Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification
Cibacron Blue F3GA immobilized as ligand onto epoxide chitosan/alginate composite was prepared and evaluated for the purification of human immunoglobulin G (IgG) isolated from human serum by dye-ligand affinity chromatography. The effect of buffer pH on IgG adsorption, kinetic and isothern adsorptio...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-09-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263-6174.30.8-9.701 |
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| author | Diego R. Gondim Luana P. Lima Maria C. M. de Souza Igor T. L. Bresolin Wellington S. Adriano Diana C. S. Azevedo Ivanildo J. Silva |
| author_facet | Diego R. Gondim Luana P. Lima Maria C. M. de Souza Igor T. L. Bresolin Wellington S. Adriano Diana C. S. Azevedo Ivanildo J. Silva |
| author_sort | Diego R. Gondim |
| collection | DOAJ |
| description | Cibacron Blue F3GA immobilized as ligand onto epoxide chitosan/alginate composite was prepared and evaluated for the purification of human immunoglobulin G (IgG) isolated from human serum by dye-ligand affinity chromatography. The effect of buffer pH on IgG adsorption, kinetic and isothern adsorption of high-purity IgG were studied by batch adsorption. The adsorption isotherm data were well fitted (using 25 mmol/L sodium phosphate buffer at pH 6.0) by the Langmuir–Freundlich model with a maximum adsorption capacity of 99 mg/g, apparent dissociation constant of 1.2 × 10 −6 mol/L and n equal to 4.86. Fixed-bed experiments were performed using high-purity IgG, achieving a recovery rate of 53%. Human serum diluted ten times was also injected into the column. An enrichment of IgG in the elution fraction with traces of human serum albumin was observed. According to the study results, this material can be applied in the purification of human IgG under optimized conditions. |
| format | Article |
| id | doaj-art-534435390e064e4ab50e98422ff4b203 |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-534435390e064e4ab50e98422ff4b2032025-02-03T10:08:09ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382012-09-013010.1260/0263-6174.30.8-9.701Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG PurificationDiego R. Gondim0Luana P. Lima1Maria C. M. de Souza2Igor T. L. Bresolin3Wellington S. Adriano4Diana C. S. Azevedo5Ivanildo J. Silva6 Grupo de Pesquisa em Separações por Adsorção (GPSA), Chemical Engineering Department, Federal University of Ceará, Campus Universitário do Pici, 60455-760 Fortaleza, CE, Brazil Grupo de Pesquisa em Separações por Adsorção (GPSA), Chemical Engineering Department, Federal University of Ceará, Campus Universitário do Pici, 60455-760 Fortaleza, CE, Brazil Grupo de Pesquisa em Separações por Adsorção (GPSA), Chemical Engineering Department, Federal University of Ceará, Campus Universitário do Pici, 60455-760 Fortaleza, CE, Brazil Department of Exact and Earth Sciences, Federal University of São Paulo (UNIFESP), Campus Diadema, 09913-030 Diadema, SP, Brazil Laboratory of Pharmaceutical Science, CES/UAS, Campina Grande Federal University, 58175-000 Cuite, PB, Brazil Grupo de Pesquisa em Separações por Adsorção (GPSA), Chemical Engineering Department, Federal University of Ceará, Campus Universitário do Pici, 60455-760 Fortaleza, CE, Brazil Grupo de Pesquisa em Separações por Adsorção (GPSA), Chemical Engineering Department, Federal University of Ceará, Campus Universitário do Pici, 60455-760 Fortaleza, CE, BrazilCibacron Blue F3GA immobilized as ligand onto epoxide chitosan/alginate composite was prepared and evaluated for the purification of human immunoglobulin G (IgG) isolated from human serum by dye-ligand affinity chromatography. The effect of buffer pH on IgG adsorption, kinetic and isothern adsorption of high-purity IgG were studied by batch adsorption. The adsorption isotherm data were well fitted (using 25 mmol/L sodium phosphate buffer at pH 6.0) by the Langmuir–Freundlich model with a maximum adsorption capacity of 99 mg/g, apparent dissociation constant of 1.2 × 10 −6 mol/L and n equal to 4.86. Fixed-bed experiments were performed using high-purity IgG, achieving a recovery rate of 53%. Human serum diluted ten times was also injected into the column. An enrichment of IgG in the elution fraction with traces of human serum albumin was observed. According to the study results, this material can be applied in the purification of human IgG under optimized conditions.https://doi.org/10.1260/0263-6174.30.8-9.701 |
| spellingShingle | Diego R. Gondim Luana P. Lima Maria C. M. de Souza Igor T. L. Bresolin Wellington S. Adriano Diana C. S. Azevedo Ivanildo J. Silva Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification Adsorption Science & Technology |
| title | Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification |
| title_full | Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification |
| title_fullStr | Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification |
| title_full_unstemmed | Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification |
| title_short | Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification |
| title_sort | dye ligand epoxide chitosan alginate a potential new stationary phase for human igg purification |
| url | https://doi.org/10.1260/0263-6174.30.8-9.701 |
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