Gut microbiota of largemouth bass in response to temperature stress
The intestinal microbiota plays a crucial for maintaining host health, nutrient absorption, immune regulation, and pathogen resistance. This study investigated the effects of temperature stress on the intestinal microbiota of largemouth bass, an aquaculture species highly sensitive to temperature fl...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002984 |
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| author | Dongdong Wei Zhongbao Guo Fan Chen Hongfei Su Yibing Wang Lingling Deng Jing Huang Lin Huang Fei Ling Gaoxue Wang You Li Chengfan Lu Qing Yu Pengfei Li |
| author_facet | Dongdong Wei Zhongbao Guo Fan Chen Hongfei Su Yibing Wang Lingling Deng Jing Huang Lin Huang Fei Ling Gaoxue Wang You Li Chengfan Lu Qing Yu Pengfei Li |
| author_sort | Dongdong Wei |
| collection | DOAJ |
| description | The intestinal microbiota plays a crucial for maintaining host health, nutrient absorption, immune regulation, and pathogen resistance. This study investigated the effects of temperature stress on the intestinal microbiota of largemouth bass, an aquaculture species highly sensitive to temperature fluctuations. Fish were acclimated to 20°C and 35°C for two weeks, and then divided into four groups that were subjected to stable or sudden temperature changes (T20–20°C, T20→35°C, T35→20°C, T35–35°C). After a 7-day exposure, intestinal microbiota was analyzed via 16S rRNA gene high-throughput sequencing. Results showed that temperature stress significantly altered microbiota composition and diversity. The relative abundances of Cetobacterium, Epulopiscium, Citrobacter, and Aeromonas were significantly increased in the cold stress (T35→20°C) group and heat stress (T20→35°C) group. In T35–35°C group, the relative abundance of the harmful bacterium Edwardsiella was significantly greater than that in the other groups (P < 0.05). Temperature stress significantly altered intestinal microbiota diversity in largemouth bass. Temperature shifted from 35°C to 20°C increased bacterial network complexity, indicating enhanced cooperation for environmental adaptation. Sustained exposure to 35℃ reduced key species connectivity in co-occurrence networks, destabilizing the microbial community structure compared to 20℃ controls. Positive bacterial interactions were more prevalent at low temperatures (T20–20°C). Temperature stress primarily influenced gut microbiota assembly through stochastic processes, reducing homogenous dispersal and increasing pathogenic bacteria sensitivity. These results demonstrate that temperature stress disrupts intestinal microbiota homeostasis, impairing host health and aquaculture management. The findings provide critical insights for optimizing thermal management in aquaculture systems. |
| format | Article |
| id | doaj-art-5293c6260e0b45d98b6749b6e3dc57d6 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-5293c6260e0b45d98b6749b6e3dc57d62025-08-20T03:45:12ZengElsevierAquaculture Reports2352-51342025-09-014310291210.1016/j.aqrep.2025.102912Gut microbiota of largemouth bass in response to temperature stressDongdong Wei0Zhongbao Guo1Fan Chen2Hongfei Su3Yibing Wang4Lingling Deng5Jing Huang6Lin Huang7Fei Ling8Gaoxue Wang9You Li10Chengfan Lu11Qing Yu12Pengfei Li13Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR ChinaGuangxi Academy of Fishery Science, Nanning, Guangxi, PR ChinaCollege of Marine Sciences, Guangxi University, Nanning, PR ChinaCollege of Marine Sciences, Guangxi University, Nanning, PR ChinaCollege of Oceanography and Biotechnology, Guangxi University for Nationalities, Nanning, PR ChinaCollege of Oceanography and Biotechnology, Guangxi University for Nationalities, Nanning, PR ChinaGuangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR ChinaGuangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, PR ChinaLaibin Inspection and Testing Center, Laibin, PR ChinaLaibin Inspection and Testing Center, Laibin, PR ChinaGuangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China; Corresponding author.Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China; College of Marine Sciences, Guangxi University, Nanning, PR China; College of Oceanography and Biotechnology, Guangxi University for Nationalities, Nanning, PR China; Corresponding author at: Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China.The intestinal microbiota plays a crucial for maintaining host health, nutrient absorption, immune regulation, and pathogen resistance. This study investigated the effects of temperature stress on the intestinal microbiota of largemouth bass, an aquaculture species highly sensitive to temperature fluctuations. Fish were acclimated to 20°C and 35°C for two weeks, and then divided into four groups that were subjected to stable or sudden temperature changes (T20–20°C, T20→35°C, T35→20°C, T35–35°C). After a 7-day exposure, intestinal microbiota was analyzed via 16S rRNA gene high-throughput sequencing. Results showed that temperature stress significantly altered microbiota composition and diversity. The relative abundances of Cetobacterium, Epulopiscium, Citrobacter, and Aeromonas were significantly increased in the cold stress (T35→20°C) group and heat stress (T20→35°C) group. In T35–35°C group, the relative abundance of the harmful bacterium Edwardsiella was significantly greater than that in the other groups (P < 0.05). Temperature stress significantly altered intestinal microbiota diversity in largemouth bass. Temperature shifted from 35°C to 20°C increased bacterial network complexity, indicating enhanced cooperation for environmental adaptation. Sustained exposure to 35℃ reduced key species connectivity in co-occurrence networks, destabilizing the microbial community structure compared to 20℃ controls. Positive bacterial interactions were more prevalent at low temperatures (T20–20°C). Temperature stress primarily influenced gut microbiota assembly through stochastic processes, reducing homogenous dispersal and increasing pathogenic bacteria sensitivity. These results demonstrate that temperature stress disrupts intestinal microbiota homeostasis, impairing host health and aquaculture management. The findings provide critical insights for optimizing thermal management in aquaculture systems.http://www.sciencedirect.com/science/article/pii/S2352513425002984Intestinal microbiotaLargemouth bassTemperatureTemperature stress |
| spellingShingle | Dongdong Wei Zhongbao Guo Fan Chen Hongfei Su Yibing Wang Lingling Deng Jing Huang Lin Huang Fei Ling Gaoxue Wang You Li Chengfan Lu Qing Yu Pengfei Li Gut microbiota of largemouth bass in response to temperature stress Aquaculture Reports Intestinal microbiota Largemouth bass Temperature Temperature stress |
| title | Gut microbiota of largemouth bass in response to temperature stress |
| title_full | Gut microbiota of largemouth bass in response to temperature stress |
| title_fullStr | Gut microbiota of largemouth bass in response to temperature stress |
| title_full_unstemmed | Gut microbiota of largemouth bass in response to temperature stress |
| title_short | Gut microbiota of largemouth bass in response to temperature stress |
| title_sort | gut microbiota of largemouth bass in response to temperature stress |
| topic | Intestinal microbiota Largemouth bass Temperature Temperature stress |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002984 |
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