Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population

Abstract Mitochondrial genome is an essential resource to investigate the dispersion and diversification of human populations. However, the lack of genomic data from non-European ancestry hinders demographic, anthropologic and genetic studies. Indigenous people from the Brazilian Amazon are especial...

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Main Authors: Felipe Gouvea de Souza, Gustavo Barra Matos, Camille Sena Santos, Tatiane Piedade Souza, Angélica Rita Gobbo, Patrícia Fagundes da Costa, Claudio Guedes Salgado, Gracivane Lopes Eufraseo, André Vitor de Souza Fernandes, Bruno Lopes Santos-Lobato, Sidney Santos, Giovanna C. Cavalcante, André M. Ribeiro dos Santos, Gilderlanio S. de Araújo, Ândrea Ribeiro- dos-Santos
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08126-4
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author Felipe Gouvea de Souza
Gustavo Barra Matos
Camille Sena Santos
Tatiane Piedade Souza
Angélica Rita Gobbo
Patrícia Fagundes da Costa
Claudio Guedes Salgado
Gracivane Lopes Eufraseo
André Vitor de Souza Fernandes
Bruno Lopes Santos-Lobato
Sidney Santos
Giovanna C. Cavalcante
André M. Ribeiro dos Santos
Gilderlanio S. de Araújo
Ândrea Ribeiro- dos-Santos
author_facet Felipe Gouvea de Souza
Gustavo Barra Matos
Camille Sena Santos
Tatiane Piedade Souza
Angélica Rita Gobbo
Patrícia Fagundes da Costa
Claudio Guedes Salgado
Gracivane Lopes Eufraseo
André Vitor de Souza Fernandes
Bruno Lopes Santos-Lobato
Sidney Santos
Giovanna C. Cavalcante
André M. Ribeiro dos Santos
Gilderlanio S. de Araújo
Ândrea Ribeiro- dos-Santos
author_sort Felipe Gouvea de Souza
collection DOAJ
description Abstract Mitochondrial genome is an essential resource to investigate the dispersion and diversification of human populations. However, the lack of genomic data from non-European ancestry hinders demographic, anthropologic and genetic studies. Indigenous people from the Brazilian Amazon are especially underrepresented among large genetic datasets, despite their significative contribution to the Brazilian genetic pool. We investigated 157 mitochondrial genomes from the Brazilian Amazon using bioinformatics tools for ancestry inference and phylogenetics. Results were compared with 2578 global mitogenomes to assess haplogroup frequencies and genetic distances. Thirteen macrohaplogroups were identified from investigated samples, including Indigenous, European and African ancestries. Despite being prominent in our sample, Indigenous haplogroup assignment confidence by prediction tools was lower and many samples lacked key-defining variants, suggesting misassignment due to low representation in reference datasets. We also identified potential new groups sharing specific variants. Thus, underrepresentation of Indigenous haplogroups contributes to phylogenetic inconsistencies and needs future investigations.
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spelling doaj-art-54ff0583bb4e4e69b04294a9e80141ca2025-08-20T02:00:09ZengNature PortfolioCommunications Biology2399-36422025-05-01811910.1038/s42003-025-08126-4Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon populationFelipe Gouvea de Souza0Gustavo Barra Matos1Camille Sena Santos2Tatiane Piedade Souza3Angélica Rita Gobbo4Patrícia Fagundes da Costa5Claudio Guedes Salgado6Gracivane Lopes Eufraseo7André Vitor de Souza Fernandes8Bruno Lopes Santos-Lobato9Sidney Santos10Giovanna C. Cavalcante11André M. Ribeiro dos Santos12Gilderlanio S. de Araújo13Ândrea Ribeiro- dos-Santos14Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Dermato-Immunology (LDI), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Dermato-Immunology (LDI), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Dermato-Immunology (LDI), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Experimental Neurology (LaNEx), Federal University of Pará (UFPA)Laboratory of Experimental Neurology (LaNEx), Federal University of Pará (UFPA)Laboratory of Experimental Neurology (LaNEx), Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Laboratory of Human and Medical Genetics (LGHM), Institute of Biological Sciences, Federal University of Pará (UFPA)Abstract Mitochondrial genome is an essential resource to investigate the dispersion and diversification of human populations. However, the lack of genomic data from non-European ancestry hinders demographic, anthropologic and genetic studies. Indigenous people from the Brazilian Amazon are especially underrepresented among large genetic datasets, despite their significative contribution to the Brazilian genetic pool. We investigated 157 mitochondrial genomes from the Brazilian Amazon using bioinformatics tools for ancestry inference and phylogenetics. Results were compared with 2578 global mitogenomes to assess haplogroup frequencies and genetic distances. Thirteen macrohaplogroups were identified from investigated samples, including Indigenous, European and African ancestries. Despite being prominent in our sample, Indigenous haplogroup assignment confidence by prediction tools was lower and many samples lacked key-defining variants, suggesting misassignment due to low representation in reference datasets. We also identified potential new groups sharing specific variants. Thus, underrepresentation of Indigenous haplogroups contributes to phylogenetic inconsistencies and needs future investigations.https://doi.org/10.1038/s42003-025-08126-4
spellingShingle Felipe Gouvea de Souza
Gustavo Barra Matos
Camille Sena Santos
Tatiane Piedade Souza
Angélica Rita Gobbo
Patrícia Fagundes da Costa
Claudio Guedes Salgado
Gracivane Lopes Eufraseo
André Vitor de Souza Fernandes
Bruno Lopes Santos-Lobato
Sidney Santos
Giovanna C. Cavalcante
André M. Ribeiro dos Santos
Gilderlanio S. de Araújo
Ândrea Ribeiro- dos-Santos
Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
Communications Biology
title Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
title_full Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
title_fullStr Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
title_full_unstemmed Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
title_short Mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in Brazilian Amazon population
title_sort mitochondrial ancestry from complete mitogenomes highlights a lack of characterization of indigenous haplogroups in brazilian amazon population
url https://doi.org/10.1038/s42003-025-08126-4
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