Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats

Objective:. Many studies have shown that blood glucose fluctuations (GFs) are more harmful to patients with diabetes mellitus (DM) than sustained hyperglycemia. However, the effect of GF on electrocardiogram (ECG) parameters and vulnerability to ventricular tachycardia/fibrillation (VT/VF) remains p...

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Main Authors: Li-Da Wu, Feng Li, Chao Wang, Shi-Peng Dang, Feng Xiao, Zhen-Ye Zhang, Jie Zhang, Yu-Min Zhang, Cun-Yu Lu, Ying Liu, Guo-Qiang Zhong, Ling-Ling Qian, Ru-Xing Wang
Format: Article
Language:English
Published: Wolters Kluwer Health/LWW 2023-03-01
Series:Cardiology Discovery
Online Access:http://journals.lww.com/10.1097/CD9.0000000000000055
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author Li-Da Wu
Feng Li
Chao Wang
Shi-Peng Dang
Feng Xiao
Zhen-Ye Zhang
Jie Zhang
Yu-Min Zhang
Cun-Yu Lu
Ying Liu
Guo-Qiang Zhong
Ling-Ling Qian
Ru-Xing Wang
author_facet Li-Da Wu
Feng Li
Chao Wang
Shi-Peng Dang
Feng Xiao
Zhen-Ye Zhang
Jie Zhang
Yu-Min Zhang
Cun-Yu Lu
Ying Liu
Guo-Qiang Zhong
Ling-Ling Qian
Ru-Xing Wang
author_sort Li-Da Wu
collection DOAJ
description Objective:. Many studies have shown that blood glucose fluctuations (GFs) are more harmful to patients with diabetes mellitus (DM) than sustained hyperglycemia. However, the effect of GF on electrocardiogram (ECG) parameters and vulnerability to ventricular tachycardia/fibrillation (VT/VF) remains poorly characterized. This study aimed to assess the effect of GF on ECG parameters and induction of VT/VF in streptozotocin (STZ)-induced diabetic Sprague-Dawley rats. Methods:. Male Sprague-Dawley rats were injected with STZ and randomly divided into 3 treatment groups: controlled STZ-induced diabetic rats (C-STZ) (n = 10); uncontrolled STZ-induced diabetic rats (U-STZ) (n = 10); and STZ-induced diabetic rats with glucose fluctuations (GF-STZ) (n = 10). After 12 weeks, baseline ECG recordings were taken and a VT/VF test was performed with the administration of caffeine and dobutamine. Hematoxylin & eosin and masson staining were used to evaluate pathological cardiac changes after intervention. Results:. No significant difference in heart rate, RR interval, P wave (duration and height), PR segment, PR interval, QRS wave duration, and T wave height was observed among the 3 groups (P > 0.05). Compared with the C-STZ group, the U-STZ and GF-STZ groups both had a longer T wave duration ((62.41 ± 2.38) ms vs. (78.37 ± 4.64) ms and (96.06 ± 4.60) ms, P < 0.05), QT interval ((83.66 ± 2.31) ms vs. (101.75 ± 4.56) ms and (119.14 ± 4.88) ms, P < 0.05), and QTc interval ((77.45 ± 1.36) ms vs. (91.36 ± 3.49) ms and (104.55 ± 3.01) ms, P < 0.05), all of which were longest in the GF-STZ group (P < 0.05). Additionally, the GF-STZ group had the highest VT/VF occurrence and duration and the highest arrhythmia score. Conclusion:. This study revealed GF can significantly prolong the QT interval, QTc interval, and T wave duration, as well as increase vulnerability to VT/VF in rats, which may be an important electrophysiological mechanism of GF-related ventricular arrhythmia.
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spelling doaj-art-537f50a05f38466a9c6edf7dba2d0ff92025-08-20T02:26:09ZengWolters Kluwer Health/LWWCardiology Discovery2096-952X2693-84992023-03-013191510.1097/CD9.0000000000000055202303000-00002Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic RatsLi-Da Wu0Feng Li1Chao Wang2Shi-Peng Dang3Feng Xiao4Zhen-Ye Zhang5Jie Zhang6Yu-Min Zhang7Cun-Yu Lu8Ying Liu9Guo-Qiang Zhong10Ling-Ling Qian11Ru-Xing Wang121 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China2 Department of Cardiology, The First Affiliated Hospital of Nanning Medical University, Nanning, Guangxi 530021, China.1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China2 Department of Cardiology, The First Affiliated Hospital of Nanning Medical University, Nanning, Guangxi 530021, China.1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China1 Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, ChinaObjective:. Many studies have shown that blood glucose fluctuations (GFs) are more harmful to patients with diabetes mellitus (DM) than sustained hyperglycemia. However, the effect of GF on electrocardiogram (ECG) parameters and vulnerability to ventricular tachycardia/fibrillation (VT/VF) remains poorly characterized. This study aimed to assess the effect of GF on ECG parameters and induction of VT/VF in streptozotocin (STZ)-induced diabetic Sprague-Dawley rats. Methods:. Male Sprague-Dawley rats were injected with STZ and randomly divided into 3 treatment groups: controlled STZ-induced diabetic rats (C-STZ) (n = 10); uncontrolled STZ-induced diabetic rats (U-STZ) (n = 10); and STZ-induced diabetic rats with glucose fluctuations (GF-STZ) (n = 10). After 12 weeks, baseline ECG recordings were taken and a VT/VF test was performed with the administration of caffeine and dobutamine. Hematoxylin & eosin and masson staining were used to evaluate pathological cardiac changes after intervention. Results:. No significant difference in heart rate, RR interval, P wave (duration and height), PR segment, PR interval, QRS wave duration, and T wave height was observed among the 3 groups (P > 0.05). Compared with the C-STZ group, the U-STZ and GF-STZ groups both had a longer T wave duration ((62.41 ± 2.38) ms vs. (78.37 ± 4.64) ms and (96.06 ± 4.60) ms, P < 0.05), QT interval ((83.66 ± 2.31) ms vs. (101.75 ± 4.56) ms and (119.14 ± 4.88) ms, P < 0.05), and QTc interval ((77.45 ± 1.36) ms vs. (91.36 ± 3.49) ms and (104.55 ± 3.01) ms, P < 0.05), all of which were longest in the GF-STZ group (P < 0.05). Additionally, the GF-STZ group had the highest VT/VF occurrence and duration and the highest arrhythmia score. Conclusion:. This study revealed GF can significantly prolong the QT interval, QTc interval, and T wave duration, as well as increase vulnerability to VT/VF in rats, which may be an important electrophysiological mechanism of GF-related ventricular arrhythmia.http://journals.lww.com/10.1097/CD9.0000000000000055
spellingShingle Li-Da Wu
Feng Li
Chao Wang
Shi-Peng Dang
Feng Xiao
Zhen-Ye Zhang
Jie Zhang
Yu-Min Zhang
Cun-Yu Lu
Ying Liu
Guo-Qiang Zhong
Ling-Ling Qian
Ru-Xing Wang
Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
Cardiology Discovery
title Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
title_full Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
title_fullStr Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
title_full_unstemmed Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
title_short Effects of Glucose Fluctuations on Electrocardiogram Readings and the Development of Ventricular Arrhythmia in Diabetic Rats
title_sort effects of glucose fluctuations on electrocardiogram readings and the development of ventricular arrhythmia in diabetic rats
url http://journals.lww.com/10.1097/CD9.0000000000000055
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