Urine metabolic profile in rats with arterial hypertension of different genesis
The diversity of pathogenetic mechanisms underlying arterial hypertension leads to the necessity to devise a personalized approach to the diagnosis and treatment of the disease. Metabolomics is one of the promising methods for personalized medicine, as it provides a comprehensive understanding of th...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-05-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/4143 |
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| author | A. A. Sorokoumova A. A. Seryapina Yu. K. Polityko L. V. Yanshole Yu. P. Tsentalovich М. А. Gilinsky А. L. Markel |
| author_facet | A. A. Sorokoumova A. A. Seryapina Yu. K. Polityko L. V. Yanshole Yu. P. Tsentalovich М. А. Gilinsky А. L. Markel |
| author_sort | A. A. Sorokoumova |
| collection | DOAJ |
| description | The diversity of pathogenetic mechanisms underlying arterial hypertension leads to the necessity to devise a personalized approach to the diagnosis and treatment of the disease. Metabolomics is one of the promising methods for personalized medicine, as it provides a comprehensive understanding of the physiological processes occurring in the body. The metabolome is a set of low-molecular substances available for detection in a sample and representing intermediate and final products of cell metabolism. Changes in the content and ratio of metabolites in the sample mark the corresponding pathogenetic mechanisms by highlighting them, which is especially important for such a multifactorial disease as arterial hypertension. To identify metabolomic markers for hypertensive conditions of different origins, three forms of arterial hypertension (AH) were studied: rats with hereditary AH (ISIAH rat strain); rats with AH induced by L-NAME administration (a model of endothelial dysfunction with impaired NO production); rats with AH caused by the administration of deoxycorticosterone in combination with salt loading (hormone-dependent form – DOCAsalt AH). WAG rats were used as normotensive controls. 24-hour urine samples were collected from all animals and analyzed by quantitative NMR spectroscopy for metabolic profiling. Then, potential metabolomic markers for the studied forms of hypertensive conditions were identified using multivariate statistics. Analysis of the data obtained showed that hereditary stress-induced arterial hypertension in ISIAH rats was characterized by a decrease in the following urine metabolites: nicotinamide and 1-methylnicotinamide (markers of inflammatory processes), N- ace tyl glutamate (nitric oxide cycle), isobutyrate and methyl acetoacetate (gut microbiota). Pharmacologically induced forms of hypertension (the L-NAME and DOCA+NaCl groups) do not share metabolomic markers with hereditary AH. They are differentiated by N,N-dimethylglycine (both groups), choline (the L-NAME group) and 1-methylnicotinamide (the group of rats with DOCA-salt hypertension). |
| format | Article |
| id | doaj-art-551019d1cf3842e6819e7af201021dd5 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-551019d1cf3842e6819e7af201021dd52025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-05-0128329930710.18699/vjgb-24-341464Urine metabolic profile in rats with arterial hypertension of different genesisA. A. Sorokoumova0A. A. Seryapina1Yu. K. Polityko2L. V. Yanshole3Yu. P. Tsentalovich4М. А. Gilinsky5А. L. Markel6Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Scientific Research Institute of Neurosciences and MedicineInternational Tomography Center of the Siberian Branch of the Russian Academy of SciencesInternational Tomography Center of the Siberian Branch of the Russian Academy of SciencesScientific Research Institute of Neurosciences and MedicineInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityThe diversity of pathogenetic mechanisms underlying arterial hypertension leads to the necessity to devise a personalized approach to the diagnosis and treatment of the disease. Metabolomics is one of the promising methods for personalized medicine, as it provides a comprehensive understanding of the physiological processes occurring in the body. The metabolome is a set of low-molecular substances available for detection in a sample and representing intermediate and final products of cell metabolism. Changes in the content and ratio of metabolites in the sample mark the corresponding pathogenetic mechanisms by highlighting them, which is especially important for such a multifactorial disease as arterial hypertension. To identify metabolomic markers for hypertensive conditions of different origins, three forms of arterial hypertension (AH) were studied: rats with hereditary AH (ISIAH rat strain); rats with AH induced by L-NAME administration (a model of endothelial dysfunction with impaired NO production); rats with AH caused by the administration of deoxycorticosterone in combination with salt loading (hormone-dependent form – DOCAsalt AH). WAG rats were used as normotensive controls. 24-hour urine samples were collected from all animals and analyzed by quantitative NMR spectroscopy for metabolic profiling. Then, potential metabolomic markers for the studied forms of hypertensive conditions were identified using multivariate statistics. Analysis of the data obtained showed that hereditary stress-induced arterial hypertension in ISIAH rats was characterized by a decrease in the following urine metabolites: nicotinamide and 1-methylnicotinamide (markers of inflammatory processes), N- ace tyl glutamate (nitric oxide cycle), isobutyrate and methyl acetoacetate (gut microbiota). Pharmacologically induced forms of hypertension (the L-NAME and DOCA+NaCl groups) do not share metabolomic markers with hereditary AH. They are differentiated by N,N-dimethylglycine (both groups), choline (the L-NAME group) and 1-methylnicotinamide (the group of rats with DOCA-salt hypertension).https://vavilov.elpub.ru/jour/article/view/4143arterial hypertensionisiah ratsl-namedoca-salt hypertensionurine metabolomic markers |
| spellingShingle | A. A. Sorokoumova A. A. Seryapina Yu. K. Polityko L. V. Yanshole Yu. P. Tsentalovich М. А. Gilinsky А. L. Markel Urine metabolic profile in rats with arterial hypertension of different genesis Вавиловский журнал генетики и селекции arterial hypertension isiah rats l-name doca-salt hypertension urine metabolomic markers |
| title | Urine metabolic profile in rats with arterial hypertension of different genesis |
| title_full | Urine metabolic profile in rats with arterial hypertension of different genesis |
| title_fullStr | Urine metabolic profile in rats with arterial hypertension of different genesis |
| title_full_unstemmed | Urine metabolic profile in rats with arterial hypertension of different genesis |
| title_short | Urine metabolic profile in rats with arterial hypertension of different genesis |
| title_sort | urine metabolic profile in rats with arterial hypertension of different genesis |
| topic | arterial hypertension isiah rats l-name doca-salt hypertension urine metabolomic markers |
| url | https://vavilov.elpub.ru/jour/article/view/4143 |
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