A guide for the generation of repositories of clinical samples for research on Chagas disease.
Chagas disease, caused by the parasite Trypanosoma cruzi, affects over 6 million people, mainly in Latin America. Two different clinical phases, acute and chronic, are recognised. Currently, 2 anti-parasitic drugs are available to treat the disease (nifurtimox and benznidazole), but diagnostic metho...
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Public Library of Science (PLoS)
2024-08-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0012166 |
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| author | Nieves Martínez-Peinado Juan Carlos Gabaldón-Figueira Roberto Rodrigues Ferreira María Carmen Thomas Manuel Carlos López Tania Cremonini Araújo-Jorge Belkisyolé Alarcón de Noya Soledad Berón Janine Ramsey Irene Losada Galván Alejandro Gabriel Schijman Adriana González Martínez Andrés Mariano Ruiz Gimena Rojas Roberto Magalhães Saraiva Oscar Noya-González Andrea Gómez Rosa A Maldonado Jimmy Pinto Faustino Torrico Ivan Scandale Fernán Agüero María-Jesús Pinazo Joaquim Gascón Alejandro Marcel Hasslocher-Moreno Julio Alonso-Padilla NHEPACHA Network (New Tools for the Diagnosis and Evaluation of Chagas Disease) |
| author_facet | Nieves Martínez-Peinado Juan Carlos Gabaldón-Figueira Roberto Rodrigues Ferreira María Carmen Thomas Manuel Carlos López Tania Cremonini Araújo-Jorge Belkisyolé Alarcón de Noya Soledad Berón Janine Ramsey Irene Losada Galván Alejandro Gabriel Schijman Adriana González Martínez Andrés Mariano Ruiz Gimena Rojas Roberto Magalhães Saraiva Oscar Noya-González Andrea Gómez Rosa A Maldonado Jimmy Pinto Faustino Torrico Ivan Scandale Fernán Agüero María-Jesús Pinazo Joaquim Gascón Alejandro Marcel Hasslocher-Moreno Julio Alonso-Padilla NHEPACHA Network (New Tools for the Diagnosis and Evaluation of Chagas Disease) |
| author_sort | Nieves Martínez-Peinado |
| collection | DOAJ |
| description | Chagas disease, caused by the parasite Trypanosoma cruzi, affects over 6 million people, mainly in Latin America. Two different clinical phases, acute and chronic, are recognised. Currently, 2 anti-parasitic drugs are available to treat the disease (nifurtimox and benznidazole), but diagnostic methods require of a relatively complex infrastructure and trained personnel, limiting its widespread use in endemic areas, and the access of patients to treatment. New diagnostic methods, such as rapid tests (RDTs) to diagnose chronic Chagas disease, or loop-mediated isothermal amplification (LAMP), to detect acute infections, represent valuable alternatives, but the parasite's remarkable genetic diversity might make its implementation difficult. Furthermore, determining the efficacy of Chagas disease treatment is complicated, given the slow reversion of serological anti-T. cruzi antibody reactivity, which may even take decades to occur. New biomarkers to evaluate early therapeutic efficacy, as well as diagnostic tests able to detect the wide variety of circulating genotypes, are therefore, urgently required. To carry out studies that address these needs, high-quality and traceable samples from T. cruzi-infected individuals with different geographical backgrounds, along with associated clinical and epidemiological data, are necessary. This work describes the framework for the creation of such repositories, following standardised and uniform protocols, and considering the ethical, technical, and logistic aspects of the process. The manual can be adapted according to the resources of each laboratory, to guarantee that samples are obtained in a reproducible way, favouring the exchange of data among different work groups, and their generalizable evaluation and analysis. The main objective of this is to accelerate the development of new diagnostic methods and the identification of biomarkers for Chagas disease. |
| format | Article |
