Protocol for measuring the auxin-induced changes of m6A modification
Summary: Plant m6A modification is highly dynamic, with plant hormones serving as key regulators that induce these dynamics. Here, we present a protocol for detecting phytohormone-induced changes in m6A modification using RNA dot blot and m6A sequencing (m6A-seq) techniques. We describe steps for th...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000358 |
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| author | Bin Li Yixing Li Lei Li Linjun Cai Lan Li Li Li Chongsheng He |
| author_facet | Bin Li Yixing Li Lei Li Linjun Cai Lan Li Li Li Chongsheng He |
| author_sort | Bin Li |
| collection | DOAJ |
| description | Summary: Plant m6A modification is highly dynamic, with plant hormones serving as key regulators that induce these dynamics. Here, we present a protocol for detecting phytohormone-induced changes in m6A modification using RNA dot blot and m6A sequencing (m6A-seq) techniques. We describe steps for the auxin treatment, RNA dot blot and m6A-seq library preparation, and data analysis. This protocol holds potential applications for analyzing changes in m6A modification induced by plant hormones.For complete details on the use and execution of this protocol, please refer to Bin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-5454c1a3b4ef42cfa8a7d2f7335fc2e5 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-5454c1a3b4ef42cfa8a7d2f7335fc2e52025-02-06T05:12:49ZengElsevierSTAR Protocols2666-16672025-03-0161103629Protocol for measuring the auxin-induced changes of m6A modificationBin Li0Yixing Li1Lei Li2Linjun Cai3Lan Li4Li Li5Chongsheng He6State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410082, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410082, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410082, ChinaCollege of Biology, Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, Hunan 410082, ChinaSchool of Pharmacy, Xiangnan University, Chenzhou, Hunan 423000, China; Corresponding authorState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410082, China; Corresponding authorCollege of Biology, Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, Hunan 410082, China; Corresponding authorSummary: Plant m6A modification is highly dynamic, with plant hormones serving as key regulators that induce these dynamics. Here, we present a protocol for detecting phytohormone-induced changes in m6A modification using RNA dot blot and m6A sequencing (m6A-seq) techniques. We describe steps for the auxin treatment, RNA dot blot and m6A-seq library preparation, and data analysis. This protocol holds potential applications for analyzing changes in m6A modification induced by plant hormones.For complete details on the use and execution of this protocol, please refer to Bin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000358Developmental biologyGeneticsPlant sciences |
| spellingShingle | Bin Li Yixing Li Lei Li Linjun Cai Lan Li Li Li Chongsheng He Protocol for measuring the auxin-induced changes of m6A modification STAR Protocols Developmental biology Genetics Plant sciences |
| title | Protocol for measuring the auxin-induced changes of m6A modification |
| title_full | Protocol for measuring the auxin-induced changes of m6A modification |
| title_fullStr | Protocol for measuring the auxin-induced changes of m6A modification |
| title_full_unstemmed | Protocol for measuring the auxin-induced changes of m6A modification |
| title_short | Protocol for measuring the auxin-induced changes of m6A modification |
| title_sort | protocol for measuring the auxin induced changes of m6a modification |
| topic | Developmental biology Genetics Plant sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000358 |
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