Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice
Fasting has become increasingly popular for treatment and prevention of obesity. Sex differences in the mechanisms of adaptation to fasting may contribute to choosing a therapeutic strategy for correction of metabolic disorders. Hepatokine fibroblast growth factor 21 (FGF21) is involved in the adapt...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-02-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1869 |
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| author | N. A. Feofanova T. V. Yakovleva E. N. Makarova N. M. Bazhan |
| author_facet | N. A. Feofanova T. V. Yakovleva E. N. Makarova N. M. Bazhan |
| author_sort | N. A. Feofanova |
| collection | DOAJ |
| description | Fasting has become increasingly popular for treatment and prevention of obesity. Sex differences in the mechanisms of adaptation to fasting may contribute to choosing a therapeutic strategy for correction of metabolic disorders. Hepatokine fibroblast growth factor 21 (FGF21) is involved in the adaptation to fasting. Muscles are assumed to be the main energy-consuming tissue in the body, as muscle metabolism plays an important role in the adaptation to nutritional deficit. However, there is still little information on sex differences in muscle and FGF21 physiological response to fasting. Our aim was to find out whether there were sex differences in hormonal regulation and the expression of genes controlling glucose and lipid metabolism in skeletal muscles in response to fasting. We estimated the effect of 24-hour fasting on the expression of genes involved in lipid (Ucp3, Cpt1) and carbohydrate (Slc2a4) metabolism in muscles and evaluated changes in body weight and blood plasma levels of glucose, insulin, free fatty acids (FFA), adiponectin, and FGF21 in male and female C57BL/6J mice. None of the genes studied (Ucp3, Cpt1 and Slc2a4) showed sex-related changes at mRNA levels in control groups, but females exposed to fasting demonstrated a significant increase in the expression of all genes as compared to control. Fasting significantly decreased body weight and glucose blood plasma levels in animals of both sexes but exerted no effect on the levels of insulin or FFA. The adiponectin and FGF21 levels were increased in response to fasting, the increase in females being significant. We were first to show sex dimorphism in muscle gene expression and FGF21 blood level in response to fasting. In females, the greater increase in FGF21 and adiponectin blood levels was positively associated with the greater upregulation of lipid oxidation and glucose uptake gene expression. |
| format | Article |
| id | doaj-art-527b9f46939c43efa416112655f9ab6b |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-02-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-527b9f46939c43efa416112655f9ab6b2025-02-01T09:58:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-02-01231626610.18699/VJ19.462872Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted miceN. A. Feofanova0T. V. Yakovleva1E. N. Makarova2N. M. Bazhan3Research Institute of Fundamental and Clinical Immunology; Institute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASFasting has become increasingly popular for treatment and prevention of obesity. Sex differences in the mechanisms of adaptation to fasting may contribute to choosing a therapeutic strategy for correction of metabolic disorders. Hepatokine fibroblast growth factor 21 (FGF21) is involved in the adaptation to fasting. Muscles are assumed to be the main energy-consuming tissue in the body, as muscle metabolism plays an important role in the adaptation to nutritional deficit. However, there is still little information on sex differences in muscle and FGF21 physiological response to fasting. Our aim was to find out whether there were sex differences in hormonal regulation and the expression of genes controlling glucose and lipid metabolism in skeletal muscles in response to fasting. We estimated the effect of 24-hour fasting on the expression of genes involved in lipid (Ucp3, Cpt1) and carbohydrate (Slc2a4) metabolism in muscles and evaluated changes in body weight and blood plasma levels of glucose, insulin, free fatty acids (FFA), adiponectin, and FGF21 in male and female C57BL/6J mice. None of the genes studied (Ucp3, Cpt1 and Slc2a4) showed sex-related changes at mRNA levels in control groups, but females exposed to fasting demonstrated a significant increase in the expression of all genes as compared to control. Fasting significantly decreased body weight and glucose blood plasma levels in animals of both sexes but exerted no effect on the levels of insulin or FFA. The adiponectin and FGF21 levels were increased in response to fasting, the increase in females being significant. We were first to show sex dimorphism in muscle gene expression and FGF21 blood level in response to fasting. In females, the greater increase in FGF21 and adiponectin blood levels was positively associated with the greater upregulation of lipid oxidation and glucose uptake gene expression.https://vavilov.elpub.ru/jour/article/view/1869musclegene expressionfastingmetabolismsex differencemicec57bl/6j strain |
| spellingShingle | N. A. Feofanova T. V. Yakovleva E. N. Makarova N. M. Bazhan Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice Вавиловский журнал генетики и селекции muscle gene expression fasting metabolism sex difference mice c57bl/6j strain |
| title | Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| title_full | Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| title_fullStr | Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| title_full_unstemmed | Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| title_short | Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| title_sort | sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice |
| topic | muscle gene expression fasting metabolism sex difference mice c57bl/6j strain |
| url | https://vavilov.elpub.ru/jour/article/view/1869 |
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