Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines
Carcinogenesis is related to the loss of homeostatic control of cellular processes regulated by transcriptional circuits and epigenetic mechanisms. Among these, the activities of peroxisome proliferator-activated receptors (PPARs) and DNA methyltransferases (DNMTs) are crucial and intertwined. PPARγ...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2012/890875 |
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| author | Valerio Pazienza Francesca Tavano Massimo Francavilla Andrea Fontana Fabio Pellegrini Giorgia Benegiamo Vincenzo Corbo Fabio Francesco di Mola Pierluigi Di Sebastiano Angelo Andriulli Gianluigi Mazzoccoli |
| author_facet | Valerio Pazienza Francesca Tavano Massimo Francavilla Andrea Fontana Fabio Pellegrini Giorgia Benegiamo Vincenzo Corbo Fabio Francesco di Mola Pierluigi Di Sebastiano Angelo Andriulli Gianluigi Mazzoccoli |
| author_sort | Valerio Pazienza |
| collection | DOAJ |
| description | Carcinogenesis is related to the loss of homeostatic control of cellular processes regulated by transcriptional circuits and epigenetic mechanisms. Among these, the activities of peroxisome proliferator-activated receptors (PPARs) and DNA methyltransferases (DNMTs) are crucial and intertwined. PPARγ is a key regulator of cell fate, linking nutrient sensing to transcription processes, and its expression oscillates with circadian rhythmicity. Aim of our study was to assess the periodicity of PPARγ and DNMTs in pancreatic cancer (PC). We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. PPARG and DNMT1 expression in PANC-1 cells and PPARG expression in MIAPaCa-2 cells were characterized by a 24 h period oscillation, and a borderline significant rhythm was observed for the PPARG, DNMT1, and DNMT3B expression profiles in the other cell lines. The time-qualified profiles of gene expression showed different shapes and phase relationships in the PC cell lines examined. In conclusion, PPARG and DNMTs expression is characterized by different time-qualified patterns in cell lines derived from human PC, and this heterogeneity could influence cell phenotype and human disease behaviour. |
| format | Article |
| id | doaj-art-53c7ffbe2f754221940ade8e0edb02e7 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-53c7ffbe2f754221940ade8e0edb02e72025-02-03T01:30:08ZengWileyPPAR Research1687-47571687-47652012-01-01201210.1155/2012/890875890875Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell LinesValerio Pazienza0Francesca Tavano1Massimo Francavilla2Andrea Fontana3Fabio Pellegrini4Giorgia Benegiamo5Vincenzo Corbo6Fabio Francesco di Mola7Pierluigi Di Sebastiano8Angelo Andriulli9Gianluigi Mazzoccoli10Research Laboratory and Gastroenterology Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyResearch Laboratory and Surgery Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyComputing Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyUnit of Biostatistics, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyUnit of Biostatistics, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyResearch Laboratory and Gastroenterology Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyARC-NET Miriam Cherubini Centre for Applied Research on Cancer, University and Hospital Trust of Verona, 37100 Verona, ItalyResearch Laboratory and Surgery Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyResearch Laboratory and Surgery Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyResearch Laboratory and Gastroenterology Unit, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyDivision of Internal Medicine and Chronobiology Unit, Department of Medical Sciences, Scientific Institute and Regional General Hospital “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo, ItalyCarcinogenesis is related to the loss of homeostatic control of cellular processes regulated by transcriptional circuits and epigenetic mechanisms. Among these, the activities of peroxisome proliferator-activated receptors (PPARs) and DNA methyltransferases (DNMTs) are crucial and intertwined. PPARγ is a key regulator of cell fate, linking nutrient sensing to transcription processes, and its expression oscillates with circadian rhythmicity. Aim of our study was to assess the periodicity of PPARγ and DNMTs in pancreatic cancer (PC). We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. PPARG and DNMT1 expression in PANC-1 cells and PPARG expression in MIAPaCa-2 cells were characterized by a 24 h period oscillation, and a borderline significant rhythm was observed for the PPARG, DNMT1, and DNMT3B expression profiles in the other cell lines. The time-qualified profiles of gene expression showed different shapes and phase relationships in the PC cell lines examined. In conclusion, PPARG and DNMTs expression is characterized by different time-qualified patterns in cell lines derived from human PC, and this heterogeneity could influence cell phenotype and human disease behaviour.http://dx.doi.org/10.1155/2012/890875 |
| spellingShingle | Valerio Pazienza Francesca Tavano Massimo Francavilla Andrea Fontana Fabio Pellegrini Giorgia Benegiamo Vincenzo Corbo Fabio Francesco di Mola Pierluigi Di Sebastiano Angelo Andriulli Gianluigi Mazzoccoli Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines PPAR Research |
| title | Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines |
| title_full | Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines |
| title_fullStr | Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines |
| title_full_unstemmed | Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines |
| title_short | Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines |
| title_sort | time qualified patterns of variation of pparγ dnmt1 and dnmt3b expression in pancreatic cancer cell lines |
| url | http://dx.doi.org/10.1155/2012/890875 |
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