L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation
L-theanine, a non-protein amino acid naturally occurring in tea leaves, is recognized for its antioxidant, anti-inflammatory, and neuroprotective properties. Despite its known benefits, the mechanisms by which L-theanine influences lifespan extension remain poorly understood. This study investigated...
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MDPI AG
2025-01-01
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| author | Zhihang Huang Haiming Jing Yan Pan Hongxia Cai Wenjing Zhang Jingyuan Zhu Nan Zhang Dan Wu Wentao Xu Hexiang Qiu Huihui Bao Guojun Li Junyu Ning Bo Xian Shan Gao |
| author_facet | Zhihang Huang Haiming Jing Yan Pan Hongxia Cai Wenjing Zhang Jingyuan Zhu Nan Zhang Dan Wu Wentao Xu Hexiang Qiu Huihui Bao Guojun Li Junyu Ning Bo Xian Shan Gao |
| author_sort | Zhihang Huang |
| collection | DOAJ |
| description | L-theanine, a non-protein amino acid naturally occurring in tea leaves, is recognized for its antioxidant, anti-inflammatory, and neuroprotective properties. Despite its known benefits, the mechanisms by which L-theanine influences lifespan extension remain poorly understood. This study investigated the effects of L-theanine on the lifespan of <i>Caenorhabditis elegans</i> and explored the underlying mechanisms. Our findings indicate that L-theanine significantly diminishes the accumulation of advanced glycation end products (AGEs), which are biomarkers closely linked to aging and age-related diseases. Through an AGE-level analysis, we observed that L-theanine, when administered during early adulthood, notably extended the lifespan of <i>Caenorhabditis elegans</i> under both normal and high-glucose-induced stress conditions. L-theanine enhanced the lifespan under typical conditions and provided protective effects against high-glucose-induced stress. A further analysis demonstrated that L-theanine extends the lifespan of <i>Caenorhabditis elegans</i> by modulating the DAF-2/DAF-16 insulin-like signaling pathway and reducing the accumulation of advanced glycation end products (AGEs). In summary, this study identified L-theanine as a potential anti-aging intervention that extends the lifespan by reducing AGE accumulation and regulating insulin-like signaling pathways. These findings provide new insights for developing anti-aging strategies and lay the groundwork for further research on the potential benefits of L-theanine in mammals. Future studies could explore the molecular mechanisms, test L-theanine in mammalian models, and assess the long-term side effects. |
| format | Article |
| id | doaj-art-53bdb10f6b9149f0a0bd986099278f15 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-53bdb10f6b9149f0a0bd986099278f152025-01-24T13:32:55ZengMDPI AGFoods2304-81582025-01-0114222110.3390/foods14020221L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced GlycosylationZhihang Huang0Haiming Jing1Yan Pan2Hongxia Cai3Wenjing Zhang4Jingyuan Zhu5Nan Zhang6Dan Wu7Wentao Xu8Hexiang Qiu9Huihui Bao10Guojun Li11Junyu Ning12Bo Xian13Shan Gao14Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaNHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit, China National Center for Food Safety Risk Assessment, Beijing 100022, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaLaboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610056, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaL-theanine, a non-protein amino acid naturally occurring in tea leaves, is recognized for its antioxidant, anti-inflammatory, and neuroprotective properties. Despite its known benefits, the mechanisms by which L-theanine influences lifespan extension remain poorly understood. This study investigated the effects of L-theanine on the lifespan of <i>Caenorhabditis elegans</i> and explored the underlying mechanisms. Our findings indicate that L-theanine significantly diminishes the accumulation of advanced glycation end products (AGEs), which are biomarkers closely linked to aging and age-related diseases. Through an AGE-level analysis, we observed that L-theanine, when administered during early adulthood, notably extended the lifespan of <i>Caenorhabditis elegans</i> under both normal and high-glucose-induced stress conditions. L-theanine enhanced the lifespan under typical conditions and provided protective effects against high-glucose-induced stress. A further analysis demonstrated that L-theanine extends the lifespan of <i>Caenorhabditis elegans</i> by modulating the DAF-2/DAF-16 insulin-like signaling pathway and reducing the accumulation of advanced glycation end products (AGEs). In summary, this study identified L-theanine as a potential anti-aging intervention that extends the lifespan by reducing AGE accumulation and regulating insulin-like signaling pathways. These findings provide new insights for developing anti-aging strategies and lay the groundwork for further research on the potential benefits of L-theanine in mammals. Future studies could explore the molecular mechanisms, test L-theanine in mammalian models, and assess the long-term side effects.https://www.mdpi.com/2304-8158/14/2/221L-theanine<i>Caenorhabditis elegans</i>advanced glycation end products (AGEs)lifespan extensioninsulin-like signaling |
| spellingShingle | Zhihang Huang Haiming Jing Yan Pan Hongxia Cai Wenjing Zhang Jingyuan Zhu Nan Zhang Dan Wu Wentao Xu Hexiang Qiu Huihui Bao Guojun Li Junyu Ning Bo Xian Shan Gao L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation Foods L-theanine <i>Caenorhabditis elegans</i> advanced glycation end products (AGEs) lifespan extension insulin-like signaling |
| title | L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation |
| title_full | L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation |
| title_fullStr | L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation |
| title_full_unstemmed | L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation |
| title_short | L-Theanine Extends the Lifespan of <i>Caenorhabditis elegans</i> by Reducing the End Products of Advanced Glycosylation |
| title_sort | l theanine extends the lifespan of i caenorhabditis elegans i by reducing the end products of advanced glycosylation |
| topic | L-theanine <i>Caenorhabditis elegans</i> advanced glycation end products (AGEs) lifespan extension insulin-like signaling |
| url | https://www.mdpi.com/2304-8158/14/2/221 |
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