Characterizing the hindgut microbiome in healthy and ketotic cows
Ketosis is a prevalent metabolic disease in the peripartum period of dairy cows, that causes considerable losses in milk production. In particular, the changes in the gut microbiome influence the occurrence of cow ketosis. In this study, the hindgut microbial communities of ketotic and healthy cows...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2393854 |
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| author | Zhihua Ju Rongling Li Wenhao Liu Qiang Jiang Jinpeng Wang Yaran Zhang Xiuge Wang Yao Xiao Chunhong Yang Xiaochao Wei Yaping Gao Jinming Huang |
| author_facet | Zhihua Ju Rongling Li Wenhao Liu Qiang Jiang Jinpeng Wang Yaran Zhang Xiuge Wang Yao Xiao Chunhong Yang Xiaochao Wei Yaping Gao Jinming Huang |
| author_sort | Zhihua Ju |
| collection | DOAJ |
| description | Ketosis is a prevalent metabolic disease in the peripartum period of dairy cows, that causes considerable losses in milk production. In particular, the changes in the gut microbiome influence the occurrence of cow ketosis. In this study, the hindgut microbial communities of ketotic and healthy cows were compared and analyzed using 16S rRNA sequencing. Community composition analysis reveal that the predominant phyl in both groups were Bacteroidetes and Firmicutes. At the genus level, Bifidobacterium were more abundant in healthy cows than in ketotic cows. Conversely, the ketotic group exhibited a greater abundance of Bacteroides and Treponema than the healthy group. Differential hindgut fecal bacterial taxa in healthy and ketotic cows were identified by linear discriminant analysis effect size, with increases in Treponema, Bacteroides, and Peptococcaceae and decreases in Christensenellales, Chloroflexi, and Paenibacillaceae in ketotic cows. A distance-based redundancy analysis was conducted to further explore the connection between the gut microbiome, blood β-hydroxybutyrate levels, and milk production traits. The results suggested that the hindgut microflora was significantly correlated with blood β-hydroxybutyrate concentration and somatic cell score. These results improve our understanding of rectal microbes, and may help prevent ketosis in cows. |
| format | Article |
| id | doaj-art-5242ecf473534e279915956a67bd4bba |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj-art-5242ecf473534e279915956a67bd4bba2025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.23938542393854Characterizing the hindgut microbiome in healthy and ketotic cowsZhihua Ju0Rongling Li1Wenhao Liu2Qiang Jiang3Jinpeng Wang4Yaran Zhang5Xiuge Wang6Yao Xiao7Chunhong Yang8Xiaochao Wei9Yaping Gao10Jinming Huang11Shandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesShandong Academy of Agricultural SciencesKetosis is a prevalent metabolic disease in the peripartum period of dairy cows, that causes considerable losses in milk production. In particular, the changes in the gut microbiome influence the occurrence of cow ketosis. In this study, the hindgut microbial communities of ketotic and healthy cows were compared and analyzed using 16S rRNA sequencing. Community composition analysis reveal that the predominant phyl in both groups were Bacteroidetes and Firmicutes. At the genus level, Bifidobacterium were more abundant in healthy cows than in ketotic cows. Conversely, the ketotic group exhibited a greater abundance of Bacteroides and Treponema than the healthy group. Differential hindgut fecal bacterial taxa in healthy and ketotic cows were identified by linear discriminant analysis effect size, with increases in Treponema, Bacteroides, and Peptococcaceae and decreases in Christensenellales, Chloroflexi, and Paenibacillaceae in ketotic cows. A distance-based redundancy analysis was conducted to further explore the connection between the gut microbiome, blood β-hydroxybutyrate levels, and milk production traits. The results suggested that the hindgut microflora was significantly correlated with blood β-hydroxybutyrate concentration and somatic cell score. These results improve our understanding of rectal microbes, and may help prevent ketosis in cows.http://dx.doi.org/10.1080/26895293.2024.2393854cow ketosisβ-hydroxybutyrate16s sequencinghindgut fecal microbiota |
| spellingShingle | Zhihua Ju Rongling Li Wenhao Liu Qiang Jiang Jinpeng Wang Yaran Zhang Xiuge Wang Yao Xiao Chunhong Yang Xiaochao Wei Yaping Gao Jinming Huang Characterizing the hindgut microbiome in healthy and ketotic cows All Life cow ketosis β-hydroxybutyrate 16s sequencing hindgut fecal microbiota |
| title | Characterizing the hindgut microbiome in healthy and ketotic cows |
| title_full | Characterizing the hindgut microbiome in healthy and ketotic cows |
| title_fullStr | Characterizing the hindgut microbiome in healthy and ketotic cows |
| title_full_unstemmed | Characterizing the hindgut microbiome in healthy and ketotic cows |
| title_short | Characterizing the hindgut microbiome in healthy and ketotic cows |
| title_sort | characterizing the hindgut microbiome in healthy and ketotic cows |
| topic | cow ketosis β-hydroxybutyrate 16s sequencing hindgut fecal microbiota |
| url | http://dx.doi.org/10.1080/26895293.2024.2393854 |
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