The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew
Blumeria graminis f. sp. Tritici causes wheat powdery mildew (WPM) and severe wheat damage worldwide. To prevent WPM and emerging chemical fungicide resistance, researchers investigated the control effectiveness and induced disease resistance mechanisms of the biocontrol agent Bacillus tequilensis....
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000088 |
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| author | Qiuyan Bi Fen Lu Jie Wu Xiangyu Liu Xiuying Han Jianjiang Zhao |
| author_facet | Qiuyan Bi Fen Lu Jie Wu Xiangyu Liu Xiuying Han Jianjiang Zhao |
| author_sort | Qiuyan Bi |
| collection | DOAJ |
| description | Blumeria graminis f. sp. Tritici causes wheat powdery mildew (WPM) and severe wheat damage worldwide. To prevent WPM and emerging chemical fungicide resistance, researchers investigated the control effectiveness and induced disease resistance mechanisms of the biocontrol agent Bacillus tequilensis. Its inhibitory effect was determined via an in vitro method combined with microscopic observation, and the control effect was clarified via pot and field verification. Using transcriptome technology, we determined the differences in wheat gene expression in after treatment with B. tequilensis and verified the main molecular mechanism of B. tequilensis-induced resistance. The effects on wheat defence enzymes were clarified via physiological and biochemical techniques. The results revealed that B. tequilensis controlled WPM at an effective concentration of 1 × 107 cfu/mL or higher. The inhibitory effect in vitro was 90.91–100.00 %. The potted control effect was 92.98–100.00 %. The field control effect was 83.21–100.00 %. Transcriptome sequencing analysis revealed 1,472 significantly upregulated genes and 1,995 downregulated genes. The differentially expressed genes were enriched in several pathways related to amino acid biosynthesis and metabolism. The expression levels of actin-7-like, lipoxygenase, linoleic acid 9S-lipoxygenase 6-like, pathogenesis-related protein PR-1-like, superoxide dismutase, phenylalanine ammonia lyase and polyphenol oxidase were significantly increased. The relative enzymic activities induced by B. tequilensis presented maximum differences between 2.63-fold and 3.43-fold. Our results suggest that B. tequilensis combats WPM by inducing systemic acquired resistance in wheat, helps elucidate biological agent targeted control mechanisms and provides a foundation for field application. |
| format | Article |
| id | doaj-art-54a588b93cfc4d038f714f81376f1f55 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-54a588b93cfc4d038f714f81376f1f552025-02-05T04:31:10ZengElsevierBiological Control1049-96442025-02-01201105698The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildewQiuyan Bi0Fen Lu1Jie Wu2Xiangyu Liu3Xiuying Han4Jianjiang Zhao5Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei Province; Corresponding authors.Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei ProvincePlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei ProvincePlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei ProvincePlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei ProvincePlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, P. R. China/ IPM Innovation Center of Hebei Province/ International Science and Technology Joint Research Center on IPM of Hebei Province; Corresponding authors.Blumeria graminis f. sp. Tritici causes wheat powdery mildew (WPM) and severe wheat damage worldwide. To prevent WPM and emerging chemical fungicide resistance, researchers investigated the control effectiveness and induced disease resistance mechanisms of the biocontrol agent Bacillus tequilensis. Its inhibitory effect was determined via an in vitro method combined with microscopic observation, and the control effect was clarified via pot and field verification. Using transcriptome technology, we determined the differences in wheat gene expression in after treatment with B. tequilensis and verified the main molecular mechanism of B. tequilensis-induced resistance. The effects on wheat defence enzymes were clarified via physiological and biochemical techniques. The results revealed that B. tequilensis controlled WPM at an effective concentration of 1 × 107 cfu/mL or higher. The inhibitory effect in vitro was 90.91–100.00 %. The potted control effect was 92.98–100.00 %. The field control effect was 83.21–100.00 %. Transcriptome sequencing analysis revealed 1,472 significantly upregulated genes and 1,995 downregulated genes. The differentially expressed genes were enriched in several pathways related to amino acid biosynthesis and metabolism. The expression levels of actin-7-like, lipoxygenase, linoleic acid 9S-lipoxygenase 6-like, pathogenesis-related protein PR-1-like, superoxide dismutase, phenylalanine ammonia lyase and polyphenol oxidase were significantly increased. The relative enzymic activities induced by B. tequilensis presented maximum differences between 2.63-fold and 3.43-fold. Our results suggest that B. tequilensis combats WPM by inducing systemic acquired resistance in wheat, helps elucidate biological agent targeted control mechanisms and provides a foundation for field application.http://www.sciencedirect.com/science/article/pii/S1049964425000088Bacillus tequilensisBlumeria graminisSystemic acquired resistance |
| spellingShingle | Qiuyan Bi Fen Lu Jie Wu Xiangyu Liu Xiuying Han Jianjiang Zhao The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew Biological Control Bacillus tequilensis Blumeria graminis Systemic acquired resistance |
| title | The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew |
| title_full | The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew |
| title_fullStr | The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew |
| title_full_unstemmed | The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew |
| title_short | The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew |
| title_sort | control effect and induced disease resistance mechanism of bacillus tequilensis on wheat powdery mildew |
| topic | Bacillus tequilensis Blumeria graminis Systemic acquired resistance |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000088 |
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