Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method
Most of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological activity or yield antibodies which have lost much of their immunological specificity. The use of glutaraldehyde for coupling saves both the pharmacological and immunological activitie...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1992/398178 |
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| _version_ | 1832547430475759616 |
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| author | Michel Pagé Denis Thibeault Hélène Tremblay Christiane Noël Marie-Josée Perron |
| author_facet | Michel Pagé Denis Thibeault Hélène Tremblay Christiane Noël Marie-Josée Perron |
| author_sort | Michel Pagé |
| collection | DOAJ |
| description | Most
of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological
activity or yield antibodies which have lost much of their immunological specificity. The use of
glutaraldehyde for coupling saves both the pharmacological and immunological activities but it causes
some polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use.
A new coupling procedure is reported which uses an activated derivative of daunorubicin added to
monoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significant
polymerization of the antibody or Joss of pharmacological activity determined by testing normal and tumour
cell Lines in vitro. |
| format | Article |
| id | doaj-art-55227fa17d764bd995898d88e6a45955 |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-55227fa17d764bd995898d88e6a459552025-02-03T06:44:50ZengWileyCanadian Journal of Infectious Diseases1180-23321992-01-013Suppl B10110510.1155/1992/398178Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel MethodMichel PagéDenis ThibeaultHélène TremblayChristiane NoëlMarie-Josée PerronMost of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological activity or yield antibodies which have lost much of their immunological specificity. The use of glutaraldehyde for coupling saves both the pharmacological and immunological activities but it causes some polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use. A new coupling procedure is reported which uses an activated derivative of daunorubicin added to monoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significant polymerization of the antibody or Joss of pharmacological activity determined by testing normal and tumour cell Lines in vitro.http://dx.doi.org/10.1155/1992/398178 |
| spellingShingle | Michel Pagé Denis Thibeault Hélène Tremblay Christiane Noël Marie-Josée Perron Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method Canadian Journal of Infectious Diseases |
| title | Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method |
| title_full | Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method |
| title_fullStr | Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method |
| title_full_unstemmed | Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method |
| title_short | Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method |
| title_sort | conjugation of daunorubicin to monoclonal antihuman sarcoma antibody by a novel method |
| url | http://dx.doi.org/10.1155/1992/398178 |
| work_keys_str_mv | AT michelpage conjugationofdaunorubicintomonoclonalantihumansarcomaantibodybyanovelmethod AT denisthibeault conjugationofdaunorubicintomonoclonalantihumansarcomaantibodybyanovelmethod AT helenetremblay conjugationofdaunorubicintomonoclonalantihumansarcomaantibodybyanovelmethod AT christianenoel conjugationofdaunorubicintomonoclonalantihumansarcomaantibodybyanovelmethod AT mariejoseeperron conjugationofdaunorubicintomonoclonalantihumansarcomaantibodybyanovelmethod |