A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities
Recently, pharmaceutical and personal care products (PPCPs) have received considerable attention because of their increasing use. Analysis of PPCPs presents a significant analytical challenge, with high-performance liquid chromatography (HPLC) in reversed-phase mode, as the most widely used analytic...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2021-0014 |
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| author | Portillo-Castillo Omar J. Castro-Ríos Rocío Chávez-Montes Abelardo González-Horta Azucena Cavazos-Rocha Norma De Torres Noemí Waksman Garza-Tapia Marsela |
| author_facet | Portillo-Castillo Omar J. Castro-Ríos Rocío Chávez-Montes Abelardo González-Horta Azucena Cavazos-Rocha Norma De Torres Noemí Waksman Garza-Tapia Marsela |
| author_sort | Portillo-Castillo Omar J. |
| collection | DOAJ |
| description | Recently, pharmaceutical and personal care products (PPCPs) have received considerable attention because of their increasing use. Analysis of PPCPs presents a significant analytical challenge, with high-performance liquid chromatography (HPLC) in reversed-phase mode, as the most widely used analytical technique. To facilitate the optimization of the procedures that are applied in the early stages of sample preparation, a simple and fast HPLC method is proposed in this work for the separation of some PPCPs with a wide range of hydrophilicity. Two columns were evaluated (Atlantis dC18 and Discovery HS F5); as for mobile phases: a formate buffer (40 mmol L−1, pH 4) and methanol were tested in a gradient mode. The fluorinated column allowed better separation in a shorter time and better resolution for all analytes (Rs > 1). The proposed method delivered good performance for the tracing of PPCPs and is a suitable alternative to traditional C18-based HPLC methods. |
| format | Article |
| id | doaj-art-530c677e56aa4622910b30d84d1b2c8c |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-530c677e56aa4622910b30d84d1b2c8c2025-02-02T15:22:05ZengSciendoActa Pharmaceutica1846-95582021-06-0171230531510.2478/acph-2021-0014acph-2021-0014A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicitiesPortillo-Castillo Omar J.0Castro-Ríos Rocío1Chávez-Montes Abelardo2González-Horta Azucena3Cavazos-Rocha Norma4De Torres Noemí Waksman5Garza-Tapia Marsela6Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química, Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química, Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Química, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Ciencias Genómicas, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química, Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química, Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química, Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, MéxicoRecently, pharmaceutical and personal care products (PPCPs) have received considerable attention because of their increasing use. Analysis of PPCPs presents a significant analytical challenge, with high-performance liquid chromatography (HPLC) in reversed-phase mode, as the most widely used analytical technique. To facilitate the optimization of the procedures that are applied in the early stages of sample preparation, a simple and fast HPLC method is proposed in this work for the separation of some PPCPs with a wide range of hydrophilicity. Two columns were evaluated (Atlantis dC18 and Discovery HS F5); as for mobile phases: a formate buffer (40 mmol L−1, pH 4) and methanol were tested in a gradient mode. The fluorinated column allowed better separation in a shorter time and better resolution for all analytes (Rs > 1). The proposed method delivered good performance for the tracing of PPCPs and is a suitable alternative to traditional C18-based HPLC methods.https://doi.org/10.2478/acph-2021-0014pharmaceutical and personal care productsreversed-phase high-performance liquid chromatographyultraviolet detection |
| spellingShingle | Portillo-Castillo Omar J. Castro-Ríos Rocío Chávez-Montes Abelardo González-Horta Azucena Cavazos-Rocha Norma De Torres Noemí Waksman Garza-Tapia Marsela A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities Acta Pharmaceutica pharmaceutical and personal care products reversed-phase high-performance liquid chromatography ultraviolet detection |
| title | A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities |
| title_full | A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities |
| title_fullStr | A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities |
| title_full_unstemmed | A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities |
| title_short | A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities |
| title_sort | new rp hplc method as an auxiliary tool for optimization of sample preparation procedures for tracing of ppcps of different hydrophilicities |
| topic | pharmaceutical and personal care products reversed-phase high-performance liquid chromatography ultraviolet detection |
| url | https://doi.org/10.2478/acph-2021-0014 |
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