Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco
Pomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels an...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2020/8885889 |
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| author | Talal Sabraoui Taleb Khider Boubker Nasser Rabiaa Eddoha Abderrahman Moujahid Maryam Benbachir Abdelkhalid Essamadi |
| author_facet | Talal Sabraoui Taleb Khider Boubker Nasser Rabiaa Eddoha Abderrahman Moujahid Maryam Benbachir Abdelkhalid Essamadi |
| author_sort | Talal Sabraoui |
| collection | DOAJ |
| description | Pomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels and seeds obtained from three Moroccan provinces, using UHPLC-DAD. In addition, total phenolic content (TPC), total flavonoid contents (TFC), and metal chelating of pomegranate peel were also evaluated. The results showed that pomegranate peel possesses the highest phenolic (TPC: 224.39 mg GAE/g dw) and flavonoid (TFC: 62.64 mg rutin/g dw) contents. Punicalagin-β and punicalagin-α, are the abundant compounds found in peel: 216.36±9.94 mg/g, 154.94±5.21 mg/g, respectively. Pomegranate peels showed significantly (p<0.05) high antioxidant activity 1-diphenyl-2-picrylhydrazyl (DPPH) EC50: 42.71±0.04 μg/mL, 2.2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) EC50: 62.15±0.01 μg/mL), and chelating activity (FRAP 1.85±0.00 mg ascorbic acid equivalents/100 g, Fe2+: 2.52±0.01 μmol EDTA equivalents/g dw) compared to seeds. A positive correlation between antioxidant activity and total phenolic was found. According to achieved results, high antioxidant capacity of pomegranate extracts, especially peel, shed light to further use as natural food preservatives. Pomegranate peel could be used for the fortification of food with fiber by introducing it in dietary, as well as in health applications due to its higher antioxidant capacity. |
| format | Article |
| id | doaj-art-53949e75cc6b4cc9b720925031c9ebf3 |
| institution | Kabale University |
| issn | 2356-7015 2314-5765 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-53949e75cc6b4cc9b720925031c9ebf32025-02-03T01:28:26ZengWileyInternational Journal of Food Science2356-70152314-57652020-01-01202010.1155/2020/88858898885889Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in MoroccoTalal Sabraoui0Taleb Khider1Boubker Nasser2Rabiaa Eddoha3Abderrahman Moujahid4Maryam Benbachir5Abdelkhalid Essamadi6Faculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoFood Chemistry, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universitât Erlangen-Nürnberg (FAU), Nikolaus-Fiebiger-Straße 10, 91058, GermanyFaculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoFaculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoFaculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoFaculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoFaculty of Sciences and Technology Laboratory of Biochemistry & Neurosciences, Applied Biochemistry and Toxicology Team, Hassan First University, 26000 Settat, MoroccoPomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels and seeds obtained from three Moroccan provinces, using UHPLC-DAD. In addition, total phenolic content (TPC), total flavonoid contents (TFC), and metal chelating of pomegranate peel were also evaluated. The results showed that pomegranate peel possesses the highest phenolic (TPC: 224.39 mg GAE/g dw) and flavonoid (TFC: 62.64 mg rutin/g dw) contents. Punicalagin-β and punicalagin-α, are the abundant compounds found in peel: 216.36±9.94 mg/g, 154.94±5.21 mg/g, respectively. Pomegranate peels showed significantly (p<0.05) high antioxidant activity 1-diphenyl-2-picrylhydrazyl (DPPH) EC50: 42.71±0.04 μg/mL, 2.2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) EC50: 62.15±0.01 μg/mL), and chelating activity (FRAP 1.85±0.00 mg ascorbic acid equivalents/100 g, Fe2+: 2.52±0.01 μmol EDTA equivalents/g dw) compared to seeds. A positive correlation between antioxidant activity and total phenolic was found. According to achieved results, high antioxidant capacity of pomegranate extracts, especially peel, shed light to further use as natural food preservatives. Pomegranate peel could be used for the fortification of food with fiber by introducing it in dietary, as well as in health applications due to its higher antioxidant capacity.http://dx.doi.org/10.1155/2020/8885889 |
| spellingShingle | Talal Sabraoui Taleb Khider Boubker Nasser Rabiaa Eddoha Abderrahman Moujahid Maryam Benbachir Abdelkhalid Essamadi Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco International Journal of Food Science |
| title | Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco |
| title_full | Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco |
| title_fullStr | Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco |
| title_full_unstemmed | Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco |
| title_short | Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco |
| title_sort | determination of punicalagins content metal chelating and antioxidant properties of edible pomegranate punica granatum l peels and seeds grown in morocco |
| url | http://dx.doi.org/10.1155/2020/8885889 |
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