Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes

Intrauterine growth restriction (IUGR) is an important perinatal syndrome that poses several serious short- and long-term effects. We studied cytokine production by maternal peripheral blood lymphocytes stimulated by trophoblast antigens. 36 women with a diagnosis of IUGR and 22 healthy women with n...

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Main Authors: Raj Raghupathy, Majedah Al-Azemi, Fawaz Azizieh
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Clinical and Developmental Immunology
Online Access:http://dx.doi.org/10.1155/2012/734865
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author Raj Raghupathy
Majedah Al-Azemi
Fawaz Azizieh
author_facet Raj Raghupathy
Majedah Al-Azemi
Fawaz Azizieh
author_sort Raj Raghupathy
collection DOAJ
description Intrauterine growth restriction (IUGR) is an important perinatal syndrome that poses several serious short- and long-term effects. We studied cytokine production by maternal peripheral blood lymphocytes stimulated by trophoblast antigens. 36 women with a diagnosis of IUGR and 22 healthy women with normal fetal growth were inducted. Peripheral blood mononuclear cells were stimulated with trophoblast antigens and levels of the proinflammatory cytokines IL-6, IL-8, IL-12, IL-23, IFNγ, and TNFα and the anti-inflammatory cytokines IL-4, IL-10, and IL-13 were measured in culture supernatants by ELISA. IL-8 was produced at higher levels by blood cells of the IUGR group than normal pregnant women, while IL-13 was produced at lower levels. IL-8, IFNγ, and TNFα were higher in IUGR with placental insufficiency than in normal pregnancy. IL-12 levels were higher and IL-10 levels were lower in IUGR with placental insufficiency than in IUGR without placental insufficiency. We suggest that a stronger pro-inflammatory bias exists in IUGR as compared to normal pregnancy and in IUGR with placental insufficiency when compared to IUGR without placental insufficiency. Several ratios of proinflammatory to anti-inflammatory cytokines also support the existence of an inflammatory bias in IUGR.
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spelling doaj-art-526f2b9df9f045cfb11ccb7d46e861a12025-02-03T01:01:58ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/734865734865Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal LymphocytesRaj Raghupathy0Majedah Al-Azemi1Fawaz Azizieh2Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Kuwait 13110, KuwaitDepartment of Obstetrics & Gynecology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Kuwait 13110, KuwaitDepartment of Mathematics & Biology, Gulf University for Science and Technology, Mubarak Al-Abdullah Area, West Mishref, Hawalli 32093, KuwaitIntrauterine growth restriction (IUGR) is an important perinatal syndrome that poses several serious short- and long-term effects. We studied cytokine production by maternal peripheral blood lymphocytes stimulated by trophoblast antigens. 36 women with a diagnosis of IUGR and 22 healthy women with normal fetal growth were inducted. Peripheral blood mononuclear cells were stimulated with trophoblast antigens and levels of the proinflammatory cytokines IL-6, IL-8, IL-12, IL-23, IFNγ, and TNFα and the anti-inflammatory cytokines IL-4, IL-10, and IL-13 were measured in culture supernatants by ELISA. IL-8 was produced at higher levels by blood cells of the IUGR group than normal pregnant women, while IL-13 was produced at lower levels. IL-8, IFNγ, and TNFα were higher in IUGR with placental insufficiency than in normal pregnancy. IL-12 levels were higher and IL-10 levels were lower in IUGR with placental insufficiency than in IUGR without placental insufficiency. We suggest that a stronger pro-inflammatory bias exists in IUGR as compared to normal pregnancy and in IUGR with placental insufficiency when compared to IUGR without placental insufficiency. Several ratios of proinflammatory to anti-inflammatory cytokines also support the existence of an inflammatory bias in IUGR.http://dx.doi.org/10.1155/2012/734865
spellingShingle Raj Raghupathy
Majedah Al-Azemi
Fawaz Azizieh
Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
Clinical and Developmental Immunology
title Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
title_full Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
title_fullStr Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
title_full_unstemmed Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
title_short Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes
title_sort intrauterine growth restriction cytokine profiles of trophoblast antigen stimulated maternal lymphocytes
url http://dx.doi.org/10.1155/2012/734865
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AT majedahalazemi intrauterinegrowthrestrictioncytokineprofilesoftrophoblastantigenstimulatedmaternallymphocytes
AT fawazazizieh intrauterinegrowthrestrictioncytokineprofilesoftrophoblastantigenstimulatedmaternallymphocytes