Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates
Obesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health, but its effects on m...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2018-12-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.journalssystem.com/pjfns/,100426,0,2.html |
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| author | Diana Olguín-Calderón Jorge L. González-Escobar Rosalva Ríos-Villa Elena Dibildox-Alvarado Antonio De León-Rodríguez Ana P. Barba de la Rosa |
| author_facet | Diana Olguín-Calderón Jorge L. González-Escobar Rosalva Ríos-Villa Elena Dibildox-Alvarado Antonio De León-Rodríguez Ana P. Barba de la Rosa |
| author_sort | Diana Olguín-Calderón |
| collection | DOAJ |
| description | Obesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health, but its effects on microbiota profile are still unknown. The aim of this study was to analyse the changes of gut microbiota in diet-induced obese mice due to amaranth protein consumption and to compare them with the changes due to soybean protein intake. Male C57BL/6 mice were fed for 8 weeks with regular (RD) or high-fat (HF) diet, without or with complementation with amaranth or soybean protein isolates. Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCFAs) were quantified by gas chromatography. The consumption of soybean protein induced the ectopic deposition of fat in the whole intestine while amaranth proteins increased caecal crypt depth and calceiform cells number sustaining its beneficial effect on health. The count of Ruminococcacea family bacteria was increased in mice fed with HF diet, but amaranth proteins intake reduced its abundance. In turn, Lachnospiraceae bacteria abundance decreased in mice fed the Control-HF and amaranth HF diets, but increased in mice fed the soybean diets. In mice fed the RD diets, amaranth induced the abundance of Prevotellaceae, an acetate-producing bacteria. Study results indicate that the modulation of caecal microbiota could be one of the mechanisms by which amaranth exerts its beneficial effects on health. |
| format | Article |
| id | doaj-art-5394f2d5f0c44d65b81a8fa79ae4afa8 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-5394f2d5f0c44d65b81a8fa79ae4afa82025-02-02T03:39:47ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072018-12-01691354410.31883/pjfns-2019-0002100426Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein IsolatesDiana Olguín-Calderón0Jorge L. González-Escobar1Rosalva Ríos-Villa2Elena Dibildox-Alvarado3Antonio De León-Rodríguez4Ana P. Barba de la Rosa5IPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí, S.L.P., 78216 MexicoIPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí, S.L.P., 78216 MexicoIPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí, S.L.P., 78216 MexicoFacultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí. Av. Dr. Salvador Nava 6, Zona Universitaria, San Luis Potosí, S.L.P., 78210 MexicoIPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí, S.L.P., 78216 MexicoIPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí, S.L.P., 78216 MexicoObesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health, but its effects on microbiota profile are still unknown. The aim of this study was to analyse the changes of gut microbiota in diet-induced obese mice due to amaranth protein consumption and to compare them with the changes due to soybean protein intake. Male C57BL/6 mice were fed for 8 weeks with regular (RD) or high-fat (HF) diet, without or with complementation with amaranth or soybean protein isolates. Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCFAs) were quantified by gas chromatography. The consumption of soybean protein induced the ectopic deposition of fat in the whole intestine while amaranth proteins increased caecal crypt depth and calceiform cells number sustaining its beneficial effect on health. The count of Ruminococcacea family bacteria was increased in mice fed with HF diet, but amaranth proteins intake reduced its abundance. In turn, Lachnospiraceae bacteria abundance decreased in mice fed the Control-HF and amaranth HF diets, but increased in mice fed the soybean diets. In mice fed the RD diets, amaranth induced the abundance of Prevotellaceae, an acetate-producing bacteria. Study results indicate that the modulation of caecal microbiota could be one of the mechanisms by which amaranth exerts its beneficial effects on health.http://www.journalssystem.com/pjfns/,100426,0,2.htmlamaranthcaecal microbiomeobesityprotein intakeshort-chain fatty acids |
| spellingShingle | Diana Olguín-Calderón Jorge L. González-Escobar Rosalva Ríos-Villa Elena Dibildox-Alvarado Antonio De León-Rodríguez Ana P. Barba de la Rosa Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates Polish Journal of Food and Nutrition Sciences amaranth caecal microbiome obesity protein intake short-chain fatty acids |
| title | Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates |
| title_full | Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates |
| title_fullStr | Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates |
| title_full_unstemmed | Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates |
| title_short | Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates |
| title_sort | modulation of caecal microbiome in obese mice associated with administration of amaranth or soybean protein isolates |
| topic | amaranth caecal microbiome obesity protein intake short-chain fatty acids |
| url | http://www.journalssystem.com/pjfns/,100426,0,2.html |
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