Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method
Most of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological activity or yield antibodies which have lost much of their immunological specificity. The use of glutaraldehyde for coupling saves both the pharmacological and immunological activitie...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1992/398178 |
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| Summary: | Most
of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological
activity or yield antibodies which have lost much of their immunological specificity. The use of
glutaraldehyde for coupling saves both the pharmacological and immunological activities but it causes
some polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use.
A new coupling procedure is reported which uses an activated derivative of daunorubicin added to
monoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significant
polymerization of the antibody or Joss of pharmacological activity determined by testing normal and tumour
cell Lines in vitro. |
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| ISSN: | 1180-2332 |