Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis
IntroductionColorectal cancer (CRC) is a prevalent malignancy worldwide with high mortality rate. Metastasis, the primary cause of cancer-related deaths, is attributed to various factors including tumor hypoxia. Due to the urgent demand for potent anti-metastatic agents, we aimed to determine the ef...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1520881/full |
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| author | Fatemeh B. Rassouli Maryam M. Matin Maryam M. Matin Farzin Hadizadeh Farzin Hadizadeh Masoud Nejabat Hossein Allahverdizadeh Hamidreza Jamali Shahin Gharedaghi Halimeh Hassanzadeh |
| author_facet | Fatemeh B. Rassouli Maryam M. Matin Maryam M. Matin Farzin Hadizadeh Farzin Hadizadeh Masoud Nejabat Hossein Allahverdizadeh Hamidreza Jamali Shahin Gharedaghi Halimeh Hassanzadeh |
| author_sort | Fatemeh B. Rassouli |
| collection | DOAJ |
| description | IntroductionColorectal cancer (CRC) is a prevalent malignancy worldwide with high mortality rate. Metastasis, the primary cause of cancer-related deaths, is attributed to various factors including tumor hypoxia. Due to the urgent demand for potent anti-metastatic agents, we aimed to determine the effects of sunitinib and novel analogs on the metastatic behavior of human CRC cells in hypoxic condition for the first time.MethodsFor in silico analyses, pathogenic targets of metastatic CRC were identified, PPI network was constructed and KEGG pathway enrichment analysis was conducted. The expression of HIF1A was evaluated in seven CRC cell lines, and computational modeling was carried out to define the interaction of sunitinib with HIF-1α. For in vitro studies, analogs of sunitinib were synthesized, and cells were assessed for viability, migration, invasion, MMPs activity and gene expression in hypoxic condition.Results and DiscussionComputational analyses highlighted the importance of HIF-1α as a crucial mediator of metastasis in CRC. Molecular docking and dynamics simulations demonstrated favorable and stable interaction of sunitinib and three novel analogs with HIF-1α PAS-B domain. Volcano plots indicated upregulation of HIF1A in LoVo cells compared to six other CRC cell lines. Findings of in vitro studies revealed considerable inhibitory effects of sunitinib and analogs on LoVo cell migration and invasion in hypoxic condition. Gelatin zymography and qPCR analysis indicated decreased activity of MMP-2 and MMP-9, along with downregulation of EMT transcription factors in hypoxic condition. Current study reports promising anti-metastatic effects of sunitinib and novel analogs on CRC cells, providing foundation for further investigation to combat cancer metastasis. |
| format | Article |
| id | doaj-art-550549b9bedf442bbc3a413ec44abe43 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-550549b9bedf442bbc3a413ec44abe432025-02-04T06:32:04ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011610.3389/fphar.2025.15208811520881Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasisFatemeh B. Rassouli0Maryam M. Matin1Maryam M. Matin2Farzin Hadizadeh3Farzin Hadizadeh4Masoud Nejabat5Hossein Allahverdizadeh6Hamidreza Jamali7Shahin Gharedaghi8Halimeh Hassanzadeh9Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranNovel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranNovel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranStem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research (ACECR)-Khorasan Razavi, Mashhad, IranIntroductionColorectal cancer (CRC) is a prevalent malignancy worldwide with high mortality rate. Metastasis, the primary cause of cancer-related deaths, is attributed to various factors including tumor hypoxia. Due to the urgent demand for potent anti-metastatic agents, we aimed to determine the effects of sunitinib and novel analogs on the metastatic behavior of human CRC cells in hypoxic condition for the first time.MethodsFor in silico analyses, pathogenic targets of metastatic CRC were identified, PPI network was constructed and KEGG pathway enrichment analysis was conducted. The expression of HIF1A was evaluated in seven CRC cell lines, and computational modeling was carried out to define the interaction of sunitinib with HIF-1α. For in vitro studies, analogs of sunitinib were synthesized, and cells were assessed for viability, migration, invasion, MMPs activity and gene expression in hypoxic condition.Results and DiscussionComputational analyses highlighted the importance of HIF-1α as a crucial mediator of metastasis in CRC. Molecular docking and dynamics simulations demonstrated favorable and stable interaction of sunitinib and three novel analogs with HIF-1α PAS-B domain. Volcano plots indicated upregulation of HIF1A in LoVo cells compared to six other CRC cell lines. Findings of in vitro studies revealed considerable inhibitory effects of sunitinib and analogs on LoVo cell migration and invasion in hypoxic condition. Gelatin zymography and qPCR analysis indicated decreased activity of MMP-2 and MMP-9, along with downregulation of EMT transcription factors in hypoxic condition. Current study reports promising anti-metastatic effects of sunitinib and novel analogs on CRC cells, providing foundation for further investigation to combat cancer metastasis.https://www.frontiersin.org/articles/10.3389/fphar.2025.1520881/fullcolon cancermetastasissunitinibnovel analogshypoxia |
| spellingShingle | Fatemeh B. Rassouli Maryam M. Matin Maryam M. Matin Farzin Hadizadeh Farzin Hadizadeh Masoud Nejabat Hossein Allahverdizadeh Hamidreza Jamali Shahin Gharedaghi Halimeh Hassanzadeh Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis Frontiers in Pharmacology colon cancer metastasis sunitinib novel analogs hypoxia |
| title | Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis |
| title_full | Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis |
| title_fullStr | Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis |
| title_full_unstemmed | Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis |
| title_short | Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis |
| title_sort | exploring the anti metastatic potential of sunitinib and novel analogs in colorectal cancer insights into hif 1α mediated metastasis |
| topic | colon cancer metastasis sunitinib novel analogs hypoxia |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1520881/full |
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