Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field
This study was conducted to evaluate the optimal protein requirement of Procambarus clarkii cultured in paddy field, six isoenergetic and isolipid diets were formulated to contain graded levels of 28 %, 31 %, 34 %, 37 %, 40 % and 43 % crude protein (called P28–43 group). There were 3 replicates in e...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006987 |
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| author | Hui Luo Feng Qin Yu Li Chuanbo Xiao Wenbo Sun Fan Liu Guangjun Lv Hua Ye Yuanfa He Wenping He Feng Xu Yong Lin Dapeng Wang Honghao Jin |
| author_facet | Hui Luo Feng Qin Yu Li Chuanbo Xiao Wenbo Sun Fan Liu Guangjun Lv Hua Ye Yuanfa He Wenping He Feng Xu Yong Lin Dapeng Wang Honghao Jin |
| author_sort | Hui Luo |
| collection | DOAJ |
| description | This study was conducted to evaluate the optimal protein requirement of Procambarus clarkii cultured in paddy field, six isoenergetic and isolipid diets were formulated to contain graded levels of 28 %, 31 %, 34 %, 37 %, 40 % and 43 % crude protein (called P28–43 group). There were 3 replicates in each treatment and 20 crayfish in each replicate with an initial average body weight of (20.30 ± 0.24) g were fed for 56 days. Our results showed that final body weight was maximized in the P31 group, the weight gain rate and specific growth rate were maximized in the P34 group, all of which were significantly higher than those of P28 group (P < 0.05). Significant improvements in reproductive index, fecundity, egg diameter and egg production were found by increasing the dietary protein level from 28 % to 37 % (P < 0.05), while no significant increase was observed when the protein level was further increased to 43 % (P > 0.05). Total essential amino acids, total non-essential amino acids, and total amino acids in muscle were not significantly affected by dietary protein content, all of which were lowest in P28 group. Muscle protein content significantly increased as dietary protein level increasing and was highest in P37 group (P < 0.05). There was no significant difference in muscle moisture, crude lipid and crude ash content of among all groups (P > 0.05). P43 group showed the maximum levels of aspartate transaminase activity, with a significant difference (P < 0.05) compared to P31–37 groups. A positive correlation was observed between the dietary protein level and total bilirubin concentration. Dietary protein level had a significant impact on trypsin activity, with the P43 group showing significantly increased trypsin activity compared to the P28 group (P < 0.05). The activity of total superoxide dismutase, catalase, glutathione peroxidase, and lysozyme peaked in the P31 group and was significantly higher than in the P43 group (P < 0.05). The nitric oxide concentration was significantly higher in the P37 group compared to P28–34 and P43 groups (P < 0.05). The malondialdehyde content significantly increased as dietary protein level increasing, which was highest in the P43 group (P < 0.05). In conclusion, dietary protein level of 34.00 % provided superior growth performance for the P. clarkii broodstock, and the diets with 37.00 % protein were optimal for reproductive performance of P. clarkii broodstock in rice-crayfish culture. |
| format | Article |
| id | doaj-art-5531002857f648608c9f8f0386f52feb |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| spelling | doaj-art-5531002857f648608c9f8f0386f52feb2025-02-06T05:12:18ZengElsevierAquaculture Reports2352-51342025-03-0140102610Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice fieldHui Luo0Feng Qin1Yu Li2Chuanbo Xiao3Wenbo Sun4Fan Liu5Guangjun Lv6Hua Ye7Yuanfa He8Wenping He9Feng Xu10Yong Lin11Dapeng Wang12Honghao Jin13Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaChongqing General Station of Aquatic Technology Promotion, Chongqing 401121, ChinaGuangxi Academy of Fisheries Science, Nanning 530021, ChinaGuangxi Academy of Fisheries Science, Nanning 530021, China; Corresponding authors.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, China; Corresponding authors.This study was conducted to evaluate the optimal protein requirement of Procambarus clarkii cultured in paddy field, six isoenergetic and isolipid diets were formulated to contain graded levels of 28 %, 31 %, 34 %, 37 %, 40 % and 43 % crude protein (called P28–43 group). There were 3 replicates in each treatment and 20 crayfish in each replicate with an initial average body weight of (20.30 ± 0.24) g were fed for 56 days. Our results showed that final body weight was maximized in the P31 group, the weight gain rate and specific growth rate were maximized in the P34 group, all of which were significantly higher than those of P28 group (P < 0.05). Significant improvements in reproductive index, fecundity, egg diameter and egg production were found by increasing the dietary protein level from 28 % to 37 % (P < 0.05), while no significant increase was observed when the protein level was further increased to 43 % (P > 0.05). Total essential amino acids, total non-essential amino acids, and total amino acids in muscle were not significantly affected by dietary protein content, all of which were lowest in P28 group. Muscle protein content significantly increased as dietary protein level increasing and was highest in P37 group (P < 0.05). There was no significant difference in muscle moisture, crude lipid and crude ash content of among all groups (P > 0.05). P43 group showed the maximum levels of aspartate transaminase activity, with a significant difference (P < 0.05) compared to P31–37 groups. A positive correlation was observed between the dietary protein level and total bilirubin concentration. Dietary protein level had a significant impact on trypsin activity, with the P43 group showing significantly increased trypsin activity compared to the P28 group (P < 0.05). The activity of total superoxide dismutase, catalase, glutathione peroxidase, and lysozyme peaked in the P31 group and was significantly higher than in the P43 group (P < 0.05). The nitric oxide concentration was significantly higher in the P37 group compared to P28–34 and P43 groups (P < 0.05). The malondialdehyde content significantly increased as dietary protein level increasing, which was highest in the P43 group (P < 0.05). In conclusion, dietary protein level of 34.00 % provided superior growth performance for the P. clarkii broodstock, and the diets with 37.00 % protein were optimal for reproductive performance of P. clarkii broodstock in rice-crayfish culture.http://www.sciencedirect.com/science/article/pii/S2352513424006987Procambarus clarkiiDietary proteinGrowthReproductive performanceHealthRice-crayfish culture |
| spellingShingle | Hui Luo Feng Qin Yu Li Chuanbo Xiao Wenbo Sun Fan Liu Guangjun Lv Hua Ye Yuanfa He Wenping He Feng Xu Yong Lin Dapeng Wang Honghao Jin Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field Aquaculture Reports Procambarus clarkii Dietary protein Growth Reproductive performance Health Rice-crayfish culture |
| title | Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field |
| title_full | Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field |
| title_fullStr | Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field |
| title_full_unstemmed | Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field |
| title_short | Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field |
| title_sort | positive effects of optimal dietary protein content on the growth reproductive performance and health of procambarus clarkii broodstock farming in rice field |
| topic | Procambarus clarkii Dietary protein Growth Reproductive performance Health Rice-crayfish culture |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006987 |
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