Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine
Aim. To study the anti-inflammatory and liver regenerative effects of adipose-derived mesenchymal stem cells (ADSCs) on a porcine model of ischemia-reperfusion (IR) and hemihepatectomy. Methods. Eighteen healthy Bama miniature pigs were randomly divided into the sham-operated (sham), untreated IR in...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/2489584 |
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| author | Zhihui Jiao Yajun Ma Xiaoning Liu Yansong Ge Qianzhen Zhang Boyang Liu Hongbin Wang |
| author_facet | Zhihui Jiao Yajun Ma Xiaoning Liu Yansong Ge Qianzhen Zhang Boyang Liu Hongbin Wang |
| author_sort | Zhihui Jiao |
| collection | DOAJ |
| description | Aim. To study the anti-inflammatory and liver regenerative effects of adipose-derived mesenchymal stem cells (ADSCs) on a porcine model of ischemia-reperfusion (IR) and hemihepatectomy. Methods. Eighteen healthy Bama miniature pigs were randomly divided into the sham-operated (sham), untreated IR injury (IRI), and ADSC-transplanted (ADSC) groups. Hepatic IR was established by laparoscopic hemihepatectomy. ADSCs were transplanted directly into the liver parenchyma after the surgery. Hepatic inflammation and liver regeneration were evaluated by histopathological examination and assessment of relevant cytokines and other factors. Results. ADSC transplantation successfully ameliorated the IRI-induced histopathological damage and the high levels of pro-inflammatory cytokines like IL-1β, IL-6, and TNF-α. In addition, the ADSCs enhanced the expression of the anti-inflammatory IL-10, regenerative factors including HGF, Cyclin D1, and proliferating cell nuclear antigen (PCNA), and angiogenic factors like VEGF, ANG-1, and ANG-2. Conclusions. ADSCs attenuated the hepatic IRI-induced inflammatory response and promoted liver regeneration. |
| format | Article |
| id | doaj-art-53747adf228e4276bd1f7f133b436a8d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-53747adf228e4276bd1f7f133b436a8d2025-02-03T06:44:21ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/24895842489584Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in SwineZhihui Jiao0Yajun Ma1Xiaoning Liu2Yansong Ge3Qianzhen Zhang4Boyang Liu5Hongbin Wang6Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaAim. To study the anti-inflammatory and liver regenerative effects of adipose-derived mesenchymal stem cells (ADSCs) on a porcine model of ischemia-reperfusion (IR) and hemihepatectomy. Methods. Eighteen healthy Bama miniature pigs were randomly divided into the sham-operated (sham), untreated IR injury (IRI), and ADSC-transplanted (ADSC) groups. Hepatic IR was established by laparoscopic hemihepatectomy. ADSCs were transplanted directly into the liver parenchyma after the surgery. Hepatic inflammation and liver regeneration were evaluated by histopathological examination and assessment of relevant cytokines and other factors. Results. ADSC transplantation successfully ameliorated the IRI-induced histopathological damage and the high levels of pro-inflammatory cytokines like IL-1β, IL-6, and TNF-α. In addition, the ADSCs enhanced the expression of the anti-inflammatory IL-10, regenerative factors including HGF, Cyclin D1, and proliferating cell nuclear antigen (PCNA), and angiogenic factors like VEGF, ANG-1, and ANG-2. Conclusions. ADSCs attenuated the hepatic IRI-induced inflammatory response and promoted liver regeneration.http://dx.doi.org/10.1155/2019/2489584 |
| spellingShingle | Zhihui Jiao Yajun Ma Xiaoning Liu Yansong Ge Qianzhen Zhang Boyang Liu Hongbin Wang Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine Stem Cells International |
| title | Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine |
| title_full | Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine |
| title_fullStr | Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine |
| title_full_unstemmed | Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine |
| title_short | Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine |
| title_sort | adipose derived stem cell transplantation attenuates inflammation and promotes liver regeneration after ischemia reperfusion and hemihepatectomy in swine |
| url | http://dx.doi.org/10.1155/2019/2489584 |
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