Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS
Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different s...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/698630 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546132786413568 |
|---|---|
| author | Zhi-hong Huang Zhi-li Wang Bao-lin Shi Dong Wei Jian-xin Chen Su-li Wang Bao-jia Gao |
| author_facet | Zhi-hong Huang Zhi-li Wang Bao-lin Shi Dong Wei Jian-xin Chen Su-li Wang Bao-jia Gao |
| author_sort | Zhi-hong Huang |
| collection | DOAJ |
| description | Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite. |
| format | Article |
| id | doaj-art-54b2e4685e5e4883a383962be0e2a28f |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-54b2e4685e5e4883a383962be0e2a28f2025-02-03T07:23:51ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/698630698630Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MSZhi-hong Huang0Zhi-li Wang1Bao-lin Shi2Dong Wei3Jian-xin Chen4Su-li Wang5Bao-jia Gao6Agricultural University of Hebei, Baoding 071001, ChinaHebei North University, Zhangjiakou 075000, ChinaHebei North University, Zhangjiakou 075000, ChinaHebei North University, Zhangjiakou 075000, ChinaHebei North University, Zhangjiakou 075000, ChinaHebei North University, Zhangjiakou 075000, ChinaAgricultural University of Hebei, Baoding 071001, ChinaSalicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.http://dx.doi.org/10.1155/2015/698630 |
| spellingShingle | Zhi-hong Huang Zhi-li Wang Bao-lin Shi Dong Wei Jian-xin Chen Su-li Wang Bao-jia Gao Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS International Journal of Analytical Chemistry |
| title | Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS |
| title_full | Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS |
| title_fullStr | Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS |
| title_full_unstemmed | Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS |
| title_short | Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS |
| title_sort | simultaneous determination of salicylic acid jasmonic acid methyl salicylate and methyl jasmonate from ulmus pumila leaves by gc ms |
| url | http://dx.doi.org/10.1155/2015/698630 |
| work_keys_str_mv | AT zhihonghuang simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT zhiliwang simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT baolinshi simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT dongwei simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT jianxinchen simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT suliwang simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms AT baojiagao simultaneousdeterminationofsalicylicacidjasmonicacidmethylsalicylateandmethyljasmonatefromulmuspumilaleavesbygcms |