Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol
Banana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/5543104 |
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| author | Lesetja Moraba Legodi Daniel Coenrad LaGrange Elbert Lukas Jansen van Rensburg Ignatious Ncube |
| author_facet | Lesetja Moraba Legodi Daniel Coenrad LaGrange Elbert Lukas Jansen van Rensburg Ignatious Ncube |
| author_sort | Lesetja Moraba Legodi |
| collection | DOAJ |
| description | Banana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted in the availability of 52%, 48%, and 25% cellulose after treatment, respectively. Saccharification of the pretreated BPS with 10 FPU/g dry solids (29.3 mg protein/g d.s) crude enzyme from Trichoderma harzianum LMLBP07 13-5 at 50°C and a substrate loading of 10 to 15% released 3.8 to 21.8 g/L and from T. longibrachiatum LMLSAUL 14-1 released 5.4 to 43.5 g/L glucose to the biomass. Ethanol was produced through separate hydrolysis and fermentation (SHF) of alkaline pretreated BPS hydrolysate using Saccharomyces cerevisiae UL01 at 30°C and 100 rpm. Highest ethanol produced was 17.6 g/L. Banana pseudostem was shown as a potentially cheap substrate for bioethanol production. |
| format | Article |
| id | doaj-art-532b43ef43284f9098eaf8f5aca4469c |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-532b43ef43284f9098eaf8f5aca4469c2025-02-03T01:25:14ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/55431045543104Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce BioethanolLesetja Moraba Legodi0Daniel Coenrad LaGrange1Elbert Lukas Jansen van Rensburg2Ignatious Ncube3University of Limpopo, Department of Biochemistry Microbiology and Biotechnology, Private Bag X 1106, Sovenga 0727, South AfricaUniversity of Limpopo, Department of Biochemistry Microbiology and Biotechnology, Private Bag X 1106, Sovenga 0727, South AfricaUniversity of Limpopo, Department of Biochemistry Microbiology and Biotechnology, Private Bag X 1106, Sovenga 0727, South AfricaUniversity of Limpopo, Department of Biochemistry Microbiology and Biotechnology, Private Bag X 1106, Sovenga 0727, South AfricaBanana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted in the availability of 52%, 48%, and 25% cellulose after treatment, respectively. Saccharification of the pretreated BPS with 10 FPU/g dry solids (29.3 mg protein/g d.s) crude enzyme from Trichoderma harzianum LMLBP07 13-5 at 50°C and a substrate loading of 10 to 15% released 3.8 to 21.8 g/L and from T. longibrachiatum LMLSAUL 14-1 released 5.4 to 43.5 g/L glucose to the biomass. Ethanol was produced through separate hydrolysis and fermentation (SHF) of alkaline pretreated BPS hydrolysate using Saccharomyces cerevisiae UL01 at 30°C and 100 rpm. Highest ethanol produced was 17.6 g/L. Banana pseudostem was shown as a potentially cheap substrate for bioethanol production.http://dx.doi.org/10.1155/2021/5543104 |
| spellingShingle | Lesetja Moraba Legodi Daniel Coenrad LaGrange Elbert Lukas Jansen van Rensburg Ignatious Ncube Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol International Journal of Microbiology |
| title | Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol |
| title_full | Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol |
| title_fullStr | Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol |
| title_full_unstemmed | Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol |
| title_short | Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol |
| title_sort | enzymatic hydrolysis and fermentation of banana pseudostem hydrolysate to produce bioethanol |
| url | http://dx.doi.org/10.1155/2021/5543104 |
| work_keys_str_mv | AT lesetjamorabalegodi enzymatichydrolysisandfermentationofbananapseudostemhydrolysatetoproducebioethanol AT danielcoenradlagrange enzymatichydrolysisandfermentationofbananapseudostemhydrolysatetoproducebioethanol AT elbertlukasjansenvanrensburg enzymatichydrolysisandfermentationofbananapseudostemhydrolysatetoproducebioethanol AT ignatiousncube enzymatichydrolysisandfermentationofbananapseudostemhydrolysatetoproducebioethanol |