| id | doaj-art-542e8f3288c8497496fa6fd2380b1886 |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-542e8f3288c8497496fa6fd2380b18862025-02-05T05:33:31ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352024-08-01188e001216610.1371/journal.pntd.0012166A guide for the generation of repositories of clinical samples for research on Chagas disease.Nieves Martínez-PeinadoJuan Carlos Gabaldón-FigueiraRoberto Rodrigues FerreiraMaría Carmen ThomasManuel Carlos LópezTania Cremonini Araújo-JorgeBelkisyolé Alarcón de NoyaSoledad BerónJanine RamseyIrene Losada GalvánAlejandro Gabriel SchijmanAdriana González MartínezAndrés Mariano RuizGimena RojasRoberto Magalhães SaraivaOscar Noya-GonzálezAndrea GómezRosa A MaldonadoJimmy PintoFaustino TorricoIvan ScandaleFernán AgüeroMaría-Jesús PinazoJoaquim GascónAlejandro Marcel Hasslocher-MorenoJulio Alonso-PadillaNHEPACHA Network (New Tools for the Diagnosis and Evaluation of Chagas Disease)Chagas disease, caused by the parasite Trypanosoma cruzi, affects over 6 million people, mainly in Latin America. Two different clinical phases, acute and chronic, are recognised. Currently, 2 anti-parasitic drugs are available to treat the disease (nifurtimox and benznidazole), but diagnostic methods require of a relatively complex infrastructure and trained personnel, limiting its widespread use in endemic areas, and the access of patients to treatment. New diagnostic methods, such as rapid tests (RDTs) to diagnose chronic Chagas disease, or loop-mediated isothermal amplification (LAMP), to detect acute infections, represent valuable alternatives, but the parasite's remarkable genetic diversity might make its implementation difficult. Furthermore, determining the efficacy of Chagas disease treatment is complicated, given the slow reversion of serological anti-T. cruzi antibody reactivity, which may even take decades to occur. New biomarkers to evaluate early therapeutic efficacy, as well as diagnostic tests able to detect the wide variety of circulating genotypes, are therefore, urgently required. To carry out studies that address these needs, high-quality and traceable samples from T. cruzi-infected individuals with different geographical backgrounds, along with associated clinical and epidemiological data, are necessary. This work describes the framework for the creation of such repositories, following standardised and uniform protocols, and considering the ethical, technical, and logistic aspects of the process. The manual can be adapted according to the resources of each laboratory, to guarantee that samples are obtained in a reproducible way, favouring the exchange of data among different work groups, and their generalizable evaluation and analysis. The main objective of this is to accelerate the development of new diagnostic methods and the identification of biomarkers for Chagas disease.https://doi.org/10.1371/journal.pntd.0012166 |
| spellingShingle | Nieves Martínez-Peinado Juan Carlos Gabaldón-Figueira Roberto Rodrigues Ferreira María Carmen Thomas Manuel Carlos López Tania Cremonini Araújo-Jorge Belkisyolé Alarcón de Noya Soledad Berón Janine Ramsey Irene Losada Galván Alejandro Gabriel Schijman Adriana González Martínez Andrés Mariano Ruiz Gimena Rojas Roberto Magalhães Saraiva Oscar Noya-González Andrea Gómez Rosa A Maldonado Jimmy Pinto Faustino Torrico Ivan Scandale Fernán Agüero María-Jesús Pinazo Joaquim Gascón Alejandro Marcel Hasslocher-Moreno Julio Alonso-Padilla NHEPACHA Network (New Tools for the Diagnosis and Evaluation of Chagas Disease) A guide for the generation of repositories of clinical samples for research on Chagas disease. PLoS Neglected Tropical Diseases |
| title | A guide for the generation of repositories of clinical samples for research on Chagas disease. |
| title_full | A guide for the generation of repositories of clinical samples for research on Chagas disease. |
| title_fullStr | A guide for the generation of repositories of clinical samples for research on Chagas disease. |
| title_full_unstemmed | A guide for the generation of repositories of clinical samples for research on Chagas disease. |
| title_short | A guide for the generation of repositories of clinical samples for research on Chagas disease. |
| title_sort | guide for the generation of repositories of clinical samples for research on chagas disease |
| url | https://doi.org/10.1371/journal.pntd.0012166 |
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