Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates
Abstract: Little is known about the muscle developmental patterns during embryonic to neonatal development in ducks. We investigated the developmental patterns in the lateral gastrocnemius muscles of Gaoyou and Jinding ducks differing in their muscle growth rates during the final stages of egg incub...
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KeAi Communications Co., Ltd.
2016-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914609497 |
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| author | Hui-fang LI Jing-ting SHU Yan-ju SHAN Wen-feng CHEN Chi SONG Wen-juan XU |
| author_facet | Hui-fang LI Jing-ting SHU Yan-ju SHAN Wen-feng CHEN Chi SONG Wen-juan XU |
| author_sort | Hui-fang LI |
| collection | DOAJ |
| description | Abstract: Little is known about the muscle developmental patterns during embryonic to neonatal development in ducks. We investigated the developmental patterns in the lateral gastrocnemius muscles of Gaoyou and Jinding ducks differing in their muscle growth rates during the final stages of egg incubation and the first week after hatching. Expression of the MyoD gene was quantified by quantitative real-time PCR (qRT-PCR). The average cross-sectional area and diameter of the fibers increased from embryonic day 21 (E21), peaking at E27, and then declining slightly 7 d after hatching. The density of the fibers decreased initially but increased after hatching in both breeds and sexes. The within-breed variation in muscle fiber-type composition was greater than the average variation between the breeds. Overall, the percentage of type I fibers increased and that of type IIb fibers decreased consistently. However, the percentage of type IIa fibers was almost constant as development proceeded in both duck breeds. The profiles of MyoD mRNA expression were similar in both breeds, and a significantly positive relationship was observed between the expression of MyoD and the percentage of type IIb fibers. This study firstly revealed the characteristics of duck muscle development and differences between the two breeds differing in growth rates. Moreover, type IIb fibers might convert to type I fibers in the lateral gastrocnemius, while MyoD may potentially function in controlling the muscle fiber phenotype during the secondary myogenesis of muscle development. |
| format | Article |
| id | doaj-art-5482cd5811a64308a919c9bd1403e076 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-5482cd5811a64308a919c9bd1403e0762025-08-20T03:56:12ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-02-0115240341310.1016/S2095-3119(14)60949-7Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth ratesHui-fang LI0Jing-ting SHU1Yan-ju SHAN2Wen-feng CHEN3Chi SONG4Wen-juan XU5Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou 225125, P.R.China; Correspondence LI Hui-fang, Tel/Fax: +86-514-85599266Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou 225125, P.R.China; SHU Jing-tingKey Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou 225125, P.R.ChinaJiangsu Tengdayuan Co. Ltd., Jiangyan 225538, P.R.ChinaKey Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou 225125, P.R.ChinaKey Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou 225125, P.R.ChinaAbstract: Little is known about the muscle developmental patterns during embryonic to neonatal development in ducks. We investigated the developmental patterns in the lateral gastrocnemius muscles of Gaoyou and Jinding ducks differing in their muscle growth rates during the final stages of egg incubation and the first week after hatching. Expression of the MyoD gene was quantified by quantitative real-time PCR (qRT-PCR). The average cross-sectional area and diameter of the fibers increased from embryonic day 21 (E21), peaking at E27, and then declining slightly 7 d after hatching. The density of the fibers decreased initially but increased after hatching in both breeds and sexes. The within-breed variation in muscle fiber-type composition was greater than the average variation between the breeds. Overall, the percentage of type I fibers increased and that of type IIb fibers decreased consistently. However, the percentage of type IIa fibers was almost constant as development proceeded in both duck breeds. The profiles of MyoD mRNA expression were similar in both breeds, and a significantly positive relationship was observed between the expression of MyoD and the percentage of type IIb fibers. This study firstly revealed the characteristics of duck muscle development and differences between the two breeds differing in growth rates. Moreover, type IIb fibers might convert to type I fibers in the lateral gastrocnemius, while MyoD may potentially function in controlling the muscle fiber phenotype during the secondary myogenesis of muscle development.http://www.sciencedirect.com/science/article/pii/S2095311914609497duckmyofiber developmentMyoD genegene expression |
| spellingShingle | Hui-fang LI Jing-ting SHU Yan-ju SHAN Wen-feng CHEN Chi SONG Wen-juan XU Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates Journal of Integrative Agriculture duck myofiber development MyoD gene gene expression |
| title | Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| title_full | Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| title_fullStr | Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| title_full_unstemmed | Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| title_short | Myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| title_sort | myofiber development during embryonic to neonatal development in duck breeds differing in muscle growth rates |
| topic | duck myofiber development MyoD gene gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914609497 |
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