Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis
This study investigated the impacts of GLN on inflammation and T cell dysregulation in obese mice complicated with sepsis. Mice were divided into normal control (NC) and high-fat diet groups. The high-fat diet provided 60% of energy from fat and was administered for 10 weeks to induce obesity. Mice...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8869017 |
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| author | Chiu-Li Yeh Li-Han Su Jin-Ming Wu Po-Jen Yang Po-Chu Lee Po-Da Chen Chun-Chieh Huang Der-Yirng Hsieh Hsueh-Ju Wang Sung-Ling Yeh Ming-Tsan Lin |
| author_facet | Chiu-Li Yeh Li-Han Su Jin-Ming Wu Po-Jen Yang Po-Chu Lee Po-Da Chen Chun-Chieh Huang Der-Yirng Hsieh Hsueh-Ju Wang Sung-Ling Yeh Ming-Tsan Lin |
| author_sort | Chiu-Li Yeh |
| collection | DOAJ |
| description | This study investigated the impacts of GLN on inflammation and T cell dysregulation in obese mice complicated with sepsis. Mice were divided into normal control (NC) and high-fat diet groups. The high-fat diet provided 60% of energy from fat and was administered for 10 weeks to induce obesity. Mice fed with a high-fat diet were then assigned to sham (SH) and sepsis with saline (SS) or GLN (SG) groups. The SH group was subjected to laparotomy, while the sepsis group underwent cecal ligation and puncture (CLP). The SS group was intravenously injected with saline. The SG group was intravenously administered GLN after CLP. Mice were sacrificed at 12, 24, or 48 h post-CLP, respectively. Results demonstrated that in the presence of obesity, sepsis drove CD4+ T cells toward the helper T (Th)2 and Th17 lineages. Also, expressions of inflammatory cytokines and macrophage infiltration markers in adipose tissues and lungs were elevated. Treatment of obese mice with GLN after sepsis reversed Th polarization and downregulated macrophage infiltration and inflammatory cytokine, whereas the tight junction-associated protein expression increased in the lungs. These findings suggest that the intravenous administration of GLN to obese mice after sepsis modulated a more balanced Th cell lineage, alleviated inflammation, and attenuated lung injury. |
| format | Article |
| id | doaj-art-545481dcab8644c3bef010f8b5d658d9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-545481dcab8644c3bef010f8b5d658d92025-02-03T05:57:51ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/88690178869017Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial SepsisChiu-Li Yeh0Li-Han Su1Jin-Ming Wu2Po-Jen Yang3Po-Chu Lee4Po-Da Chen5Chun-Chieh Huang6Der-Yirng Hsieh7Hsueh-Ju Wang8Sung-Ling Yeh9Ming-Tsan Lin10School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Nursing, Nutrition Support Team, National Taiwan University Hospital, Taipei, TaiwanDepartment of Pharmacy, National Taiwan University Hospital, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, TaiwanThis study investigated the impacts of GLN on inflammation and T cell dysregulation in obese mice complicated with sepsis. Mice were divided into normal control (NC) and high-fat diet groups. The high-fat diet provided 60% of energy from fat and was administered for 10 weeks to induce obesity. Mice fed with a high-fat diet were then assigned to sham (SH) and sepsis with saline (SS) or GLN (SG) groups. The SH group was subjected to laparotomy, while the sepsis group underwent cecal ligation and puncture (CLP). The SS group was intravenously injected with saline. The SG group was intravenously administered GLN after CLP. Mice were sacrificed at 12, 24, or 48 h post-CLP, respectively. Results demonstrated that in the presence of obesity, sepsis drove CD4+ T cells toward the helper T (Th)2 and Th17 lineages. Also, expressions of inflammatory cytokines and macrophage infiltration markers in adipose tissues and lungs were elevated. Treatment of obese mice with GLN after sepsis reversed Th polarization and downregulated macrophage infiltration and inflammatory cytokine, whereas the tight junction-associated protein expression increased in the lungs. These findings suggest that the intravenous administration of GLN to obese mice after sepsis modulated a more balanced Th cell lineage, alleviated inflammation, and attenuated lung injury.http://dx.doi.org/10.1155/2020/8869017 |
| spellingShingle | Chiu-Li Yeh Li-Han Su Jin-Ming Wu Po-Jen Yang Po-Chu Lee Po-Da Chen Chun-Chieh Huang Der-Yirng Hsieh Hsueh-Ju Wang Sung-Ling Yeh Ming-Tsan Lin Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis Mediators of Inflammation |
| title | Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis |
| title_full | Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis |
| title_fullStr | Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis |
| title_full_unstemmed | Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis |
| title_short | Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis |
| title_sort | effects of the glutamine administration on t helper cell regulation and inflammatory response in obese mice complicated with polymicrobial sepsis |
| url | http://dx.doi.org/10.1155/2020/8869017 |
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