Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard
Today, the general public has become increasingly aware of salmonellosis problems. Organic acids are known by their antimicrobial potential and commonly used for improving the quality of poultry feed. In this context, the present work evaluated the inhibitory effect of four organic acids, namely, ac...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/7352593 |
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| author | Aicha El Baaboua Mohamed El Maadoudi Abdelhakim Bouyahya Omar Belmehdi Ayoub Kounnoun Rajae Zahli Jamal Abrini |
| author_facet | Aicha El Baaboua Mohamed El Maadoudi Abdelhakim Bouyahya Omar Belmehdi Ayoub Kounnoun Rajae Zahli Jamal Abrini |
| author_sort | Aicha El Baaboua |
| collection | DOAJ |
| description | Today, the general public has become increasingly aware of salmonellosis problems. Organic acids are known by their antimicrobial potential and commonly used for improving the quality of poultry feed. In this context, the present work evaluated the inhibitory effect of four organic acids, namely, acetic acid, citric acid, lactic acid, and tartaric acid, at different levels of contamination by Salmonella typhimurium. The neutralization of these organic acids in vitro and in the presence of one-day-old chick’s organs was also investigated during the search for Salmonella serovars in birds as described in the Moroccan standard “NM 08.0.550.” The effect of four organic acids on Salmonella typhimurium was tested in vitro and in the presence of chick’s organs at different concentrations set of strain and organic acids tested. The MIC results demonstrated that tartaric acid, citric acid, and acetic acid inhibited Salmonella typhimurium at concentrations of 0.312%, 0.625%, and 0.512% for the three levels of strain: 10, 100, and 103 CFU/ml, respectively, while lactic acid and depending on the amount of the strain introduced acts differently: 0.078% for 10 CFU/ml and 0.156% for 100 and 103 CFU/ml. The concentration of 0.04M of Na2HPO4 solution has proved, in vitro, in caecums and organs of chicks (in presence of organic acids) that strain introduced, even at low concentrations, can be recovered. The use of additives has beneficial effects in Salmonella control program. However, the present results recommend the amendment of Salmonella research standard, taking into account the probable presence of organic acids in digestive content of one-day-old chicks. |
| format | Article |
| id | doaj-art-51faeaf77b744287be2d14a7db995ed2 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-51faeaf77b744287be2d14a7db995ed22025-02-03T01:25:42ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/73525937352593Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search StandardAicha El Baaboua0Mohamed El Maadoudi1Abdelhakim Bouyahya2Omar Belmehdi3Ayoub Kounnoun4Rajae Zahli5Jamal Abrini6Biology and Health Laboratory, Biotechnology and Applied Microbiology Team, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, MoroccoRegional Laboratory for Analysis and Research, National Office for Food Safety, Tangier, MoroccoLaboratory of Human Pathology Biology, Faculty of Sciences, and Genomic Center of Human Pathology, Mohammed V University, Rabat, MoroccoBiology and Health Laboratory, Biotechnology and Applied Microbiology Team, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, MoroccoRegional Laboratory for Analysis and Research, National Office for Food Safety, Tangier, MoroccoBiology and Health Laboratory, Biotechnology and Applied Microbiology Team, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, MoroccoBiology and Health Laboratory, Biotechnology and Applied Microbiology Team, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, MoroccoToday, the general public has become increasingly aware of salmonellosis problems. Organic acids are known by their antimicrobial potential and commonly used for improving the quality of poultry feed. In this context, the present work evaluated the inhibitory effect of four organic acids, namely, acetic acid, citric acid, lactic acid, and tartaric acid, at different levels of contamination by Salmonella typhimurium. The neutralization of these organic acids in vitro and in the presence of one-day-old chick’s organs was also investigated during the search for Salmonella serovars in birds as described in the Moroccan standard “NM 08.0.550.” The effect of four organic acids on Salmonella typhimurium was tested in vitro and in the presence of chick’s organs at different concentrations set of strain and organic acids tested. The MIC results demonstrated that tartaric acid, citric acid, and acetic acid inhibited Salmonella typhimurium at concentrations of 0.312%, 0.625%, and 0.512% for the three levels of strain: 10, 100, and 103 CFU/ml, respectively, while lactic acid and depending on the amount of the strain introduced acts differently: 0.078% for 10 CFU/ml and 0.156% for 100 and 103 CFU/ml. The concentration of 0.04M of Na2HPO4 solution has proved, in vitro, in caecums and organs of chicks (in presence of organic acids) that strain introduced, even at low concentrations, can be recovered. The use of additives has beneficial effects in Salmonella control program. However, the present results recommend the amendment of Salmonella research standard, taking into account the probable presence of organic acids in digestive content of one-day-old chicks.http://dx.doi.org/10.1155/2018/7352593 |
| spellingShingle | Aicha El Baaboua Mohamed El Maadoudi Abdelhakim Bouyahya Omar Belmehdi Ayoub Kounnoun Rajae Zahli Jamal Abrini Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard International Journal of Microbiology |
| title | Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard |
| title_full | Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard |
| title_fullStr | Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard |
| title_full_unstemmed | Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard |
| title_short | Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard |
| title_sort | evaluation of antimicrobial activity of four organic acids used in chicks feed to control salmonella typhimurium suggestion of amendment in the search standard |
| url | http://dx.doi.org/10.1155/2018/7352593 |
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