Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer
Aim. Biochemical markers, including microRNAs (miRs), may facilitate the diagnosis and prognosis of breast cancer. This study was aimed at assessing serum miR-155 expression in patients with breast cancer and receptors. Methods. This case-control study was conducted on 36 patients with breast cancer...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2020/9514831 |
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| author | Fatemeh Hosseini Mojahed Amir Hossein Aalami Vahid Pouresmaeil Amir Amirabadi Mahdi Qasemi Rad Amirhossein Sahebkar |
| author_facet | Fatemeh Hosseini Mojahed Amir Hossein Aalami Vahid Pouresmaeil Amir Amirabadi Mahdi Qasemi Rad Amirhossein Sahebkar |
| author_sort | Fatemeh Hosseini Mojahed |
| collection | DOAJ |
| description | Aim. Biochemical markers, including microRNAs (miRs), may facilitate the diagnosis and prognosis of breast cancer. This study was aimed at assessing serum miR-155 expression in patients with breast cancer and receptors. Methods. This case-control study was conducted on 36 patients with breast cancer and 36 healthy individuals. After RNA extraction from the patient’s serum, cDNA was synthesized. The expression of miR-155 was measured using RT-qPCR. Demographic and histochemical data were extracted from patient documents. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software. Results. The mean age of subjects in breast cancer and control groups was 47.64±8.19 and 47.36±7.52 years, respectively. The serum miR-155 expression was higher in the cancer group (1.68±0.66) compared to the control group (p<0.0001). There was a significant relationship between serum miR-155 expression and the tumor grade (p<0.001), tumor stage (p<0.001), and tumor size (p<0.001) of the patients. However, no relationship between miR-155 expression and the presence of lymph node involvement (p=0.15), HER2 (p=0.79), Ki-67 (p=0.9), progesterone receptor (p=0.54), and estrogen receptors (p=0.84) was found. The ROC curve analysis showed that the AUC was 0.89 (77.78% sensitivity and 88.89% specificity), and the cutoff was 1.4 (Youden index: 0.6667) for detecting breast cancer. Conclusion. The findings of this study revealed that serum miR-155 may serve as a potential noninvasive molecular biomarker for breast cancer diagnosis and can help predict the grade of the disease. |
| format | Article |
| id | doaj-art-532c252e06d5451a8324f7fc0bdd79ca |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-532c252e06d5451a8324f7fc0bdd79ca2025-02-03T01:24:56ZengWileyInternational Journal of Genomics2314-436X2314-43782020-01-01202010.1155/2020/95148319514831Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast CancerFatemeh Hosseini Mojahed0Amir Hossein Aalami1Vahid Pouresmaeil2Amir Amirabadi3Mahdi Qasemi Rad4Amirhossein Sahebkar5Department of Medical Sciences, Mashhad Medical Sciences Branch, Islamic Azad University, Mashhad, IranDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, IranDepartment of Biochemistry, Mashhad Medical Sciences Branch, Islamic Azad University, Mashhad, IranSolid Tumors Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, IranHalal Research Center of IRI, FDA, Tehran, IranAim. Biochemical markers, including microRNAs (miRs), may facilitate the diagnosis and prognosis of breast cancer. This study was aimed at assessing serum miR-155 expression in patients with breast cancer and receptors. Methods. This case-control study was conducted on 36 patients with breast cancer and 36 healthy individuals. After RNA extraction from the patient’s serum, cDNA was synthesized. The expression of miR-155 was measured using RT-qPCR. Demographic and histochemical data were extracted from patient documents. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software. Results. The mean age of subjects in breast cancer and control groups was 47.64±8.19 and 47.36±7.52 years, respectively. The serum miR-155 expression was higher in the cancer group (1.68±0.66) compared to the control group (p<0.0001). There was a significant relationship between serum miR-155 expression and the tumor grade (p<0.001), tumor stage (p<0.001), and tumor size (p<0.001) of the patients. However, no relationship between miR-155 expression and the presence of lymph node involvement (p=0.15), HER2 (p=0.79), Ki-67 (p=0.9), progesterone receptor (p=0.54), and estrogen receptors (p=0.84) was found. The ROC curve analysis showed that the AUC was 0.89 (77.78% sensitivity and 88.89% specificity), and the cutoff was 1.4 (Youden index: 0.6667) for detecting breast cancer. Conclusion. The findings of this study revealed that serum miR-155 may serve as a potential noninvasive molecular biomarker for breast cancer diagnosis and can help predict the grade of the disease.http://dx.doi.org/10.1155/2020/9514831 |
| spellingShingle | Fatemeh Hosseini Mojahed Amir Hossein Aalami Vahid Pouresmaeil Amir Amirabadi Mahdi Qasemi Rad Amirhossein Sahebkar Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer International Journal of Genomics |
| title | Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer |
| title_full | Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer |
| title_fullStr | Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer |
| title_full_unstemmed | Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer |
| title_short | Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer |
| title_sort | clinical evaluation of the diagnostic role of microrna 155 in breast cancer |
| url | http://dx.doi.org/10.1155/2020/9514831 |
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