMSCs) through the evaluation of cell viability and metabolic activity during extract test and indirect contact test at the beginning of the scaffold lifetime. Both tests demonstrated that PCLU scaffolds did not induce any cytotoxic response. Finally, direct interaction of hMSCs and PCLU scaffolds showed that PCLU scaffolds were suitable for supporting the hMSCs adhesion and that the cells were well spread over the pore walls. We conclude that PCLU scaffolds may be a good candidate for bone tissue regeneration applications using hMSCs. |
| format | Article |
| id | doaj-art-5545f4c3125344cd8d7137b36845481b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5545f4c3125344cd8d7137b36845481b2025-02-03T01:20:30ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/283796283796Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase EmulsionsSylvie Changotade0Gabriela Radu Bostan1Anne Consalus2Florence Poirier3Juliette Peltzer4Jean-Jacques Lataillade5Didier Lutomski6Géraldine Rohman7Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceUniversité Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceUniversité Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceUniversité Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceInstitut de Recherche Biomédicale des Armées, Unité de Thérapie Cellulaire et Réparation Tissulaire, Site du Centre de Transfusion Sanguine des Armées “Jean Julliard” de Clamart, BP 73 91223 Brétigny-sur-Orge Cedex, FranceInstitut de Recherche Biomédicale des Armées, Unité de Thérapie Cellulaire et Réparation Tissulaire, Site du Centre de Transfusion Sanguine des Armées “Jean Julliard” de Clamart, BP 73 91223 Brétigny-sur-Orge Cedex, FranceUniversité Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceUniversité Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, FranceBy using a high internal phase emulsion process, elastomeric poly(ε-caprolactone urethane) (PCLU) scaffolds were designed with pores size ranging from below 150 μm to 1800 μm and a porosity of 86% making them suitable for bone tissue engineering applications. Moreover, the pores appeared to be excellently interconnected, promoting cellularization and future bone ingrowth. This study evaluated the in vitro cytotoxicity of the PCLU scaffolds towards human mesenchymal stem cells (hMSCs) through the evaluation of cell viability and metabolic activity during extract test and indirect contact test at the beginning of the scaffold lifetime. Both tests demonstrated that PCLU scaffolds did not induce any cytotoxic response. Finally, direct interaction of hMSCs and PCLU scaffolds showed that PCLU scaffolds were suitable for supporting the hMSCs adhesion and that the cells were well spread over the pore walls. We conclude that PCLU scaffolds may be a good candidate for bone tissue regeneration applications using hMSCs.http://dx.doi.org/10.1155/2015/283796 |
| spellingShingle | Sylvie Changotade Gabriela Radu Bostan Anne Consalus Florence Poirier Juliette Peltzer Jean-Jacques Lataillade Didier Lutomski Géraldine Rohman Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions Stem Cells International |
| title | Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions |
| title_full | Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions |
| title_fullStr | Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions |
| title_full_unstemmed | Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions |
| title_short | Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions |
| title_sort | preliminary in vitro assessment of stem cell compatibility with cross linked poly ε caprolactone urethane scaffolds designed through high internal phase emulsions |
| url | http://dx.doi.org/10.1155/2015/283796 |
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