Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae.
Streptococcus pneumoniae (SPN) is a significant pathogen causing pneumonia and meningitis, particularly in vulnerable populations like children and the elderly. Available pneumonia vaccines have limitations since they only cover particular serotypes and have high production costs. The emergence of a...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0317216 |
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| author | Md Nahian Muhammad Shahab Md Rasel Khan Shopnil Akash Tanjina Akhtar Banu Murshed Hasan Sarkar Barna Goswami Sanjana Fatema Chowdhury Mohammad Ariful Islam Ahmed Abu Rus'd Shamima Begum Ahashan Habib Aftab Ali Shaikh Jonas Ivan Nobre Oliveira Shahina Akter |
| author_facet | Md Nahian Muhammad Shahab Md Rasel Khan Shopnil Akash Tanjina Akhtar Banu Murshed Hasan Sarkar Barna Goswami Sanjana Fatema Chowdhury Mohammad Ariful Islam Ahmed Abu Rus'd Shamima Begum Ahashan Habib Aftab Ali Shaikh Jonas Ivan Nobre Oliveira Shahina Akter |
| author_sort | Md Nahian |
| collection | DOAJ |
| description | Streptococcus pneumoniae (SPN) is a significant pathogen causing pneumonia and meningitis, particularly in vulnerable populations like children and the elderly. Available pneumonia vaccines have limitations since they only cover particular serotypes and have high production costs. The emergence of antibiotic-resistant SPN strains further underscores the need for a new, cost-effective, broad-spectrum vaccine. Two potential vaccine candidates, CbpA and PspA, were identified, and their B-cell, CTL, and HTL epitopes were predicted and connected with suitable linkers, adjivant and PADRE sequence. The vaccine construct was found to be antigenic, non-toxic, non-allergenic, and soluble. The three-dimensional structure of the vaccine candidate was built and validated. Docking analysis of the vaccine candidate by ClusPro demonstrated robust and stable binding interactions between the MEV and toll-like receptor 4 in both humans and animals. The iMOD server and Amber v.22 tool has verified the stability of the docking complexes. GenScript server confirmed the high efficiency of cloning for the construct and in-silico cloning into the pET28a (+) vector using SnapGene, demonstrating successful translation of the epitope region. Immunological responses were shown to be enhanced by the C-IMMSIM server. This study introduced a strong peptide vaccine candidate that has the potential to contribute to the development of a rapid and cost-effective solution for combating SPN. However, experimental verification is necessary to evaluate the vaccine's effectiveness. |
| format | Article |
| id | doaj-art-52544bb6ff954a16a395e0bc70906bff |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-52544bb6ff954a16a395e0bc70906bff2025-02-05T05:31:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031721610.1371/journal.pone.0317216Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae.Md NahianMuhammad ShahabMd Rasel KhanShopnil AkashTanjina Akhtar BanuMurshed Hasan SarkarBarna GoswamiSanjana Fatema ChowdhuryMohammad Ariful IslamAhmed Abu Rus'dShamima BegumAhashan HabibAftab Ali ShaikhJonas Ivan Nobre OliveiraShahina AkterStreptococcus pneumoniae (SPN) is a significant pathogen causing pneumonia and meningitis, particularly in vulnerable populations like children and the elderly. Available pneumonia vaccines have limitations since they only cover particular serotypes and have high production costs. The emergence of antibiotic-resistant SPN strains further underscores the need for a new, cost-effective, broad-spectrum vaccine. Two potential vaccine candidates, CbpA and PspA, were identified, and their B-cell, CTL, and HTL epitopes were predicted and connected with suitable linkers, adjivant and PADRE sequence. The vaccine construct was found to be antigenic, non-toxic, non-allergenic, and soluble. The three-dimensional structure of the vaccine candidate was built and validated. Docking analysis of the vaccine candidate by ClusPro demonstrated robust and stable binding interactions between the MEV and toll-like receptor 4 in both humans and animals. The iMOD server and Amber v.22 tool has verified the stability of the docking complexes. GenScript server confirmed the high efficiency of cloning for the construct and in-silico cloning into the pET28a (+) vector using SnapGene, demonstrating successful translation of the epitope region. Immunological responses were shown to be enhanced by the C-IMMSIM server. This study introduced a strong peptide vaccine candidate that has the potential to contribute to the development of a rapid and cost-effective solution for combating SPN. However, experimental verification is necessary to evaluate the vaccine's effectiveness.https://doi.org/10.1371/journal.pone.0317216 |
| spellingShingle | Md Nahian Muhammad Shahab Md Rasel Khan Shopnil Akash Tanjina Akhtar Banu Murshed Hasan Sarkar Barna Goswami Sanjana Fatema Chowdhury Mohammad Ariful Islam Ahmed Abu Rus'd Shamima Begum Ahashan Habib Aftab Ali Shaikh Jonas Ivan Nobre Oliveira Shahina Akter Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. PLoS ONE |
| title | Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. |
| title_full | Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. |
| title_fullStr | Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. |
| title_full_unstemmed | Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. |
| title_short | Development of a broad-spectrum epitope-based vaccine against Streptococcus pneumoniae. |
| title_sort | development of a broad spectrum epitope based vaccine against streptococcus pneumoniae |
| url | https://doi.org/10.1371/journal.pone.0317216 |
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