Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system

Anaerobic digestion (AD) is commonly used to dispose of laying hen manure. However, veterinary antibiotic residues present in chicken manure may affect the AD process. Here, the effects of three types of veterinary antibiotics commonly used in laying hen breeding on AD were explored. Manures contain...

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Main Authors: Yiting Wang, Qing Liu, Hongli Ran, Pingcai Peng, Yan Wang, Guoliang Peng, Yinbao Wu, Xin Wen
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124011179
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author Yiting Wang
Qing Liu
Hongli Ran
Pingcai Peng
Yan Wang
Guoliang Peng
Yinbao Wu
Xin Wen
author_facet Yiting Wang
Qing Liu
Hongli Ran
Pingcai Peng
Yan Wang
Guoliang Peng
Yinbao Wu
Xin Wen
author_sort Yiting Wang
collection DOAJ
description Anaerobic digestion (AD) is commonly used to dispose of laying hen manure. However, veterinary antibiotic residues present in chicken manure may affect the AD process. Here, the effects of three types of veterinary antibiotics commonly used in laying hen breeding on AD were explored. Manures containing antibiotics at two different concentrations were continuously added during AD for 5 days: amoxicillin (HAMX: 145.06 mg/kg, LAMX: 57.88 mg/kg), doxycycline (HDOC: 183.61 mg/kg, LDOC: 98.00 mg/kg), and ciprofloxacin (HCIP: 96.34 mg/kg, LCIP: 40.43 mg/kg). Compared with a control with no veterinary antibiotics, the amoxicillin and doxycycline groups presented no significant effects on biogas production, methane production, VFA concentration, acetic acid concentration or the pH of the AD system (P > 0.05). However, compared with the control, the ciprofloxacin groups presented significantly inhibited biogas and methane production during AD (P < 0.05), and the HCIP and LCIP groups presented significantly decreased biogas (47.82% and 45.37%, respectively) and methane (58.24% and 52.55%, respectively) production (P < 0.05). Moreover, the VFA and acetic acid concentrations of the ciprofloxacin groups were significantly higher than those of control during the entire AD period (P < 0.05), and the pH value at the withdrawal stage was significantly lower than that of the control group (P < 0.01), with no significant difference between the HCIP and LCIP groups (P > 0.05). Our results suggest that ciprofloxacin causes VFA and acetic acid accumulation in AD systems, thereby reducing the pH of the systems and inhibiting methanogen growth, ultimately reducing methane production in the AD systems. These findings contribute to a deeper understanding of the impact of ciprofloxacin on methane production in AD systems and offer some considerations for the application of AD systems.
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institution Kabale University
issn 0032-5791
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Poultry Science
spelling doaj-art-54955de094cd4761a454ba7fc48664612025-01-22T05:40:24ZengElsevierPoultry Science0032-57912025-01-011041104539Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion systemYiting Wang0Qing Liu1Hongli Ran2Pingcai Peng3Yan Wang4Guoliang Peng5Yinbao Wu6Xin Wen7College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaGuangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, and the Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, China; Corresponding author.Anaerobic digestion (AD) is commonly used to dispose of laying hen manure. However, veterinary antibiotic residues present in chicken manure may affect the AD process. Here, the effects of three types of veterinary antibiotics commonly used in laying hen breeding on AD were explored. Manures containing antibiotics at two different concentrations were continuously added during AD for 5 days: amoxicillin (HAMX: 145.06 mg/kg, LAMX: 57.88 mg/kg), doxycycline (HDOC: 183.61 mg/kg, LDOC: 98.00 mg/kg), and ciprofloxacin (HCIP: 96.34 mg/kg, LCIP: 40.43 mg/kg). Compared with a control with no veterinary antibiotics, the amoxicillin and doxycycline groups presented no significant effects on biogas production, methane production, VFA concentration, acetic acid concentration or the pH of the AD system (P > 0.05). However, compared with the control, the ciprofloxacin groups presented significantly inhibited biogas and methane production during AD (P < 0.05), and the HCIP and LCIP groups presented significantly decreased biogas (47.82% and 45.37%, respectively) and methane (58.24% and 52.55%, respectively) production (P < 0.05). Moreover, the VFA and acetic acid concentrations of the ciprofloxacin groups were significantly higher than those of control during the entire AD period (P < 0.05), and the pH value at the withdrawal stage was significantly lower than that of the control group (P < 0.01), with no significant difference between the HCIP and LCIP groups (P > 0.05). Our results suggest that ciprofloxacin causes VFA and acetic acid accumulation in AD systems, thereby reducing the pH of the systems and inhibiting methanogen growth, ultimately reducing methane production in the AD systems. These findings contribute to a deeper understanding of the impact of ciprofloxacin on methane production in AD systems and offer some considerations for the application of AD systems.http://www.sciencedirect.com/science/article/pii/S0032579124011179Laying hen manureCiprofloxacinAnaerobic digestionMethaneVeterinary antibiotic
spellingShingle Yiting Wang
Qing Liu
Hongli Ran
Pingcai Peng
Yan Wang
Guoliang Peng
Yinbao Wu
Xin Wen
Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
Poultry Science
Laying hen manure
Ciprofloxacin
Anaerobic digestion
Methane
Veterinary antibiotic
title Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
title_full Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
title_fullStr Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
title_full_unstemmed Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
title_short Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
title_sort residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system
topic Laying hen manure
Ciprofloxacin
Anaerobic digestion
Methane
Veterinary antibiotic
url http://www.sciencedirect.com/science/article/pii/S0032579124011179
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