Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum)
Pomfret fish (Colossoma macropomum) is a highly valuable freshwater fishery commodity. Bacterial diseases, particularly those caused by Aeromonas hydrophila bacteria, are a common issue in these fish. Using immunostimulants to control bacterial diseases can enhance the function of the immune system....
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03027.pdf |
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| author | Ismarica Ismarica Helda Musfika Dara Dewi Cut |
| author_facet | Ismarica Ismarica Helda Musfika Dara Dewi Cut |
| author_sort | Ismarica Ismarica |
| collection | DOAJ |
| description | Pomfret fish (Colossoma macropomum) is a highly valuable freshwater fishery commodity. Bacterial diseases, particularly those caused by Aeromonas hydrophila bacteria, are a common issue in these fish. Using immunostimulants to control bacterial diseases can enhance the function of the immune system. Garlic is a natural ingredient that can serve as an effective immunostimulant. This study aims to assess the impact of adding garlic extract to feed on the survival and blood profile of Colossoma macropomum. The research employed an experimental method using a completely randomized design (CRD) with 6 treatments and 3 repetitions. The doses of garlic extract used were P1 (0 g/kg control +), P2 (0 g/kg control -), P3 (10 g/kg), P4 (15 g/kg), P5 (20 g/kg), and P6 (20 g/kg). A total of 180 pomfret fish were used in the study, and they were fed with additional garlic extract for 28 days. Subsequently, the fish were infected with Aeromonas hydrophila bacteria at a specific density. The results of the ANOVA test indicated that the provision of garlic extract had a significant effect on leukocytes (1,99±10388,73 x105 sel/mm3) and erythrocytes (10,10±0,25x106 sel/mm3 ) at H-15 (after bacterial infection) and leukocytes (1,43±9196,33x106 sel/mm3 )at H-28. Furthermore, the use of garlic extract in feed was shown to have a significant effect (P<0.05) on the survival of the fish, with the best survival rate at 83.33±5.77% in treatment P3 (10 g/kg). The study also identified the optimal dose of garlic extract for use as an immunostimulant, which was determined to be P3 (10g/kg). |
| format | Article |
| id | doaj-art-51eaaf028c8a48de8ebfd4dd6471c359 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-51eaaf028c8a48de8ebfd4dd6471c3592025-02-05T10:43:33ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011560302710.1051/bioconf/202515603027bioconf_icfaes24_03027Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum)Ismarica Ismarica0Helda Musfika1Dara Dewi Cut2Aquaculture Department, Faculty of Marine and Fisheries, Universitas Syiah KualaAquaculture Department, Faculty of Marine and Fisheries, Universitas Syiah KualaAquaculture Department, Faculty of Marine and Fisheries, Universitas Syiah KualaPomfret fish (Colossoma macropomum) is a highly valuable freshwater fishery commodity. Bacterial diseases, particularly those caused by Aeromonas hydrophila bacteria, are a common issue in these fish. Using immunostimulants to control bacterial diseases can enhance the function of the immune system. Garlic is a natural ingredient that can serve as an effective immunostimulant. This study aims to assess the impact of adding garlic extract to feed on the survival and blood profile of Colossoma macropomum. The research employed an experimental method using a completely randomized design (CRD) with 6 treatments and 3 repetitions. The doses of garlic extract used were P1 (0 g/kg control +), P2 (0 g/kg control -), P3 (10 g/kg), P4 (15 g/kg), P5 (20 g/kg), and P6 (20 g/kg). A total of 180 pomfret fish were used in the study, and they were fed with additional garlic extract for 28 days. Subsequently, the fish were infected with Aeromonas hydrophila bacteria at a specific density. The results of the ANOVA test indicated that the provision of garlic extract had a significant effect on leukocytes (1,99±10388,73 x105 sel/mm3) and erythrocytes (10,10±0,25x106 sel/mm3 ) at H-15 (after bacterial infection) and leukocytes (1,43±9196,33x106 sel/mm3 )at H-28. Furthermore, the use of garlic extract in feed was shown to have a significant effect (P<0.05) on the survival of the fish, with the best survival rate at 83.33±5.77% in treatment P3 (10 g/kg). The study also identified the optimal dose of garlic extract for use as an immunostimulant, which was determined to be P3 (10g/kg).https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03027.pdf |
| spellingShingle | Ismarica Ismarica Helda Musfika Dara Dewi Cut Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) BIO Web of Conferences |
| title | Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) |
| title_full | Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) |
| title_fullStr | Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) |
| title_full_unstemmed | Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) |
| title_short | Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum) |
| title_sort | addition of garlic allium sativum extract in fry as immunostimulant on survival and blood profile of pomfret fish colossoma macropomum |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03027.pdf |
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