Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase
Haemophilus influenzae is a common organism of the human upper respiratory tract; this bacterium is responsible of a wide spectrum for respiratory infections and can generate invasive diseases such as meningitis and septicemia. These infections are associated with H. influenzae encapsulated serotype...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/6629824 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560665517096960 |
|---|---|
| author | Yesenia Osorio-Aguilar Maria Cristina Gonzalez-Vazquez Patricia Lozano-Zarain Ygnacio Martinez-Laguna Alejandro Carabarin-Lima Rosa del Carmen Rocha-Gracia |
| author_facet | Yesenia Osorio-Aguilar Maria Cristina Gonzalez-Vazquez Patricia Lozano-Zarain Ygnacio Martinez-Laguna Alejandro Carabarin-Lima Rosa del Carmen Rocha-Gracia |
| author_sort | Yesenia Osorio-Aguilar |
| collection | DOAJ |
| description | Haemophilus influenzae is a common organism of the human upper respiratory tract; this bacterium is responsible of a wide spectrum for respiratory infections and can generate invasive diseases such as meningitis and septicemia. These infections are associated with H. influenzae encapsulated serotype b. However, the incidence of invasive disease caused by nontypeable H. influenzae (NTHi) has increased in the post-H. influenzae serotype b (Hib) vaccine era. Currently, an effective vaccine against NTHi is not available; due to this, it is important to find an antigen capable to confer protection against NTHi infection. In this study, 10 linear B cell epitopes and 13 CTL epitopes and a putative plasminogen-binding motif (252FYNKENGMY260) and the presence of enolase on the surface of different strains of H. influenzae were identified in the enolase sequence of H. influenzae. Both in silico and experimental results showed that recombinant enolase from H. influenzae is immunogenic that could induce a humoral immune response; this was observed mediating the generation of specific polyclonal antibodies anti-rNTHiENO that recognize typeable and nontypeable H. influenzae strains. The immunogenic properties and the superficial localization of enolase in H. influenzae, important characteristics to be considered as a new candidate for the development of a vaccine, were demonstrated. |
| format | Article |
| id | doaj-art-547bb5f83e4846d4826f4672c014c344 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-547bb5f83e4846d4826f4672c014c3442025-02-03T01:26:58ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/66298246629824Cloning and Characterization of Immunological Properties of Haemophilus influenzae EnolaseYesenia Osorio-Aguilar0Maria Cristina Gonzalez-Vazquez1Patricia Lozano-Zarain2Ygnacio Martinez-Laguna3Alejandro Carabarin-Lima4Rosa del Carmen Rocha-Gracia5Benemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoBenemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoBenemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoBenemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoBenemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoBenemerita Universidad Autonoma de Puebla, Instituto de Ciencias, Centro de Investigaciones en Ciencias Microbiologicas, Posgrado en Microbiologia, Puebla, Puebla, MexicoHaemophilus influenzae is a common organism of the human upper respiratory tract; this bacterium is responsible of a wide spectrum for respiratory infections and can generate invasive diseases such as meningitis and septicemia. These infections are associated with H. influenzae encapsulated serotype b. However, the incidence of invasive disease caused by nontypeable H. influenzae (NTHi) has increased in the post-H. influenzae serotype b (Hib) vaccine era. Currently, an effective vaccine against NTHi is not available; due to this, it is important to find an antigen capable to confer protection against NTHi infection. In this study, 10 linear B cell epitopes and 13 CTL epitopes and a putative plasminogen-binding motif (252FYNKENGMY260) and the presence of enolase on the surface of different strains of H. influenzae were identified in the enolase sequence of H. influenzae. Both in silico and experimental results showed that recombinant enolase from H. influenzae is immunogenic that could induce a humoral immune response; this was observed mediating the generation of specific polyclonal antibodies anti-rNTHiENO that recognize typeable and nontypeable H. influenzae strains. The immunogenic properties and the superficial localization of enolase in H. influenzae, important characteristics to be considered as a new candidate for the development of a vaccine, were demonstrated.http://dx.doi.org/10.1155/2021/6629824 |
| spellingShingle | Yesenia Osorio-Aguilar Maria Cristina Gonzalez-Vazquez Patricia Lozano-Zarain Ygnacio Martinez-Laguna Alejandro Carabarin-Lima Rosa del Carmen Rocha-Gracia Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase Journal of Immunology Research |
| title | Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase |
| title_full | Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase |
| title_fullStr | Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase |
| title_full_unstemmed | Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase |
| title_short | Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase |
| title_sort | cloning and characterization of immunological properties of haemophilus influenzae enolase |
| url | http://dx.doi.org/10.1155/2021/6629824 |
| work_keys_str_mv | AT yeseniaosorioaguilar cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase AT mariacristinagonzalezvazquez cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase AT patricialozanozarain cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase AT ygnaciomartinezlaguna cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase AT alejandrocarabarinlima cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase AT rosadelcarmenrochagracia cloningandcharacterizationofimmunologicalpropertiesofhaemophilusinfluenzaeenolase |