Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.)
The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.). Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monoox...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/421578 |
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| author | Jonggeun Kim Bosung Choi Young-Hwan Park Byoung-Kwan Cho Hyoun-Sub Lim Savithiry Natarajan Sang-Un Park Hanhong Bae |
| author_facet | Jonggeun Kim Bosung Choi Young-Hwan Park Byoung-Kwan Cho Hyoun-Sub Lim Savithiry Natarajan Sang-Un Park Hanhong Bae |
| author_sort | Jonggeun Kim |
| collection | DOAJ |
| description | The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.). Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF) encoding 518 amino acids (GenBank Accession number JX524278). The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78%) with that of Populus trichocarpa. Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old) of stem tissues, with a certain level of expression in all tissues tested. The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h), wounding (24 h), cold (24 h), abscisic acid (24 h), and methyl jasmonate (24 h). |
| format | Article |
| id | doaj-art-53532b3f316e458391a2095ca6c94ea4 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-53532b3f316e458391a2095ca6c94ea42025-02-03T05:44:43ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/421578421578Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.)Jonggeun Kim0Bosung Choi1Young-Hwan Park2Byoung-Kwan Cho3Hyoun-Sub Lim4Savithiry Natarajan5Sang-Un Park6Hanhong Bae7School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaDepartment of Biosystems and Machinery Engineering, Chungnam National University, Daejeon 305-764, Republic of KoreaDepartment of Applied Biology, Chungnam National University, Daejeon 305-764, Republic of KoreaSoybean Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USADepartment of Crop Science, Chungnam National University, Daejeon 305-754, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaThe purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.). Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF) encoding 518 amino acids (GenBank Accession number JX524278). The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78%) with that of Populus trichocarpa. Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old) of stem tissues, with a certain level of expression in all tissues tested. The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h), wounding (24 h), cold (24 h), abscisic acid (24 h), and methyl jasmonate (24 h).http://dx.doi.org/10.1155/2013/421578 |
| spellingShingle | Jonggeun Kim Bosung Choi Young-Hwan Park Byoung-Kwan Cho Hyoun-Sub Lim Savithiry Natarajan Sang-Un Park Hanhong Bae Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) The Scientific World Journal |
| title | Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) |
| title_full | Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) |
| title_fullStr | Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) |
| title_full_unstemmed | Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) |
| title_short | Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) |
| title_sort | molecular characterization of ferulate 5 hydroxylase gene from kenaf hibiscus cannabinus l |
| url | http://dx.doi.org/10.1155/2013/421578 |
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