Protocol for dual-optical mapping of voltage and calcium sensors in human pluripotent stem cell-derived cardiomyocytes

Protocol for dual-optical mapping of voltage and calcium sensors in human pluripotent stem cell-derived cardiomyocytes

Summary: Simultaneous acquisition of fluorescent voltage and intracellular calcium signals requires spectrally distinct indicators and a high-speed imaging setup. Here, we present a protocol for the optical mapping of these key physiological parameters in human pluripotent stem cell-derived cardiomy...

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Bibliographic Details
Main Authors: Faisal J. Alibhai, Arya Masoumi, Ho-June Kim, Nathan Kim, Wesley Kim, Juliana Gomez-Garcia, Peter Lee, Michael A. Laflamme
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Health Sciences
Molecular/Chemical Probes
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004502
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