Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus
Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of s...
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Istanbul University Press
2022-01-01
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| Series: | Aquatic Sciences and Engineering |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/7A80032A133F4232AC441FE1E36924FA |
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| author | Neslihan Şener Zeliha Demirel Esra İmamoğlu Meltem Conk Dalay |
| author_facet | Neslihan Şener Zeliha Demirel Esra İmamoğlu Meltem Conk Dalay |
| author_sort | Neslihan Şener |
| collection | DOAJ |
| description | Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliella salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m2 s, and in the nutrient component of 8.82 mM NaNO3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus. |
| format | Article |
| id | doaj-art-551237bb3ebd4d6f871957ce8f3e4fc4 |
| institution | DOAJ |
| issn | 2602-473X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Aquatic Sciences and Engineering |
| spelling | doaj-art-551237bb3ebd4d6f871957ce8f3e4fc42025-08-20T03:09:30ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2022-01-01371293710.26650/ASE2020785091123456Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutusNeslihan Şener0https://orcid.org/0000-0002-4898-9562Zeliha Demirel1https://orcid.org/0000-0003-3675-7315Esra İmamoğlu2https://orcid.org/0000-0001-8759-7388Meltem Conk Dalay3https://orcid.org/0000-0002-1718-7292Ege Üniversitesi, Izmir, TurkiyeEge Üniversitesi, Izmir, TurkiyeEge Üniversitesi, Izmir, TurkiyeEge Üniversitesi, Izmir, TurkiyeCommercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliella salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m2 s, and in the nutrient component of 8.82 mM NaNO3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/7A80032A133F4232AC441FE1E36924FAchlorella vulgarisankistrodesmus convolutusdunaliella salinatetraselmis striatacarotenoidoptimizationphotobioreactor |
| spellingShingle | Neslihan Şener Zeliha Demirel Esra İmamoğlu Meltem Conk Dalay Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus Aquatic Sciences and Engineering chlorella vulgaris ankistrodesmus convolutus dunaliella salina tetraselmis striata carotenoid optimization photobioreactor |
| title | Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus |
| title_full | Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus |
| title_fullStr | Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus |
| title_full_unstemmed | Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus |
| title_short | Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus |
| title_sort | optimization of culture conditions for total carotenoid amount using response surface methodology in green microalgae ankistrodesmus convolutus |
| topic | chlorella vulgaris ankistrodesmus convolutus dunaliella salina tetraselmis striata carotenoid optimization photobioreactor |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/7A80032A133F4232AC441FE1E36924FA |
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